Restriction map for
pF1KE2484
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AclI AflII AocI AscI
AseI AsnI Asp718I AspI AvrII
BfrI BlnI BsiWI BsmI BspDI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI Eco81I EcoRI
EcoRV HindIII HpaI KpnI MluI
NdeI NotI NruI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SfuI SgrAI SnaBI
SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 5958 GACGTC GACGT^C
AccIII 6992 TCCGGA T^CCGGA
AgeI 4379 ACCGGT A^CCGGT
Asp700I 4308 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3838 CACGTG CAC^GTG
BseAI 6992 TCCGGA T^CCGGA
Bsp1407I 1322 TGTACA T^GTACA
BspEI 6992 TCCGGA T^CCGGA
BsrGI 1322 TGTACA T^GTACA
BstEII 4875 GGTCACC G^GT[ACGT]ACC
BstPI 4875 GGTCACC G^GT[ACGT]ACC
DraIII 1477 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 4875 GGTCACC G^GT[ACGT]ACC
EcoT22I 2650 ATGCAT ATGCA^T
FseI 88 GGCCGGCC GGCCGG^CC
KspI 208 CCGCGG CCGC^GG
MfeI 5406 CAATTG C^AATTG
MroI 6992 TCCGGA T^CCGGA
MunI 5406 CAATTG C^AATTG
NheI 1270 GCTAGC G^CTAGC
NsiI 2650 ATGCAT ATGCA^T
PaeR7I 3745 CTCGAG C^TCGAG
PinAI 4379 ACCGGT A^CCGGT
PmaCI 3838 CACGTG CAC^GTG
PmlI 3838 CACGTG CAC^GTG
SacII 208 CCGCGG CCGC^GG
SbfI 348 CCTGCAGG CCTGCA^GG
SpeI 1818 ACTAGT A^CTAGT
Sse8387I 348 CCTGCAGG CCTGCA^GG
SspBI 1322 TGTACA T^GTACA
XhoI 3745 CTCGAG C^TCGAG
XmnI 4308 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 1069 GTCGAC GT^[AC][GT]AC
2965 GTCGAC
AcsI 735 GAATTT [GA]^AATT[CT]
2712 GAATTT
AflIII 4589 ACATGT A^C[GA][CT]GT
6155 ACATGT
AhdI 3754 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5446 GACCAGGAGTC
ApoI 735 GAATTT [GA]^AATT[CT]
2712 GAATTT
AspEI 3754 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5446 GACCAGGAGTC
AviII 590 TGCGCA TGC^GCA
5141 TGCGCA
BbvCI 5631 CCTCAGC CCTCAGC(-5/-2)
4128 GCTGAGG
BclI 1157 TGATCA T^GATCA
4172 TGATCA
BsaAI 2857 TACGTG [CT]AC^GT[GA]
3838 CACGTG
BsaBI 1032 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2250 GATCGCCATC
BsgI 5725 CTGCAC GTGCAG(16/14)
4291 GTGCAG
BsmBI 1798 GAGACG CGTCTC(1/5)
4207 GAGACG
BspLU11I 4589 ACATGT A^CATGT
6155 ACATGT
BssSI 2293 CACGAG CACGAG(-5/-1)
3241 CACGAG
DrdI 3835 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4209 GACGCTGATGTC
Eam1105I 3754 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5446 GACCAGGAGTC
FbaI 1157 TGATCA T^GATCA
4172 TGATCA
FspI 590 TGCGCA TGC^GCA
5141 TGCGCA
MamI 1032 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2250 GATCGCCATC
SalI 1069 GTCGAC G^TCGAC
2965 GTCGAC
SexAI 1316 ACCTGGT A^CC[AT]GGT
1478 ACCTGGT
SfiI 1539 GGCCCCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5863 GGCCCAGGGGGCC
StuI 353 AGGCCT AGG^CCT
908 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvI BcgI BciVI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI