Restriction map for
pF1KE5553
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BanI BbeI
BbvCI BcgI BciVI BcnI BfaI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HapII HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeI MamI
MluI MroI MspI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 874 GTCTAC GT^[AC][GT]AC
AcsI 612 AAATTC [GA]^AATT[CT]
AcyI 221 GGCGTC G[GA]^CG[CT]C
AflII 1042 CTTAAG C^TTAAG
AflIII 29 ACGTGT A^C[GA][CT]GT
ApoI 612 AAATTC [GA]^AATT[CT]
AspHI 1192 GAGCTC G[AT]GC[AT]^C
BamHI 752 GGATCC G^GATCC
BbrPI 114 CACGTG CAC^GTG
BclI 1115 TGATCA T^GATCA
BfrI 1042 CTTAAG C^TTAAG
BglII 170 AGATCT A^GATCT
BsaHI 221 GGCGTC G[GA]^CG[CT]C
BsgI 735 GTGCAG GTGCAG(16/14)
BsiHKAI 1192 GAGCTC G[AT]GC[AT]^C
BsiYI 579 CCCAATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 579 CCCAATTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1257 GAGAC GTCTC(1/5)
BsmI 1245 GAATGC GAATGC(1/-1)
BspMI 362 ACCTGC ACCTGC(4/8)
BstAPI 469 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 103 GACAAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 997 CTCTTC CTCTTC(1/4)
FauI 369 CCCGC CCCGC(4/6)
FbaI 1115 TGATCA T^GATCA
HgaI 222 GCGTC GACGC(5/10)
Hin1I 221 GGCGTC G[GA]^CG[CT]C
Ksp632I 997 CTCTTC CTCTTC(1/4)
MfeI 581 CAATTG C^AATTG
MunI 581 CAATTG C^AATTG
PleI 289 GAGTC GAGTC(4/5)
PmaCI 114 CACGTG CAC^GTG
PmlI 114 CACGTG CAC^GTG
PvuII 389 CAGCTG CAG^CTG
SacI 1192 GAGCTC GAGCT^C
ScaI 593 AGTACT AGT^ACT
SmlI 1042 CTTAAG C^T[CT][GA]AG
SspI 1057 AATATT AAT^ATT
XcmI 5 CCATCGCTCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 162 CGCG CG^CG
479 CGCG
BalI 2 TGGCCA TGG^CCA
17 TGGCCA
BbsI 600 GAAGAC GAAGAC(2/6)
903 GAAGAC
BpmI 88 CTGGAG CTGGAG(16/14)
1051 CTGGAG
BpuAI 600 GAAGAC GAAGAC(2/6)
903 GAAGAC
BsaAI 28 TACGTG [CT]AC^GT[GA]
114 CACGTG
BsrDI 120 CATTGC GCAATG(2/0)
801 CATTGC
BstF5I 333 CATCC GGATG(2/0)
1125 GGATG
BstUI 162 CGCG CG^CG
479 CGCG
BstYI 170 AGATCT [GA]^GATC[CT]
752 GGATCC
CfoI 163 GCGC GCG^C
478 GCGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
17 TGGCCA
EcoT14I 1107 CCTTGG C^C[AT][AT]GG
1197 CCAAGG
FokI 333 CATCC GGATG(9/13)
1125 GGATG
HhaI 163 GCGC GCG^C
478 GCGC
HinP1I 163 GCGC G^CGC
478 GCGC
MaeII 29 ACGT A^CGT
115 ACGT
MflI 170 AGATCT [GA]^GATC[CT]
752 GGATCC
MluNI 2 TGGCCA TGG^CCA
17 TGGCCA
MscI 2 TGGCCA TGG^CCA
17 TGGCCA
MspA1I 370 CCGCTG C[AC]G^C[GT]G
389 CAGCTG
MvnI 162 CGCG CG^CG
479 CGCG
PstI 280 CTGCAG CTGCA^G
1154 CTGCAG
SfaNI 740 GCATC GCATC(5/9)
812 GCATC
SfcI 280 CTGCAG C^T[GA][CT]AG
1154 CTGCAG
StyI 1107 CCTTGG C^C[AT][AT]GG
1197 CCAAGG
TaqI 175 TCGA T^CGA
1082 TCGA
TfiI 241 GATTC G^A[AT]TC
1267 GATTC
Tsp45I 112 GTCAC ^GT[GC]AC
482 GTGAC
TthHB8I 175 TCGA T^CGA
1082 TCGA
XhoII 170 AGATCT [GA]^GATC[CT]
752 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BseRI Bsp1286I
BsrI BssKI BstNI Cac8I DdeI
DpnII DsaI EcoRII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI Tru9I TseI Tsp509I TspRI