Restriction map for
pF1KE2553
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tru9I Tth111I
Van91I XcmI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 560 AAATTC [GA]^AATT[CT]
AflIII 350 ACGCGT A^C[GA][CT]GT
AlwI 432 GATCC GGATC(4/5)
AocI 326 CCTCAGG CC^T[ACGT]AGG
ApoI 560 AAATTC [GA]^AATT[CT]
Asp700I 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 255 GGTCC G^G[AT]CC
BalI 617 TGGCCA TGG^CCA
BfaI 55 CTAG C^TAG
BseRI 234 CTCCTC GAGGAG(10/8)
BsiEI 417 CGACCG CG[GA][CT]^CG
BsmAI 276 GTCTC GTCTC(1/5)
BsmI 524 GAATGC GAATGC(1/-1)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BstNI 181 CCTGG CC^[AT]GG
Bsu36I 326 CCTCAGG CC^T[ACGT]AGG
Cac8I 46 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
Cfr13I 255 GGTCC G^G[ACGT]CC
EaeI 617 TGGCCA [CT]^GGCC[GA]
EarI 73 GAAGAG CTCTTC(1/4)
Eco81I 326 CCTCAGG CC^T[ACGT]AGG
EcoRII 181 CCTGG ^CC[AT]GG
FauI 314 GCGGG CCCGC(4/6)
HindIII 141 AAGCTT A^AGCTT
Ksp632I 73 GAAGAG CTCTTC(1/4)
MaeI 55 CTAG C^TAG
MluI 350 ACGCGT A^CGCGT
MluNI 617 TGGCCA TGG^CCA
MscI 617 TGGCCA TGG^CCA
MslI 349 CACGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 423 CAGCTG C[AC]G^C[GT]G
MvaI 181 CCTGG CC^[AT]GG
NaeI 46 GCCGGC GCC^GGC
NgoMIV 46 GCCGGC G^CCGGC
PaeR7I 40 CTCGAG C^TCGAG
PstI 599 CTGCAG CTGCA^G
PvuII 423 CAGCTG CAG^CTG
Sau96I 255 GGTCC G^G[ACGT]CC
SfcI 599 CTGCAG C^T[GA][CT]AG
SmaI 674 CCCGGG CCC^GGG
Tsp509I 561 AATT ^AATT
XbaI 54 TCTAGA T^CTAGA
XhoI 40 CTCGAG C^TCGAG
XmaCI 674 CCCGGG C^CCGGG
XmaI 674 CCCGGG C^CCGGG
XmnI 645 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 351 CGCG CG^CG
507 CGCG
AvaI 40 CTCGAG C^[CT]CG[GA]G
674 CCCGGG
BanII 60 GAGCCC G[GA]GC[CT]^C
228 GAGCCC
BglII 532 AGATCT A^GATCT
603 AGATCT
BmyI 60 GAGCCC G[AGT]GC[ACT]^C
228 GAGCCC
BsiYI 36 CCCACTCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCCCTCCTCGG
BslI 36 CCCACTCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
231 CCCCTCCTCGG
BsmFI 317 GGGAC GGGAC(10/14)
256 GTCCC
BsoBI 40 CTCGAG C^[CT]CG[GA]G
674 CCCGGG
Bsp1286I 60 GAGCCC G[AGT]GC[ACT]^C
228 GAGCCC
BstUI 351 CGCG CG^CG
507 CGCG
HphI 240 GGTGA GGTGA(8/7)
217 TCACC
MvnI 351 CGCG CG^CG
507 CGCG
NlaIV 255 GGTCCC GG[ACGT]^[ACGT]CC
751 GGAACC
SmlI 40 CTCGAG C^T[CT][GA]AG
394 CTCAAG
StuI 323 AGGCCT AGG^CCT
635 AGGCCT
Tsp45I 241 GTGAC ^GT[GC]AC
498 GTCAC
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
756 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsrI BssKI BstF5I BstYI DdeI
DpnII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MflI MnlI MspI MwoI
NciI NdeII NlaIII PleI Sau3AI
ScrFI TaqI TfiI TseI TthHB8I
XhoII