Restriction map for
pF1KE9610
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbsI BbvCI BcgI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 136 GTCTAC GT^[AC][GT]AC
AcyI 94 GGCGTC G[GA]^CG[CT]C
AspHI 664 GAGCAC G[AT]GC[AT]^C
AspI 259 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 667 CACGTG CAC^GTG
BciVI 606 GGATAC GTATCC(6/5)
BglI 544 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 174 AGATCT A^GATCT
BsaAI 667 CACGTG [CT]AC^GT[GA]
BsaHI 94 GGCGTC G[GA]^CG[CT]C
BsaWI 701 TCCGGT [AT]^CCGG[AT]
BsiHKAI 664 GAGCAC G[AT]GC[AT]^C
BsrBI 223 GAGCGG CCGCTC(-3/-3)
EcoT22I 380 ATGCAT ATGCA^T
Hin1I 94 GGCGTC G[GA]^CG[CT]C
KspI 62 CCGCGG CCGC^GG
NcoI 131 CCATGG C^CATGG
NsiI 380 ATGCAT ATGCA^T
NspI 311 GCATGC [GA]CATG^[CT]
PflFI 259 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
PpuMI 826 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 708 CAGCTG CAG^CTG
SacII 62 CCGCGG CCGC^GG
SphI 311 GCATGC GCATG^C
TaqI 98 TCGA T^CGA
TfiI 780 GAATC G^A[AT]TC
TspRI 363 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 259 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 98 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 14 AGCT AG^CT
709 AGCT
AlwI 637 GATCC GGATC(4/5)
3 GGATC
AlwNI 359 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
534 CAGCATCTG
ApaI 114 GGGCCC GGGCC^C
126 GGGCCC
AvaI 472 CCCGGG C^[CT]CG[GA]G
554 CCCGGG
BanII 114 GGGCCC G[GA]GC[CT]^C
126 GGGCCC
BsoBI 472 CCCGGG C^[CT]CG[GA]G
554 CCCGGG
BsrI 364 ACTGG ACTGG(1/-1)
693 CCAGT
BstYI 3 GGATCT [GA]^GATC[CT]
174 AGATCT
DsaI 62 CCGCGG C^C[GA][CT]GG
131 CCATGG
EcoT14I 131 CCATGG C^C[AT][AT]GG
545 CCAAGG
HaeII 183 GGCGCT [GA]GCGC^[CT]
273 GGCGCT
HgaI 86 GCGTC GACGC(5/10)
95 GCGTC
HphI 483 GGTGA GGTGA(8/7)
404 TCACC
MaeII 401 ACGT A^CGT
668 ACGT
MflI 3 GGATCT [GA]^GATC[CT]
174 AGATCT
PstI 457 CTGCAG CTGCA^G
595 CTGCAG
SfcI 457 CTGCAG C^T[GA][CT]AG
595 CTGCAG
SmaI 472 CCCGGG CCC^GGG
554 CCCGGG
StyI 131 CCATGG C^C[AT][AT]GG
545 CCAAGG
XhoII 3 GGATCT [GA]^GATC[CT]
174 AGATCT
XmaCI 472 CCCGGG C^CCGGG
554 CCCGGG
XmaI 472 CCCGGG C^CCGGG
554 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I