Restriction map for
pF1KE3483
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BbeI BbrPI BcgI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoRI FseI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 5011 CGCG CG^CG
AflIII 27 ACATGT A^C[GA][CT]GT
Aor51HI 2768 AGCGCT AGC^GCT
AviII 3665 TGCGCA TGC^GCA
BanI 105 GGCACC G^G[CT][GA]CC
BbvCI 3686 CCTCAGC CCTCAGC(-5/-2)
BciVI 477 GGATAC GTATCC(6/5)
BlpI 264 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTTAGC GC^T[ACGT]AGC
BspLU11I 27 ACATGT A^CATGT
BsrDI 122 GCAATG GCAATG(2/0)
BstUI 5011 CGCG CG^CG
CelII 264 GCTTAGC GC^T[ACGT]AGC
DraIII 4372 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2768 AGCGCT AGC^GCT
FspI 3665 TGCGCA TGC^GCA
HpaI 1477 GTTAAC GTT^AAC
MvnI 5011 CGCG CG^CG
NaeI 3767 GCCGGC GCC^GGC
NgoMIV 3767 GCCGGC G^CCGGC
PaeR7I 1465 CTCGAG C^TCGAG
PpuMI 3509 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 4168 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 4904 CCCGGG CCC^GGG
SpeI 1654 ACTAGT A^CTAGT
StuI 3658 AGGCCT AGG^CCT
XbaI 3337 TCTAGA T^CTAGA
XhoI 1465 CTCGAG C^TCGAG
XmaCI 4904 CCCGGG C^CCGGG
XmaI 4904 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 4270 GACGCC G[GA]^CG[CT]C
4972 GACGCC
AhdI 3631 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3964 GACAGCTGGTC
AspEI 3631 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3964 GACAGCTGGTC
BclI 3476 TGATCA T^GATCA
5108 TGATCA
BglI 2439 GCCAGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2861 GCCTGGATGGC
BglII 4076 AGATCT A^GATCT
5016 AGATCT
BsaHI 4270 GACGCC G[GA]^CG[CT]C
4972 GACGCC
BsgI 3216 CTGCAC GTGCAG(16/14)
4807 CTGCAC
Bsp1407I 1287 TGTACA T^GTACA
2969 TGTACA
BsrBI 4890 CCGCTC CCGCTC(-3/-3)
4954 GAGCGG
BsrFI 668 GCCGGT [GA]^CCGG[CT]
3767 GCCGGC
BsrGI 1287 TGTACA T^GTACA
2969 TGTACA
BssSI 745 CACGAG CACGAG(-5/-1)
4862 CACGAG
BstEII 1114 GGTAACC G^GT[ACGT]ACC
2647 GGTAACC
BstPI 1114 GGTAACC G^GT[ACGT]ACC
2647 GGTAACC
BstXI 3743 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4599 CCAAGGCTTTGG
Cfr10I 668 GCCGGT [GA]^CCGG[CT]
3767 GCCGGC
DraI 61 TTTAAA TTT^AAA
2782 TTTAAA
Eam1105I 3631 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3964 GACAGCTGGTC
EcoNI 2983 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3650 CCTTGGAAAGG
EcoO65I 1114 GGTAACC G^GT[ACGT]ACC
2647 GGTAACC
EcoRV 2775 GATATC GAT^ATC
3565 GATATC
EcoT22I 1898 ATGCAT ATGCA^T
3065 ATGCAT
FbaI 3476 TGATCA T^GATCA
5108 TGATCA
HaeII 2768 AGCGCT [GA]GCGC^[CT]
2906 AGCGCC
HgaI 4270 GACGC GACGC(5/10)
4972 GACGC
Hin1I 4270 GACGCC G[GA]^CG[CT]C
4972 GACGCC
HincII 1477 GTTAAC GT[CT]^[GA]AC
2703 GTCAAC
HindII 1477 GTTAAC GT[CT]^[GA]AC
2703 GTCAAC
MaeII 1056 ACGT A^CGT
4373 ACGT
NdeI 1901 CATATG CA^TATG
4040 CATATG
NsiI 1898 ATGCAT ATGCA^T
3065 ATGCAT
PflMI 4371 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4689 CCATCTGGTGG
SexAI 39 ACCTGGT A^CC[AT]GGT
2698 ACCAGGT
SspBI 1287 TGTACA T^GTACA
2969 TGTACA
Van91I 4371 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4689 CCATCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BamHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII