Restriction map for
pF1KB5827
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbrPI BclI BfrI BglII
BsaBI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EarI Eco47III
Eco81I EcoRI FbaI FseI FspI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MseI MunI NcoI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SexAI SfiI SfuI SmaI
SnaBI SpeI SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1448 CGCG CG^CG
AhdI 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1312 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 112 CTCGGG C^[CT]CG[GA]G
AvrII 711 CCTAGG C^CTAGG
BalI 434 TGGCCA TGG^CCA
BcgI 104 GCAGGGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 665 GTATCC GTATCC(6/5)
BcnI 525 CCGGG CC^[GC]GG
BlnI 711 CCTAGG C^CTAGG
BsaAI 533 TACGTG [CT]AC^GT[GA]
BsaI 572 GGTCTC GGTCTC(1/5)
BsaWI 1502 TCCGGT [AT]^CCGG[AT]
BsiEI 81 CGGCCG CG[GA][CT]^CG
BsoBI 112 CTCGGG C^[CT]CG[GA]G
BstUI 1448 CGCG CG^CG
BstXI 412 CCATCCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1796 AGATCC [GA]^GATC[CT]
EagI 81 CGGCCG C^GGCCG
Eam1105I 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 81 CGGCCG C^GGCCG
Eco52I 81 CGGCCG C^GGCCG
EcoRV 2055 GATATC GAT^ATC
EcoT22I 910 ATGCAT ATGCA^T
HindIII 1330 AAGCTT A^AGCTT
MaeII 534 ACGT A^CGT
MflI 1796 AGATCC [GA]^GATC[CT]
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MvnI 1448 CGCG CG^CG
NciI 525 CCGGG CC^[GC]GG
NdeI 417 CATATG CA^TATG
NheI 1570 GCTAGC G^CTAGC
NsiI 910 ATGCAT ATGCA^T
NspI 1936 GCATGC [GA]CATG^[CT]
PleI 640 GAGTC GAGTC(4/5)
PshAI 592 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1011 CCTGCAGG CCTGCA^GG
SphI 1936 GCATGC GCATG^C
Sse8387I 1011 CCTGCAGG CCTGCA^GG
StuI 1523 AGGCCT AGG^CCT
TaqI 1548 TCGA T^CGA
TthHB8I 1548 TCGA T^CGA
XhoII 1796 AGATCC [GA]^GATC[CT]
XmnI 1312 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 67 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
AfaI 1218 GTAC GT^AC
1294 GTAC
ApaI 779 GGGCCC GGGCC^C
1913 GGGCCC
BbeI 67 GGCGCC GGCGC^C
73 GGCGCC
BbsI 1968 GAAGAC GAAGAC(2/6)
1471 GTCTTC
BbvCI 732 GCTGAGG CCTCAGC(-5/-2)
1350 GCTGAGG
BglI 83 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1878 GCCACTGTGGC
BlpI 483 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
Bpu1102I 483 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
BpuAI 1968 GAAGAC GAAGAC(2/6)
1471 GTCTTC
BsaHI 67 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
BspHI 200 TCATGA T^CATGA
498 TCATGA
BsrBI 1642 CCGCTC CCGCTC(-3/-3)
181 GAGCGG
BstAPI 793 GCAGGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1887 GCACCTCTTGC
BstEII 652 GGTTACC G^GT[ACGT]ACC
1950 GGTGACC
BstPI 652 GGTTACC G^GT[ACGT]ACC
1950 GGTGACC
CelII 483 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
DsaI 701 CCACGG C^C[GA][CT]GG
1667 CCGTGG
EcoO65I 652 GGTTACC G^GT[ACGT]ACC
1950 GGTGACC
FauI 16 GCGGG CCCGC(4/6)
183 GCGGG
HgaI 1446 GACGC GACGC(5/10)
1202 GCGTC
Hin1I 67 GGCGCC G[GA]^CG[CT]C
73 GGCGCC
KasI 67 GGCGCC G^GCGCC
73 GGCGCC
NaeI 70 GCCGGC GCC^GGC
76 GCCGGC
NarI 67 GGCGCC GG^CGCC
73 GGCGCC
NgoMIV 70 GCCGGC G^CCGGC
76 GCCGGC
PflMI 770 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCAGCGTCTGG
PstI 367 CTGCAG CTGCA^G
1012 CTGCAG
RcaI 200 TCATGA T^CATGA
498 TCATGA
RsaI 1218 GTAC GT^AC
1294 GTAC
SacI 580 GAGCTC GAGCT^C
1150 GAGCTC
SfoI 67 GGCGCC GGC^GCC
73 GGCGCC
SgrAI 69 CGCCGGCG C[GA]^CCGG[CT]G
75 CGCCGGCG
SmlI 1327 CTCAAG C^T[CT][GA]AG
1897 CTTGAG
Van91I 770 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1199 CCAGCGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanI BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PpuMI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI XcmI