Restriction map for
pF1KE2730
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI Sau96I SbfI
ScaI SfcI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 173 AAATTC [GA]^AATT[CT]
ApoI 173 AAATTC [GA]^AATT[CT]
Asp700I 169 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 631 GAGCTC G[AT]GC[AT]^C
BanII 631 GAGCTC G[GA]GC[CT]^C
BbvI 495 GCAGC GCAGC(8/12)
BclI 41 TGATCA T^GATCA
BsaAI 59 TACGTA [CT]AC^GT[GA]
BseRI 548 GAGGAG GAGGAG(10/8)
BsgI 438 CTGCAC GTGCAG(16/14)
BsiHKAI 631 GAGCTC G[AT]GC[AT]^C
BsmFI 503 GGGAC GGGAC(10/14)
BspMI 130 ACCTGC ACCTGC(4/8)
BssSI 545 CACGAG CACGAG(-5/-1)
DdeI 325 CTAAG C^T[ACGT]AG
DpnII 42 GATC ^GATC
DraI 424 TTTAAA TTT^AAA
EcoT22I 601 ATGCAT ATGCA^T
FbaI 41 TGATCA T^GATCA
Fnu4HI 495 GCAGC GC^[ACGT]GC
HaeIII 525 GGCC GG^CC
HinfI 376 GACTC G^A[ACGT]TC
ItaI 495 GCAGC GC^[ACGT]GC
MboI 42 GATC ^GATC
MslI 476 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 42 GATC ^GATC
NsiI 601 ATGCAT ATGCA^T
PleI 376 GACTC GAGTC(4/5)
SacI 631 GAGCTC GAGCT^C
Sau3AI 42 GATC ^GATC
SexAI 464 ACCAGGT A^CC[AT]GGT
SmlI 634 CTCAAG C^T[CT][GA]AG
SnaBI 59 TACGTA TAC^GTA
SphI 432 GCATGC GCATG^C
TseI 495 GCAGC G^C[AT]GC
XmnI 169 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanI 69 GGCACC G^G[CT][GA]CC
127 GGCACC
BmyI 391 GGGCAC G[AGT]GC[ACT]^C
631 GAGCTC
BpmI 378 CTCCAG CTGGAG(16/14)
457 CTGGAG
Bsp1286I 391 GGGCAC G[AGT]GC[ACT]^C
631 GAGCTC
BstBI 143 TTCGAA TT^CGAA
535 TTCGAA
BstF5I 486 GGATG GGATG(2/0)
521 GGATG
FokI 486 GGATG GGATG(9/13)
521 GGATG
MaeII 60 ACGT A^CGT
395 ACGT
MspA1I 116 CAGCTG C[AC]G^C[GT]G
454 CAGCTG
NlaIV 69 GGCACC GG[ACGT]^[ACGT]CC
127 GGCACC
NspV 143 TTCGAA TT^CGAA
535 TTCGAA
PvuII 116 CAGCTG CAG^CTG
454 CAGCTG
SfaNI 492 GATGC GCATC(5/9)
413 GCATC
SfuI 143 TTCGAA TT^CGAA
535 TTCGAA
Tsp45I 47 GTCAC ^GT[GC]AC
374 GTGAC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI BssKI BstNI Cac8I
EcoRII MaeIII MboII MnlI MseI
MvaI NlaIII NspI RsaI ScrFI
TaqI Tru9I Tsp509I TspRI TthHB8I