################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MunI NcoI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 235 GAATTC [GA]^AATT[CT] AflIII 1202 ACATGT A^C[GA][CT]GT AgeI 935 ACCGGT A^CCGGT Alw44I 904 GTGCAC G^TGCAC ApaLI 904 GTGCAC G^TGCAC ApoI 235 GAATTC [GA]^AATT[CT] BbsI 1068 GAAGAC GAAGAC(2/6) BcnI 1434 CCCGG CC^[GC]GG BglI 34 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1138 CTGGAG CTGGAG(16/14) BpuAI 1068 GAAGAC GAAGAC(2/6) BsaAI 1150 TACGTG [CT]AC^GT[GA] BseRI 886 GAGGAG GAGGAG(10/8) BspLU11I 1202 ACATGT A^CATGT BsrBI 1083 GAGCGG CCGCTC(-3/-3) BstBI 346 TTCGAA TT^CGAA BstEII 1362 GGTCACC G^GT[ACGT]ACC BstPI 1362 GGTCACC G^GT[ACGT]ACC BstXI 698 CCAGGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1349 GGATCT [GA]^GATC[CT] DraIII 907 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 456 CCGCGG C^C[GA][CT]GG EcoO65I 1362 GGTCACC G^GT[ACGT]ACC EcoRI 235 GAATTC G^AATTC KspI 456 CCGCGG CCGC^GG MflI 1349 GGATCT [GA]^GATC[CT] MseI 1459 TTAA T^TAA NciI 1434 CCCGG CC^[GC]GG NdeI 605 CATATG CA^TATG NspI 1202 ACATGT [GA]CATG^[CT] NspV 346 TTCGAA TT^CGAA PinAI 935 ACCGGT A^CCGGT PleI 1048 GAGTC GAGTC(4/5) PstI 384 CTGCAG CTGCA^G SacII 456 CCGCGG CCGC^GG SfcI 384 CTGCAG C^T[GA][CT]AG SfuI 346 TTCGAA TT^CGAA StuI 485 AGGCCT AGG^CCT Tru9I 1459 TTAA T^TAA XcmI 92 CCAACAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1349 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 202 CGCG CG^CG 457 CGCG AcyI 5 GGCGCC G[GA]^CG[CT]C 1014 GGCGCC AfaI 462 GTAC GT^AC 1344 GTAC AlwNI 523 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 745 CAGGCCCTG ApaI 502 GGGCCC GGGCC^C 1260 GGGCCC BanII 502 GGGCCC G[GA]GC[CT]^C 1260 GGGCCC BbeI 5 GGCGCC GGCGC^C 1014 GGCGCC BsaHI 5 GGCGCC G[GA]^CG[CT]C 1014 GGCGCC BsaWI 869 ACCGGA [AT]^CCGG[AT] 935 ACCGGT BsgI 13 CTGCAC GTGCAG(16/14) 327 CTGCAC BsmI 718 GAATGC GAATGC(1/-1) 491 GCATTC BstUI 202 CGCG CG^CG 457 CGCG EcoT14I 1358 CCAAGG C^C[AT][AT]GG 1415 CCAAGG HaeII 5 GGCGCC [GA]GCGC^[CT] 1014 GGCGCC HgaI 666 GACGC GACGC(5/10) 1062 GACGC Hin1I 5 GGCGCC G[GA]^CG[CT]C 1014 GGCGCC KasI 5 GGCGCC G^GCGCC 1014 GGCGCC MvnI 202 CGCG CG^CG 457 CGCG NaeI 1112 GCCGGC GCC^GGC 1213 GCCGGC NarI 5 GGCGCC GG^CGCC 1014 GGCGCC NgoMIV 1112 GCCGGC G^CCGGC 1213 GCCGGC PvuII 1164 CAGCTG CAG^CTG 1311 CAGCTG RsaI 462 GTAC GT^AC 1344 GTAC SfoI 5 GGCGCC GGC^GCC 1014 GGCGCC StyI 1358 CCAAGG C^C[AT][AT]GG 1415 CCAAGG TfiI 344 GATTC G^A[AT]TC 848 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AluI AlwI Asp700I AspHI AvaI AvaII BanI BbvI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XmnI