################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BanI BbeI BbvCI BclI BfrI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsaI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 716 CGCG CG^CG AocI 621 CCTGAGG CC^T[ACGT]AGG Asp700I 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1381 CTCGAG C^[CT]CG[GA]G AviII 1703 TGCGCA TGC^GCA BalI 251 TGGCCA TGG^CCA BbrPI 1072 CACGTG CAC^GTG BciVI 489 GGATAC GTATCC(6/5) BglII 1212 AGATCT A^GATCT BsaAI 1072 CACGTG [CT]AC^GT[GA] BsgI 528 CTGCAC GTGCAG(16/14) BsoBI 1381 CTCGAG C^[CT]CG[GA]G BsrDI 61 CATTGC GCAATG(2/0) BsrFI 2123 GCCGGC [GA]^CCGG[CT] BssHII 715 GCGCGC G^CGCGC BssSI 322 CACGAG CACGAG(-5/-1) BstUI 716 CGCG CG^CG BstXI 428 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 621 CCTGAGG CC^T[ACGT]AGG Cfr10I 2123 GCCGGC [GA]^CCGG[CT] CpoI 2105 CGGACCG CG^G[AT]CCG DraII 1927 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 1280 GACTACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 824 CCATGG C^C[GA][CT]GG EaeI 251 TGGCCA [CT]^GGCC[GA] Eco81I 621 CCTGAGG CC^T[ACGT]AGG EcoNI 399 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1927 GGGACCC [GA]G^G[ACGT]CC[CT] FspI 1703 TGCGCA TGC^GCA HaeII 242 AGCGCC [GA]GCGC^[CT] HindIII 1109 AAGCTT A^AGCTT MluNI 251 TGGCCA TGG^CCA MscI 251 TGGCCA TGG^CCA MslI 1916 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 716 CGCG CG^CG NaeI 2123 GCCGGC GCC^GGC NcoI 824 CCATGG C^CATGG NgoMIV 2123 GCCGGC G^CCGGC PaeR7I 1381 CTCGAG C^TCGAG PflMI 300 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1072 CACGTG CAC^GTG PmlI 1072 CACGTG CAC^GTG PpuMI 1927 GGGACCC [GA]G^G[AT]CC[CT] PshAI 142 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 2105 CGGACCG CG^G[AT]CCG SacI 760 GAGCTC GAGCT^C StuI 1400 AGGCCT AGG^CCT Van91I 300 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1381 CTCGAG C^TCGAG XmnI 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1071 ACACGT A^C[GA][CT]GT 2219 ACGTGT BanII 760 GAGCTC G[GA]GC[CT]^C 1022 GAGCCC BbsI 1255 GAAGAC GAAGAC(2/6) 1455 GAAGAC BcgI 1050 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1706 GCAGCCGGATCG BfaI 1492 CTAG C^TAG 2255 CTAG BpuAI 1255 GAAGAC GAAGAC(2/6) 1455 GAAGAC BsmI 195 GAATGC GAATGC(1/-1) 1309 GCATTC BsrBI 2268 CCGCTC CCGCTC(-3/-3) 1663 GAGCGG FauI 920 GCGGG CCCGC(4/6) 1251 GCGGG MaeI 1492 CTAG C^TAG 2255 CTAG NspI 893 GCATGC [GA]CATG^[CT] 2177 GCATGC SphI 893 GCATGC GCATG^C 2177 GCATGC Tsp509I 1185 AATT ^AATT 2072 AATT XcmI 254 CCAAAAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 467 CCAAGCAGGTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XhoII