Restriction map for
pF1KE5454
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaII KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 992 CGCG CG^CG
AciI 396 CCGC CCGC(-3/-1)
AcsI 599 AAATTC [GA]^AATT[CT]
AflIII 99 ACATGT A^C[GA][CT]GT
ApoI 599 AAATTC [GA]^AATT[CT]
AspHI 559 GAGCTC G[AT]GC[AT]^C
AvaI 1054 CCCGAG C^[CT]CG[GA]G
AvaII 777 GGACC G^G[AT]CC
BanI 906 GGCACC G^G[CT][GA]CC
BbsI 34 GTCTTC GAAGAC(2/6)
BcgI 1034 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 288 TGATCA T^GATCA
BpmI 978 CTGGAG CTGGAG(16/14)
BpuAI 34 GTCTTC GAAGAC(2/6)
BsaI 541 GGTCTC GGTCTC(1/5)
BseRI 867 CTCCTC GAGGAG(10/8)
BsiHKAI 559 GAGCTC G[AT]GC[AT]^C
BsmI 166 GCATTC GAATGC(1/-1)
BsoBI 1054 CCCGAG C^[CT]CG[GA]G
BspLU11I 99 ACATGT A^CATGT
BspMI 612 ACCTGC ACCTGC(4/8)
BstAPI 1009 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 992 CGCG CG^CG
DraI 714 TTTAAA TTT^AAA
DraIII 406 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 459 CTCTTC CTCTTC(1/4)
FauI 395 CCCGC CCCGC(4/6)
FbaI 288 TGATCA T^GATCA
HincII 820 GTTAAC GT[CT]^[GA]AC
HindII 820 GTTAAC GT[CT]^[GA]AC
HpaI 820 GTTAAC GTT^AAC
HphI 999 TCACC GGTGA(8/7)
Ksp632I 459 CTCTTC CTCTTC(1/4)
MslI 516 CACGGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 992 CGCG CG^CG
MwoI 1009 GCAACCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 99 ACATGT [GA]CATG^[CT]
PflMI 693 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 593 GACTC GAGTC(4/5)
PvuII 292 CAGCTG CAG^CTG
SacI 559 GAGCTC GAGCT^C
SmlI 86 CTCAAG C^T[CT][GA]AG
SspI 840 AATATT AAT^ATT
Tsp45I 514 GTCAC ^GT[GC]AC
Van91I 693 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 476 CCATTTTAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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BanII 559 GAGCTC G[GA]GC[CT]^C
799 GAGCCC
BbvCI 1045 CCTCAGC CCTCAGC(-5/-2)
154 GCTGAGG
BbvI 703 GCAGC GCAGC(8/12)
310 GCTGC
BmyI 559 GAGCTC G[AGT]GC[ACT]^C
799 GAGCCC
BsaJI 972 CCCAGG C^C[ACGT][ACGT]GG
1022 CCTGGG
BsgI 352 GTGCAG GTGCAG(16/14)
490 GTGCAG
Bsp1286I 559 GAGCTC G[AGT]GC[ACT]^C
799 GAGCCC
BsrI 538 ACTGG ACTGG(1/-1)
31 CCAGT
Cac8I 706 GCAGGC GC[ACGT]^[ACGT]GC
903 GCAGGC
CfoI 42 GCGC GCG^C
991 GCGC
DpnII 289 GATC ^GATC
995 GATC
Fnu4HI 310 GCTGC GC^[ACGT]GC
703 GCAGC
HaeIII 142 GGCC GG^CC
323 GGCC
HhaI 42 GCGC GCG^C
991 GCGC
HinP1I 42 GCGC G^CGC
991 GCGC
ItaI 310 GCTGC GC^[ACGT]GC
703 GCAGC
MaeIII 514 GTCAC ^GT[ACGT]AC
609 GTTAC
MboI 289 GATC ^GATC
995 GATC
MspA1I 292 CAGCTG C[AC]G^C[GT]G
396 CCGCTG
NdeII 289 GATC ^GATC
995 GATC
NlaIV 91 GGAACC GG[ACGT]^[ACGT]CC
906 GGCACC
Sau3AI 289 GATC ^GATC
995 GATC
SexAI 407 ACCAGGT A^CC[AT]GGT
485 ACCTGGT
SfaNI 628 GATGC GCATC(5/9)
271 GCATC
TaqI 591 TCGA T^CGA
889 TCGA
TseI 310 GCTGC G^C[AT]GC
703 GCAGC
Tsp509I 189 AATT ^AATT
600 AATT
TthHB8I 591 TCGA T^CGA
889 TCGA
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsiYI BslI BsmAI BsrDI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII FokI HinfI MboII MnlI
MseI MvaI NlaIII Sau96I ScrFI
TfiI Tru9I TspRI