Restriction map for
pF1KB5986
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspHI AspI AviII AvrII
BalI BbeI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII CfoI CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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Acc65I 1604 GGTACC G^GTACC
AciI 1539 CCGC CCGC(-3/-1)
AlwNI 1648 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1234 CCTGAGG CC^T[ACGT]AGG
AseI 1318 ATTAAT AT^TAAT
AsnI 1318 ATTAAT AT^TAAT
Asp718I 1604 GGTACC G^GTACC
AvaII 1564 GGACC G^G[AT]CC
BamHI 721 GGATCC G^GATCC
BanI 1604 GGTACC G^G[CT][GA]CC
BanII 687 GAGCCC G[GA]GC[CT]^C
BbrPI 1693 CACGTG CAC^GTG
BbvI 964 GCAGC GCAGC(8/12)
BciVI 1170 GTATCC GTATCC(6/5)
BsaAI 1693 CACGTG [CT]AC^GT[GA]
BsaJI 629 CCCGGG C^C[ACGT][ACGT]GG
BsmBI 407 GAGACG CGTCTC(1/5)
BspDI 1503 ATCGAT AT^CGAT
BsrDI 768 CATTGC GCAATG(2/0)
BsrFI 751 ACCGGC [GA]^CCGG[CT]
BstEII 1628 GGTCACC G^GT[ACGT]ACC
BstNI 215 CCAGG CC^[AT]GG
BstPI 1628 GGTCACC G^GT[ACGT]ACC
BstYI 721 GGATCC [GA]^GATC[CT]
Bsu36I 1234 CCTGAGG CC^T[ACGT]AGG
Cfr10I 751 ACCGGC [GA]^CCGG[CT]
Cfr13I 1564 GGACC G^G[ACGT]CC
ClaI 1503 ATCGAT AT^CGAT
EaeI 753 CGGCCA [CT]^GGCC[GA]
EarI 1405 GAAGAG CTCTTC(1/4)
Eco81I 1234 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1628 GGTCACC G^GT[ACGT]ACC
EcoRII 215 CCAGG ^CC[AT]GG
EcoT22I 590 ATGCAT ATGCA^T
Fnu4HI 964 GCAGC GC^[ACGT]GC
HgaI 1156 GACGC GACGC(5/10)
HincII 1032 GTTGAC GT[CT]^[GA]AC
HindII 1032 GTTGAC GT[CT]^[GA]AC
HindIII 1133 AAGCTT A^AGCTT
ItaI 964 GCAGC GC^[ACGT]GC
KpnI 1604 GGTACC GGTAC^C
Ksp632I 1405 GAAGAG CTCTTC(1/4)
MfeI 733 CAATTG C^AATTG
MflI 721 GGATCC [GA]^GATC[CT]
MunI 733 CAATTG C^AATTG
MvaI 215 CCAGG CC^[AT]GG
MwoI 1614 GCCAGTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 590 ATGCAT ATGCA^T
NspI 1610 ACATGC [GA]CATG^[CT]
PleI 527 GAGTC GAGTC(4/5)
PmaCI 1693 CACGTG CAC^GTG
PmlI 1693 CACGTG CAC^GTG
PshAI 1565 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1318 ATTAAT AT^TAAT
PvuII 1383 CAGCTG CAG^CTG
SapI 1405 GAAGAGC GCTCTTC(1/4)
Sau96I 1564 GGACC G^G[ACGT]CC
SfcI 200 CTACAG C^T[GA][CT]AG
SmaI 629 CCCGGG CCC^GGG
SmlI 1462 CTTGAG C^T[CT][GA]AG
StuI 673 AGGCCT AGG^CCT
TaqI 1504 TCGA T^CGA
TseI 964 GCAGC G^C[AT]GC
TthHB8I 1504 TCGA T^CGA
XbaI 651 TCTAGA T^CTAGA
XhoII 721 GGATCC [GA]^GATC[CT]
XmaCI 629 CCCGGG C^CCGGG
XmaI 629 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 712 GTAGAC GT^[AC][GT]AC
1194 GTCTAC
AlwI 722 GATCC GGATC(4/5)
721 GGATC
AvaI 629 CCCGGG C^[CT]CG[GA]G
1702 CTCGGG
BcnI 629 CCCGG CC^[GC]GG
630 CCGGG
BfaI 652 CTAG C^TAG
820 CTAG
BmyI 423 GTGCCC G[AGT]GC[ACT]^C
687 GAGCCC
BsmAI 407 GAGAC GTCTC(1/5)
700 GAGAC
BsoBI 629 CCCGGG C^[CT]CG[GA]G
1702 CTCGGG
Bsp1286I 423 GTGCCC G[AGT]GC[ACT]^C
687 GAGCCC
Cac8I 611 GCAAGC GC[ACGT]^[ACGT]GC
1385 GCTGGC
DpnII 722 GATC ^GATC
738 GATC
DraI 442 TTTAAA TTT^AAA
1222 TTTAAA
HaeIII 674 GGCC GG^CC
754 GGCC
HapII 630 CCGG C^CGG
752 CCGG
HpaII 630 CCGG C^CGG
752 CCGG
MaeI 652 CTAG C^TAG
820 CTAG
MboI 722 GATC ^GATC
738 GATC
MspA1I 1383 CAGCTG C[AC]G^C[GT]G
1539 CCGCTG
MspI 630 CCGG C^CGG
752 CCGG
NciI 629 CCCGG CC^[GC]GG
630 CCGGG
NdeII 722 GATC ^GATC
738 GATC
Sau3AI 722 GATC ^GATC
738 GATC
SfaNI 961 GATGC GCATC(5/9)
592 GCATC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BpmI BpuAI BsiYI BslI BsmFI
BsrI BssKI BstF5I DdeI FokI
HinfI HphI MaeII MaeIII MboII
MnlI MseI MslI NlaIII NlaIV
RsaI ScrFI TfiI Tru9I Tsp45I
Tsp509I TspRI