Restriction map for
pF1KE6682
##################################
# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstNI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
HphI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvaI MvnI NarI
NciI NcoI NdeI NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI Sau96I SbfI ScaI
ScrFI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 195 GTAC GT^AC
AlwI 807 GATCC GGATC(4/5)
AspHI 446 GAGCAC G[AT]GC[AT]^C
BbsI 874 GAAGAC GAAGAC(2/6)
BclI 171 TGATCA T^GATCA
BmyI 446 GAGCAC G[AGT]GC[ACT]^C
BpmI 28 CTGGAG CTGGAG(16/14)
BpuAI 874 GAAGAC GAAGAC(2/6)
BseRI 10 GAGGAG GAGGAG(10/8)
BsiHKAI 446 GAGCAC G[AT]GC[AT]^C
BsmFI 556 GGGAC GGGAC(10/14)
Bsp1286I 446 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 194 TGTACA T^GTACA
BspHI 282 TCATGA T^CATGA
BsrFI 34 GCCGGC [GA]^CCGG[CT]
BsrGI 194 TGTACA T^GTACA
BstEII 546 GGTTACC G^GT[ACGT]ACC
BstPI 546 GGTTACC G^GT[ACGT]ACC
BstYI 806 AGATCC [GA]^GATC[CT]
Cfr10I 34 GCCGGC [GA]^CCGG[CT]
EcoO65I 546 GGTTACC G^GT[ACGT]ACC
FbaI 171 TGATCA T^GATCA
HaeIII 33 GGCC GG^CC
HapII 35 CCGG C^CGG
HincII 690 GTTGAC GT[CT]^[GA]AC
HindII 690 GTTGAC GT[CT]^[GA]AC
HindIII 46 AAGCTT A^AGCTT
HpaII 35 CCGG C^CGG
MaeII 124 ACGT A^CGT
MflI 806 AGATCC [GA]^GATC[CT]
MspA1I 776 CAGCTG C[AC]G^C[GT]G
MspI 35 CCGG C^CGG
NaeI 34 GCCGGC GCC^GGC
NgoMIV 34 GCCGGC G^CCGGC
PvuII 776 CAGCTG CAG^CTG
RcaI 282 TCATGA T^CATGA
RsaI 195 GTAC GT^AC
SapI 514 GAAGAGC GCTCTTC(1/4)
SspBI 194 TGTACA T^GTACA
SspI 166 AATATT AAT^ATT
TaqI 60 TCGA T^CGA
TthHB8I 60 TCGA T^CGA
XbaI 468 TCTAGA T^CTAGA
XhoII 806 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BfaI 469 CTAG C^TAG
740 CTAG
BsmAI 15 GAGAC GTCTC(1/5)
667 GAGAC
BstF5I 81 GGATG GGATG(2/0)
663 GGATG
Cac8I 34 GCCGGC GC[ACGT]^[ACGT]GC
48 GCTTGC
DpnII 172 GATC ^GATC
807 GATC
EarI 514 GAAGAG CTCTTC(1/4)
653 GAAGAG
FokI 81 GGATG GGATG(9/13)
663 GGATG
Ksp632I 514 GAAGAG CTCTTC(1/4)
653 GAAGAG
MaeI 469 CTAG C^TAG
740 CTAG
MaeIII 547 GTTAC ^GT[ACGT]AC
576 GTTAC
MboI 172 GATC ^GATC
807 GATC
MwoI 34 GCCGGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
43 GCCAAGCTTGC
NdeII 172 GATC ^GATC
807 GATC
Sau3AI 172 GATC ^GATC
807 GATC
TspRI 181 CAGTG CA[GC]TG[ACGT][ACGT]^
630 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsrI
DdeI Fnu4HI HinfI ItaI MboII
MnlI MseI NlaIII PleI PstI
SfcI TfiI Tru9I TseI Tsp509I