################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DrdI DsaI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 391 GACGTC GACGT^C AccI 953 GTCTAC GT^[AC][GT]AC AcyI 391 GACGTC G[GA]^CG[CT]C AgeI 90 ACCGGT A^CCGGT AhdI 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 735 GGATC GGATC(4/5) ApaI 935 GGGCCC GGGCC^C AspEI 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 319 CCCGAG C^[CT]CG[GA]G BalI 929 TGGCCA TGG^CCA BbsI 409 GAAGAC GAAGAC(2/6) BcgI 789 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 606 TGATCA T^GATCA BcnI 630 CCGGG CC^[GC]GG BglI 823 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 409 GAAGAC GAAGAC(2/6) BsaHI 391 GACGTC G[GA]^CG[CT]C BsaWI 90 ACCGGT [AT]^CCGG[AT] BseRI 790 GAGGAG GAGGAG(10/8) BsmAI 238 GAGAC GTCTC(1/5) BsmFI 769 GTCCC GGGAC(10/14) BsoBI 319 CCCGAG C^[CT]CG[GA]G BspMI 476 GCAGGT ACCTGC(4/8) BsrFI 90 ACCGGT [GA]^CCGG[CT] BstAPI 364 GCAAGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 90 ACCGGT [GA]^CCGG[CT] DraI 1036 TTTAAA TTT^AAA DraII 934 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 916 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 929 TGGCCA [CT]^GGCC[GA] Eam1105I 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 934 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRV 620 GATATC GAT^ATC FauI 373 GCGGG CCCGC(4/6) FbaI 606 TGATCA T^GATCA HgaI 589 GACGC GACGC(5/10) Hin1I 391 GACGTC G[GA]^CG[CT]C MluNI 929 TGGCCA TGG^CCA MscI 929 TGGCCA TGG^CCA MslI 739 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 384 CCGCTG C[AC]G^C[GT]G NciI 630 CCGGG CC^[GC]GG PinAI 90 ACCGGT A^CCGGT PleI 677 GAGTC GAGTC(4/5) PshAI 240 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfcI 459 CTACAG C^T[GA][CT]AG SgrAI 89 CACCGGTG C[GA]^CCGG[CT]G TaqI 788 TCGA T^CGA TfiI 692 GATTC G^A[AT]TC TthHB8I 788 TCGA T^CGA XcmI 939 CCAGTGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 399 GTAC GT^AC 480 GTAC AluI 356 AGCT AG^CT 551 AGCT BpmI 542 CTCCAG CTGGAG(16/14) 847 CTCCAG CfoI 58 GCGC GCG^C 912 GCGC DdeI 200 CTAAG C^T[ACGT]AG 503 CTCAG DpnII 607 GATC ^GATC 736 GATC HapII 91 CCGG C^CGG 630 CCGG HhaI 58 GCGC GCG^C 912 GCGC HinP1I 58 GCGC G^CGC 912 GCGC HinfI 677 GAGTC G^A[ACGT]TC 692 GATTC HpaII 91 CCGG C^CGG 630 CCGG MboI 607 GATC ^GATC 736 GATC MboII 409 GAAGA GAAGA(8/7) 568 GAAGA MseI 43 TTAA T^TAA 1037 TTAA MspI 91 CCGG C^CGG 630 CCGG NdeII 607 GATC ^GATC 736 GATC RsaI 399 GTAC GT^AC 480 GTAC Sau3AI 607 GATC ^GATC 736 GATC Tru9I 43 TTAA T^TAA 1037 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AcsI ApoI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeII MaeIII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI