Restriction map for
pF1KE5816
##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BciVI
BclI BfrI BlnI BsaBI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I FbaI FseI HindIII
HpaI KpnI KspI MamI MluI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 176 TCCGGA T^CCGGA
AflIII 92 ACATGT A^C[GA][CT]GT
Aor51HI 134 AGCGCT AGC^GCT
ApaI 1696 GGGCCC GGGCC^C
AviII 356 TGCGCA TGC^GCA
BalI 335 TGGCCA TGG^CCA
BamHI 81 GGATCC G^GATCC
BbeI 559 GGCGCC GGCGC^C
BbrPI 1516 CACGTG CAC^GTG
BbvCI 48 GCTGAGG CCTCAGC(-5/-2)
BcgI 447 CGACTTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1486 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1706 AGATCT A^GATCT
BsaWI 176 TCCGGA [AT]^CCGG[AT]
BseAI 176 TCCGGA T^CCGGA
BspEI 176 TCCGGA T^CCGGA
BspLU11I 92 ACATGT A^CATGT
BsrBI 571 GAGCGG CCGCTC(-3/-3)
BsrFI 35 GCCGGC [GA]^CCGG[CT]
BstXI 1439 CCATCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 35 GCCGGC [GA]^CCGG[CT]
DraII 1696 GGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 853 GAAGAG CTCTTC(1/4)
Eco47III 134 AGCGCT AGC^GCT
EcoNI 1574 CCTACGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1696 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 1976 CCATGG C^C[AT][AT]GG
EcoT22I 1214 ATGCAT ATGCA^T
FspI 356 TGCGCA TGC^GCA
HincII 1031 GTCAAC GT[CT]^[GA]AC
HindII 1031 GTCAAC GT[CT]^[GA]AC
KasI 559 GGCGCC G^GCGCC
Ksp632I 853 GAAGAG CTCTTC(1/4)
MfeI 991 CAATTG C^AATTG
MluNI 335 TGGCCA TGG^CCA
MroI 176 TCCGGA T^CCGGA
MscI 335 TGGCCA TGG^CCA
MunI 991 CAATTG C^AATTG
NaeI 35 GCCGGC GCC^GGC
NarI 559 GGCGCC GG^CGCC
NcoI 1976 CCATGG C^CATGG
NgoMIV 35 GCCGGC G^CCGGC
NsiI 1214 ATGCAT ATGCA^T
PmaCI 1516 CACGTG CAC^GTG
PmlI 1516 CACGTG CAC^GTG
SacI 730 GAGCTC GAGCT^C
SfoI 559 GGCGCC GGC^GCC
SphI 2240 GCATGC GCATG^C
StuI 1080 AGGCCT AGG^CCT
StyI 1976 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 120 GACGCC G[GA]^CG[CT]C
559 GGCGCC
Alw44I 538 GTGCAC G^TGCAC
606 GTGCAC
AlwNI 403 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
430 CAGGAGCTG
AocI 1140 CCTGAGG CC^T[ACGT]AGG
2559 CCTGAGG
ApaLI 538 GTGCAC G^TGCAC
606 GTGCAC
AspI 709 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2404 GACGAAGTC
AvaI 124 CCCGAG C^[CT]CG[GA]G
2069 CCCGAG
BbsI 706 GAAGAC GAAGAC(2/6)
2262 GAAGAC
BpuAI 706 GAAGAC GAAGAC(2/6)
2262 GAAGAC
BsaHI 120 GACGCC G[GA]^CG[CT]C
559 GGCGCC
BsmI 1212 GAATGC GAATGC(1/-1)
1360 GAATGC
BsoBI 124 CCCGAG C^[CT]CG[GA]G
2069 CCCGAG
BspMI 1415 ACCTGC ACCTGC(4/8)
2115 ACCTGC
BstYI 81 GGATCC [GA]^GATC[CT]
1706 AGATCT
Bsu36I 1140 CCTGAGG CC^T[ACGT]AGG
2559 CCTGAGG
DraI 870 TTTAAA TTT^AAA
1858 TTTAAA
EaeI 251 TGGCCG [CT]^GGCC[GA]
335 TGGCCA
Eco81I 1140 CCTGAGG CC^T[ACGT]AGG
2559 CCTGAGG
EcoRI 2247 GAATTC G^AATTC
2289 GAATTC
EcoRV 1732 GATATC GAT^ATC
2443 GATATC
Hin1I 120 GACGCC G[GA]^CG[CT]C
559 GGCGCC
MaeIII 277 GTCAC ^GT[ACGT]AC
1972 GTCAC
MflI 81 GGATCC [GA]^GATC[CT]
1706 AGATCT
PflFI 709 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2404 GACGAAGTC
PstI 244 CTGCAG CTGCA^G
1702 CTGCAG
PvuII 247 CAGCTG CAG^CTG
1498 CAGCTG
SfcI 244 CTGCAG C^T[GA][CT]AG
1702 CTGCAG
TaqI 569 TCGA T^CGA
1934 TCGA
TfiI 2171 GAATC G^A[AT]TC
2511 GATTC
Tsp45I 277 GTCAC ^GT[GC]AC
1972 GTCAC
TspRI 517 CACTG CA[GC]TG[ACGT][ACGT]^
1904 CAGTG
Tth111I 709 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2404 GACGAAGTC
TthHB8I 569 TCGA T^CGA
1934 TCGA
XhoII 81 GGATCC [GA]^GATC[CT]
1706 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI Tru9I TseI Tsp509I