Restriction map for
pF1KE2399
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# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaLI AscI AviII BbrPI BfrI
BsaAI BsiWI BsmBI BspDI ClaI
CpoI FseI FspI MfeI MluI
MunI NheI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 2329 TCCGGA T^CCGGA
AcsI 3606 GAATTC [GA]^AATT[CT]
AflIII 5738 ACATGT A^C[GA][CT]GT
AhdI 5737 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3606 GAATTC [GA]^AATT[CT]
AseI 2800 ATTAAT AT^TAAT
AsnI 2800 ATTAAT AT^TAAT
Asp700I 5296 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5737 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4384 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 325 CCTAGG C^CTAGG
BglII 5177 AGATCT A^GATCT
BlnI 325 CCTAGG C^CTAGG
BseAI 2329 TCCGGA T^CCGGA
BsmI 3310 GAATGC GAATGC(1/-1)
Bsp1407I 4163 TGTACA T^GTACA
BspEI 2329 TCCGGA T^CCGGA
BspHI 5102 TCATGA T^CATGA
BspLU11I 5738 ACATGT A^CATGT
BsrGI 4163 TGTACA T^GTACA
BssHII 6707 GCGCGC G^CGCGC
Bst1107I 4279 GTATAC GTA^TAC
BstBI 5963 TTCGAA TT^CGAA
BstZ17I 4279 GTATAC GTA^TAC
DraI 5190 TTTAAA TTT^AAA
DrdI 3435 GACTCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5737 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3606 GAATTC G^AATTC
EcoRV 4912 GATATC GAT^ATC
HpaI 2922 GTTAAC GTT^AAC
MaeII 898 ACGT A^CGT
MroI 2329 TCCGGA T^CCGGA
NspV 5963 TTCGAA TT^CGAA
PflFI 4384 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2800 ATTAAT AT^TAAT
RcaI 5102 TCATGA T^CATGA
SalI 1614 GTCGAC G^TCGAC
SfuI 5963 TTCGAA TT^CGAA
SpeI 6676 ACTAGT A^CTAGT
SphI 4730 GCATGC GCATG^C
SspBI 4163 TGTACA T^GTACA
SspI 5153 AATATT AAT^ATT
Tth111I 4384 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5296 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 1247 GGTACC G^GTACC
1286 GGTACC
AcyI 399 GGCGCC G[GA]^CG[CT]C
970 GGCGCC
Asp718I 1247 GGTACC G^GTACC
1286 GGTACC
BbeI 399 GGCGCC GGCGC^C
970 GGCGCC
BcgI 1292 CGATGACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3920 CGAATCTCATGC
BciVI 1734 GTATCC GTATCC(6/5)
4753 GTATCC
BclI 2819 TGATCA T^GATCA
5414 TGATCA
BsaBI 3410 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5172 GATTGAGATC
BsaHI 399 GGCGCC G[GA]^CG[CT]C
970 GGCGCC
BsrBI 1117 CCGCTC CCGCTC(-3/-3)
555 GAGCGG
BssSI 4629 CTCGTG CACGAG(-5/-1)
6545 CTCGTG
BstEII 3769 GGTGACC G^GT[ACGT]ACC
5209 GGTGACC
BstPI 3769 GGTGACC G^GT[ACGT]ACC
5209 GGTGACC
EarI 5320 GAAGAG CTCTTC(1/4)
5332 GAAGAG
EcoO65I 3769 GGTGACC G^GT[ACGT]ACC
5209 GGTGACC
FbaI 2819 TGATCA T^GATCA
5414 TGATCA
Hin1I 399 GGCGCC G[GA]^CG[CT]C
970 GGCGCC
HindIII 5431 AAGCTT A^AGCTT
5488 AAGCTT
KasI 399 GGCGCC G^GCGCC
970 GGCGCC
KpnI 1247 GGTACC GGTAC^C
1286 GGTACC
Ksp632I 5320 GAAGAG CTCTTC(1/4)
5332 GAAGAG
MamI 3410 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5172 GATTGAGATC
NarI 399 GGCGCC GG^CGCC
970 GGCGCC
PshAI 1217 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3192 GACTCAGGTC
SfoI 399 GGCGCC GGC^GCC
970 GGCGCC
SmlI 1811 CTCAAG C^T[CT][GA]AG
5026 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KspI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeI NdeII
NgoMIV NlaIII NlaIV NotI NsiI
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI