################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) AcsI 634 AAATTC [GA]^AATT[CT] AfaI 428 GTAC GT^AC AluI 330 AGCT AG^CT Aor51HI 66 AGCGCT AGC^GCT ApoI 634 AAATTC [GA]^AATT[CT] AvaI 416 CCCGGG C^[CT]CG[GA]G BalI 587 TGGCCA TGG^CCA BbvCI 81 CCTCAGC CCTCAGC(-5/-2) BfaI 425 CTAG C^TAG BsaAI 736 CACGTA [CT]AC^GT[GA] BsmAI 477 GTCTC GTCTC(1/5) BsoBI 416 CCCGGG C^[CT]CG[GA]G BstF5I 710 CATCC GGATG(2/0) BstNI 441 CCTGG CC^[AT]GG CfoI 67 GCGC GCG^C EaeI 587 TGGCCA [CT]^GGCC[GA] EarI 361 GAAGAG CTCTTC(1/4) Eco47III 66 AGCGCT AGC^GCT EcoRII 441 CCTGG ^CC[AT]GG EcoT14I 399 CCTTGG C^C[AT][AT]GG FokI 710 CATCC GGATG(9/13) HaeII 66 AGCGCT [GA]GCGC^[CT] HapII 417 CCGG C^CGG HgaI 310 GCGTC GACGC(5/10) HhaI 67 GCGC GCG^C HinP1I 67 GCGC G^CGC HpaII 417 CCGG C^CGG Ksp632I 361 GAAGAG CTCTTC(1/4) MaeI 425 CTAG C^TAG MaeIII 346 GTCAC ^GT[ACGT]AC MluNI 587 TGGCCA TGG^CCA MscI 587 TGGCCA TGG^CCA MspA1I 329 CAGCTG C[AC]G^C[GT]G MspI 417 CCGG C^CGG MvaI 441 CCTGG CC^[AT]GG NlaIII 270 CATG CATG^ PvuII 329 CAGCTG CAG^CTG RsaI 428 GTAC GT^AC SmaI 416 CCCGGG CCC^GGG SspI 547 AATATT AAT^ATT StuI 396 AGGCCT AGG^CCT StyI 399 CCTTGG C^C[AT][AT]GG Tsp45I 346 GTCAC ^GT[GC]AC XcmI 431 CCAGTGTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 416 CCCGGG C^CCGGG XmaI 416 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Asp700I 483 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 544 GAAAATATTC AspHI 247 GTGCTC G[AT]GC[AT]^C 409 GTGCTC BcnI 416 CCCGG CC^[GC]GG 417 CCGGG BmyI 247 GTGCTC G[AGT]GC[ACT]^C 409 GTGCTC BpmI 149 CTCCAG CTGGAG(16/14) 91 CTGGAG BsaJI 399 CCTTGG C^C[ACGT][ACGT]GG 416 CCCGGG BsiHKAI 247 GTGCTC G[AT]GC[AT]^C 409 GTGCTC BsiYI 579 CCTCCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 735 CCACGTATGGG BslI 579 CCTCCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 735 CCACGTATGGG BsmFI 453 GGGAC GGGAC(10/14) 41 GTCCC Bsp1286I 247 GTGCTC G[AGT]GC[ACT]^C 409 GTGCTC DdeI 82 CTCAG C^T[ACGT]AG 161 CTGAG DraI 15 TTTAAA TTT^AAA 301 TTTAAA HphI 215 TCACC GGTGA(8/7) 232 TCACC MaeII 407 ACGT A^CGT 737 ACGT MboII 361 GAAGA GAAGA(8/7) 564 TCTTC MwoI 67 GCGCTAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 310 GCGTCCTTTGC NciI 416 CCCGG CC^[GC]GG 417 CCGGG PleI 508 GAGTC GAGTC(4/5) 575 GAGTC TfiI 284 GAATC G^A[AT]TC 488 GATTC XmnI 483 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 544 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BbvI BsrI BssKI Fnu4HI HaeIII HinfI ItaI MnlI MseI ScrFI SmlI Tru9I TseI Tsp509I TspRI