Restriction map for
pF1KE2577
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BspEI
BspHI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CpoI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 674 GGTACC G^GTACC
AccI 109 GTAGAC GT^[AC][GT]AC
AccII 753 CGCG CG^CG
AcyI 781 GACGCC G[GA]^CG[CT]C
Asp718I 674 GGTACC G^GTACC
BalI 238 TGGCCA TGG^CCA
BanII 789 GAGCCC G[GA]GC[CT]^C
BlpI 823 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 823 GCTGAGC GC^T[ACGT]AGC
BsaHI 781 GACGCC G[GA]^CG[CT]C
BsgI 715 CTGCAC GTGCAG(16/14)
Bsp1407I 571 TGTACA T^GTACA
BspDI 577 ATCGAT AT^CGAT
BsrDI 179 GCAATG GCAATG(2/0)
BsrGI 571 TGTACA T^GTACA
BstUI 753 CGCG CG^CG
CelII 823 GCTGAGC GC^T[ACGT]AGC
ClaI 577 ATCGAT AT^CGAT
DraI 517 TTTAAA TTT^AAA
EcoNI 813 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 473 GATATC GAT^ATC
FauI 765 GCGGG CCCGC(4/6)
HgaI 781 GACGC GACGC(5/10)
Hin1I 781 GACGCC G[GA]^CG[CT]C
KpnI 674 GGTACC GGTAC^C
MboII 942 GAAGA GAAGA(8/7)
MluNI 238 TGGCCA TGG^CCA
MscI 238 TGGCCA TGG^CCA
MvnI 753 CGCG CG^CG
NaeI 724 GCCGGC GCC^GGC
NgoMIV 724 GCCGGC G^CCGGC
PflMI 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 949 CCCGGG CCC^GGG
SspBI 571 TGTACA T^GTACA
TaqI 578 TCGA T^CGA
TfiI 945 GATTC G^A[AT]TC
Tsp45I 184 GTCAC ^GT[GC]AC
TthHB8I 578 TCGA T^CGA
Van91I 241 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 949 CCCGGG C^CCGGG
XmaI 949 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 76 GAGCAC G[AT]GC[AT]^C
805 GTGCTC
AvaII 270 GGTCC G^G[AT]CC
415 GGTCC
BanI 251 GGCACC G^G[CT][GA]CC
674 GGTACC
BclI 842 TGATCA T^GATCA
926 TGATCA
BglI 204 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
724 GCCGGCAAGGC
BpmI 73 CTGGAG CTGGAG(16/14)
817 CTGGAG
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
805 GTGCTC
BsmAI 364 GAGAC GTCTC(1/5)
690 GAGAC
BsmFI 314 GTCCC GGGAC(10/14)
888 GTCCC
BsmI 170 GCATTC GAATGC(1/-1)
527 GCATTC
BsrFI 587 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
BstAPI 593 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
798 GCAGCTAGTGC
Cfr10I 587 ACCGGC [GA]^CCGG[CT]
724 GCCGGC
DdeI 787 CTGAG C^T[ACGT]AG
824 CTGAG
FbaI 842 TGATCA T^GATCA
926 TGATCA
MseI 29 TTAA T^TAA
518 TTAA
MslI 289 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
879 CATGATGATG
MspA1I 712 CAGCTG C[AC]G^C[GT]G
930 CAGCTG
PleI 312 GAGTC GAGTC(4/5)
485 GAGTC
PstI 51 CTGCAG CTGCA^G
453 CTGCAG
PvuII 712 CAGCTG CAG^CTG
930 CAGCTG
SfaNI 433 GATGC GCATC(5/9)
466 GCATC
Tru9I 29 TTAA T^TAA
518 TTAA
Tsp509I 31 AATT ^AATT
163 AATT
XcmI 62 CCATCTTACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
226 CCAAATGTGTTGTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaI BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI StuI
TseI TspRI