Restriction map for
pF1KE5152
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BbvI BciVI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI Fnu4HI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI ItaI KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MroI
MseI MspA1I MunI MvnI NaeI
NarI NciI NdeI NdeII NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
TseI Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 119 GCGG CCGC(-3/-1)
BalI 195 TGGCCA TGG^CCA
BanII 121 GGGCTC G[GA]GC[CT]^C
BcgI 155 GCATCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 67 CTAG C^TAG
BlpI 248 GCTCAGC GC^T[ACGT]AGC
BmyI 121 GGGCTC G[AGT]GC[ACT]^C
BpmI 109 CTCCAG CTGGAG(16/14)
Bpu1102I 248 GCTCAGC GC^T[ACGT]AGC
BsaWI 205 TCCGGT [AT]^CCGG[AT]
BsiYI 213 CCATCCAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 213 CCATCCAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 223 GAGAC GTCTC(1/5)
Bsp1286I 121 GGGCTC G[AGT]GC[ACT]^C
BspHI 167 TCATGA T^CATGA
BsrI 98 ACTGG ACTGG(1/-1)
CelII 248 GCTCAGC GC^T[ACGT]AGC
Cfr13I 18 GGGCC G^G[ACGT]CC
DdeI 249 CTCAG C^T[ACGT]AG
DsaI 149 CCATGG C^C[GA][CT]GG
EaeI 195 TGGCCA [CT]^GGCC[GA]
EcoT14I 149 CCATGG C^C[AT][AT]GG
FauI 119 GCGGG CCCGC(4/6)
HapII 206 CCGG C^CGG
HinfI 225 GACTC G^A[ACGT]TC
HpaII 206 CCGG C^CGG
MaeI 67 CTAG C^TAG
MboII 70 GAAGA GAAGA(8/7)
MluNI 195 TGGCCA TGG^CCA
MscI 195 TGGCCA TGG^CCA
MslI 162 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 206 CCGG C^CGG
NcoI 149 CCATGG C^CATGG
PleI 225 GACTC GAGTC(4/5)
RcaI 167 TCATGA T^CATGA
Sau96I 18 GGGCC G^G[ACGT]CC
StyI 149 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BstEII 9 GGTGACC G^GT[ACGT]ACC
208 GGTCACC
BstF5I 214 CATCC GGATG(2/0)
54 GGATG
BstPI 9 GGTGACC G^GT[ACGT]ACC
208 GGTCACC
CfoI 131 GCGC GCG^C
146 GCGC
EcoO65I 9 GGTGACC G^GT[ACGT]ACC
208 GGTCACC
FokI 214 CATCC GGATG(9/13)
54 GGATG
HaeIII 19 GGCC GG^CC
196 GGCC
HhaI 131 GCGC GCG^C
146 GCGC
HinP1I 131 GCGC G^CGC
146 GCGC
MaeIII 10 GTGAC ^GT[ACGT]AC
209 GTCAC
MnlI 108 CCTC CCTC(7/6)
232 CCTC
MwoI 146 GCGCCATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
188 GCAAGTTTGGC
NlaIII 150 CATG CATG^
168 CATG
SfaNI 55 GATGC GCATC(5/9)
155 GCATC
Tsp45I 10 GTGAC ^GT[GC]AC
209 GTCAC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BsaJI BssKI BstNI Cac8I EcoRII
HphI MvaI ScrFI