################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FauI FseI FspI Hin1I HpaI KasI KspI MaeII MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 462 GGTACC G^GTACC AccII 1246 CGCG CG^CG AlwI 372 GGATC GGATC(4/5) Aor51HI 806 AGCGCT AGC^GCT Asp718I 462 GGTACC G^GTACC AspHI 412 GAGCAC G[AT]GC[AT]^C AspI 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 291 GGACC G^G[AT]CC BalI 246 TGGCCA TGG^CCA BbsI 704 GAAGAC GAAGAC(2/6) BcnI 78 CCGGG CC^[GC]GG BfaI 16 CTAG C^TAG BpuAI 704 GAAGAC GAAGAC(2/6) BsaBI 204 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 73 GGTCTC GGTCTC(1/5) BseRI 19 GAGGAG GAGGAG(10/8) BsgI 908 CTGCAC GTGCAG(16/14) BsiHKAI 412 GAGCAC G[AT]GC[AT]^C BspMI 47 ACCTGC ACCTGC(4/8) BsrFI 752 GCCGGT [GA]^CCGG[CT] BstUI 1246 CGCG CG^CG BstXI 62 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 372 GGATCT [GA]^GATC[CT] CfoI 807 GCGC GCG^C Cfr10I 752 GCCGGT [GA]^CCGG[CT] DraII 1295 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 1129 CCATGG C^C[GA][CT]GG EaeI 246 TGGCCA [CT]^GGCC[GA] EarI 1228 GAAGAG CTCTTC(1/4) Eco47III 806 AGCGCT AGC^GCT EcoO109I 1295 GGGGCCT [GA]G^G[ACGT]CC[CT] HaeII 806 AGCGCT [GA]GCGC^[CT] HgaI 740 GACGC GACGC(5/10) HhaI 807 GCGC GCG^C HinP1I 807 GCGC G^CGC HindIII 556 AAGCTT A^AGCTT HphI 254 TCACC GGTGA(8/7) KpnI 462 GGTACC GGTAC^C Ksp632I 1228 GAAGAG CTCTTC(1/4) MaeI 16 CTAG C^TAG MamI 204 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 372 GGATCT [GA]^GATC[CT] MluNI 246 TGGCCA TGG^CCA MscI 246 TGGCCA TGG^CCA MspA1I 848 CAGCTG C[AC]G^C[GT]G MvnI 1246 CGCG CG^CG NciI 78 CCGGG CC^[GC]GG NcoI 1129 CCATGG C^CATGG PflFI 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1038 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 848 CAGCTG CAG^CTG SapI 1228 GAAGAGC GCTCTTC(1/4) SfaNI 181 GCATC GCATC(5/9) Tth111I 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 15 TCTAGA T^CTAGA XhoII 372 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 340 GAATTC [GA]^AATT[CT] 987 GAATTC AflIII 912 ACATGT A^C[GA][CT]GT 1167 ACATGT AocI 87 CCTGAGG CC^T[ACGT]AGG 440 CCTCAGG ApoI 340 GAATTC [GA]^AATT[CT] 987 GAATTC BanI 462 GGTACC G^G[CT][GA]CC 931 GGCACC BclI 2 TGATCA T^GATCA 594 TGATCA BmyI 412 GAGCAC G[AGT]GC[ACT]^C 930 GGGCAC BsaJI 782 CCTTGG C^C[ACGT][ACGT]GG 1129 CCATGG BsmFI 958 GGGAC GGGAC(10/14) 1091 GTCCC BsmI 429 GAATGC GAATGC(1/-1) 1286 GAATGC Bsp1286I 412 GAGCAC G[AGT]GC[ACT]^C 930 GGGCAC BspHI 351 TCATGA T^CATGA 561 TCATGA BspLU11I 912 ACATGT A^CATGT 1167 ACATGT Bsu36I 87 CCTGAGG CC^T[ACGT]AGG 440 CCTCAGG Cac8I 743 GCTCGC GC[ACGT]^[ACGT]GC 1247 GCGAGC Eco81I 87 CCTGAGG CC^T[ACGT]AGG 440 CCTCAGG EcoNI 466 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 694 CCTCCAAGAGG EcoRI 340 GAATTC G^AATTC 987 GAATTC EcoT14I 782 CCTTGG C^C[AT][AT]GG 1129 CCATGG FbaI 2 TGATCA T^GATCA 594 TGATCA HapII 78 CCGG C^CGG 753 CCGG HincII 1017 GTTGAC GT[CT]^[GA]AC 1171 GTCAAC HindII 1017 GTTGAC GT[CT]^[GA]AC 1171 GTCAAC HpaII 78 CCGG C^CGG 753 CCGG MspI 78 CCGG C^CGG 753 CCGG NspI 912 ACATGT [GA]CATG^[CT] 1167 ACATGT PstI 49 CTGCAG CTGCA^G 821 CTGCAG RcaI 351 TCATGA T^CATGA 561 TCATGA SfcI 49 CTGCAG C^T[GA][CT]AG 821 CTGCAG SmlI 34 CTCAAG C^T[CT][GA]AG 802 CTCAAG StyI 782 CCTTGG C^C[AT][AT]GG 1129 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI BbvI BpmI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI