Restriction map for
pF1KB0464
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII BbeI BcgI BciVI BglI
BsaHI BsiEI BsiWI BsmBI BsoBI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstUI BstZ17I Bsu36I CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmeI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 489 TCCGGA T^CCGGA
AflII 967 CTTAAG C^TTAAG
AflIII 1469 ACGTGT A^C[GA][CT]GT
AgeI 932 ACCGGT A^CCGGT
AhdI 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1997 GGGCCC GGGCC^C
AspEI 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2076 TGGCCA TGG^CCA
BamHI 529 GGATCC G^GATCC
BbrPI 1468 CACGTG CAC^GTG
BclI 1580 TGATCA T^GATCA
BfrI 967 CTTAAG C^TTAAG
BlpI 961 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 961 GCTCAGC GC^T[ACGT]AGC
BsaAI 1468 CACGTG [CT]AC^GT[GA]
BseAI 489 TCCGGA T^CCGGA
Bsp1407I 2190 TGTACA T^GTACA
BspDI 2893 ATCGAT AT^CGAT
BspEI 489 TCCGGA T^CCGGA
BspHI 1490 TCATGA T^CATGA
BspMI 1620 GCAGGT ACCTGC(4/8)
BsrDI 2768 GCAATG GCAATG(2/0)
BsrGI 2190 TGTACA T^GTACA
BstAPI 21 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1148 TTCGAA TT^CGAA
BstEII 415 GGTAACC G^GT[ACGT]ACC
BstPI 415 GGTAACC G^GT[ACGT]ACC
CelII 961 GCTCAGC GC^T[ACGT]AGC
ClaI 2893 ATCGAT AT^CGAT
DrdI 1390 GACCTGTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2076 TGGCCA [CT]^GGCC[GA]
Eam1105I 2337 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 415 GGTAACC G^GT[ACGT]ACC
FbaI 1580 TGATCA T^GATCA
HaeII 2857 AGCGCC [GA]GCGC^[CT]
MluNI 2076 TGGCCA TGG^CCA
MroI 489 TCCGGA T^CCGGA
MscI 2076 TGGCCA TGG^CCA
NspI 702 GCATGC [GA]CATG^[CT]
NspV 1148 TTCGAA TT^CGAA
PflMI 2810 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 932 ACCGGT A^CCGGT
PmaCI 1468 CACGTG CAC^GTG
PmlI 1468 CACGTG CAC^GTG
PshAI 2143 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1490 TCATGA T^CATGA
SapI 2635 GAAGAGC GCTCTTC(1/4)
SexAI 419 ACCAGGT A^CC[AT]GGT
SfuI 1148 TTCGAA TT^CGAA
SmlI 967 CTTAAG C^T[CT][GA]AG
SphI 702 GCATGC GCATG^C
SspBI 2190 TGTACA T^GTACA
Van91I 2810 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 1473 GTGCAC G^TGCAC
2046 GTGCAC
AlwNI 877 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1397 CAGTCGCTG
ApaLI 1473 GTGCAC G^TGCAC
2046 GTGCAC
AvrII 945 CCTAGG C^CTAGG
2592 CCTAGG
BanI 1174 GGCACC G^G[CT][GA]CC
2155 GGTGCC
BglII 2449 AGATCT A^GATCT
2708 AGATCT
BlnI 945 CCTAGG C^CTAGG
2592 CCTAGG
BsaBI 2074 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2554 GATGAGCATC
BsaI 1125 GGTCTC GGTCTC(1/5)
2019 GGTCTC
BsaWI 489 TCCGGA [AT]^CCGG[AT]
932 ACCGGT
BsgI 1450 CTGCAC GTGCAG(16/14)
2051 CTGCAC
BsmI 21 GCATTC GAATGC(1/-1)
1908 GCATTC
BstXI 719 CCAAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1818 CCAGCCTCTTGG
CfoI 195 GCGC GCG^C
2858 GCGC
EcoNI 562 CCTTCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1165 CCTGTGTCAGG
HgaI 541 GACGC GACGC(5/10)
2401 GACGC
HhaI 195 GCGC GCG^C
2858 GCGC
HinP1I 195 GCGC G^CGC
2858 GCGC
MamI 2074 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2554 GATGAGCATC
MslI 693 CATAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2644 CACTTTGATG
NcoI 2810 CCATGG C^CATGG
2933 CCATGG
XcmI 1212 CCAGGACTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2810 CCATGGAGTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII