Restriction map for
pF1KE3451
##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccI AclI AcsI AgeI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BlnI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1884 GACGTC GACGT^C
AfaI 546 GTAC GT^AC
AflII 22 CTTAAG C^TTAAG
AflIII 1538 ACGTGT A^C[GA][CT]GT
AhdI 661 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2791 GTGCAC G^TGCAC
Aor51HI 2863 AGCGCT AGC^GCT
ApaLI 2791 GTGCAC G^TGCAC
AspEI 661 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1998 GGCGCC GGCGC^C
BbsI 2826 GTCTTC GAAGAC(2/6)
BciVI 3164 GTATCC GTATCC(6/5)
BfaI 10 CTAG C^TAG
BfrI 22 CTTAAG C^TTAAG
BpuAI 2826 GTCTTC GAAGAC(2/6)
BsaAI 82 TACGTG [CT]AC^GT[GA]
BsaBI 1233 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1104 GAATGC GAATGC(1/-1)
DraIII 436 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 661 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2863 AGCGCT AGC^GCT
HincII 1213 GTCAAC GT[CT]^[GA]AC
HindII 1213 GTCAAC GT[CT]^[GA]AC
KasI 1998 GGCGCC G^GCGCC
KspI 3639 CCGCGG CCGC^GG
MaeI 10 CTAG C^TAG
MamI 1233 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1998 GGCGCC GG^CGCC
NotI 13 GCGGCCGC GC^GGCCGC
PshAI 1206 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 546 GTAC GT^AC
SacI 1651 GAGCTC GAGCT^C
SacII 3639 CCGCGG CCGC^GG
SfoI 1998 GGCGCC GGC^GCC
TfiI 3465 GATTC G^A[AT]TC
Tsp509I 1116 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 1229 TCCGGA T^CCGGA
2114 TCCGGA
BalI 3026 TGGCCA TGG^CCA
3110 TGGCCA
BbvCI 2599 CCTCAGC CCTCAGC(-5/-2)
1597 GCTGAGG
BclI 749 TGATCA T^GATCA
863 TGATCA
BglII 140 AGATCT A^GATCT
410 AGATCT
BsaI 2069 GAGACC GGTCTC(1/5)
3364 GAGACC
BseAI 1229 TCCGGA T^CCGGA
2114 TCCGGA
BsiEI 14 CGGCCG CG[GA][CT]^CG
3250 CGGCCG
BsmBI 1645 GAGACG CGTCTC(1/5)
1882 GAGACG
BspEI 1229 TCCGGA T^CCGGA
2114 TCCGGA
BssHII 3724 GCGCGC G^CGCGC
3909 GCGCGC
BssSI 960 CACGAG CACGAG(-5/-1)
2027 CACGAG
EagI 14 CGGCCG C^GGCCG
3250 CGGCCG
EclXI 14 CGGCCG C^GGCCG
3250 CGGCCG
Eco52I 14 CGGCCG C^GGCCG
3250 CGGCCG
EcoNI 2698 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3422 CCTTGCCCAGG
FbaI 749 TGATCA T^GATCA
863 TGATCA
MluNI 3026 TGGCCA TGG^CCA
3110 TGGCCA
MroI 1229 TCCGGA T^CCGGA
2114 TCCGGA
MscI 3026 TGGCCA TGG^CCA
3110 TGGCCA
MseI 23 TTAA T^TAA
305 TTAA
PflMI 435 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCAGTGCCTGG
SapI 1744 GAAGAGC GCTCTTC(1/4)
3432 GAAGAGC
SfiI 2457 GGCCTCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3315 GGCCTCAGGGGCC
SphI 1796 GCATGC GCATG^C
3488 GCATGC
StuI 917 AGGCCT AGG^CCT
1460 AGGCCT
Tru9I 23 TTAA T^TAA
305 TTAA
Van91I 435 CCACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1033 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI