################################## # NO CUT ENZYMES ( 32 enzymes) # ################################## AclI AscI AseI AsnI AvrII BlnI BsiWI BssHII Bst1107I BstBI BstZ17I DraI EcoRV HpaI MfeI MluI MunI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI ScaI SfuI SnaBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AatII 12286 GACGTC GACGT^C Acc65I 9470 GGTACC G^GTACC AflII 9154 CTTAAG C^TTAAG AocI 13603 CCTGAGG CC^T[ACGT]AGG Asp718I 9470 GGTACC G^GTACC BclI 5406 TGATCA T^GATCA BfrI 9154 CTTAAG C^TTAAG BglII 3959 AGATCT A^GATCT BsmI 11677 GCATTC GAATGC(1/-1) Bsu36I 13603 CCTGAGG CC^T[ACGT]AGG Eco81I 13603 CCTGAGG CC^T[ACGT]AGG EcoT22I 3222 ATGCAT ATGCA^T FbaI 5406 TGATCA T^GATCA FseI 1597 GGCCGGCC GGCCGG^CC HindIII 12193 AAGCTT A^AGCTT KpnI 9470 GGTACC GGTAC^C NdeI 513 CATATG CA^TATG NheI 10006 GCTAGC G^CTAGC NsiI 3222 ATGCAT ATGCA^T SalI 1084 GTCGAC G^TCGAC SgrAI 11821 CACCGGCG C[GA]^CCGG[CT]G SpeI 3997 ACTAGT A^CTAGT XbaI 6018 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 4130 TCCGGA T^CCGGA 5636 TCCGGA AgeI 2714 ACCGGT A^CCGGT 9104 ACCGGT AhdI 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4354 GACGCCAGGTC Alw44I 4276 GTGCAC G^TGCAC 5616 GTGCAC ApaLI 4276 GTGCAC G^TGCAC 5616 GTGCAC AspEI 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4354 GACGCCAGGTC BseAI 4130 TCCGGA T^CCGGA 5636 TCCGGA Bsp1407I 8438 TGTACA T^GTACA 11609 TGTACA BspEI 4130 TCCGGA T^CCGGA 5636 TCCGGA BsrGI 8438 TGTACA T^GTACA 11609 TGTACA CpoI 2690 CGGACCG CG^G[AT]CCG 4675 CGGACCG Eam1105I 1543 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4354 GACGCCAGGTC EcoNI 3598 CCTGGCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 9714 CCTGTTTGAGG EcoRI 5708 GAATTC G^AATTC 7560 GAATTC KspI 3966 CCGCGG CCGC^GG 9039 CCGCGG MroI 4130 TCCGGA T^CCGGA 5636 TCCGGA PinAI 2714 ACCGGT A^CCGGT 9104 ACCGGT RsrII 2690 CGGACCG CG^G[AT]CCG 4675 CGGACCG SacII 3966 CCGCGG CCGC^GG 9039 CCGCGG SbfI 3431 CCTGCAGG CCTGCA^GG 4572 CCTGCAGG SfiI 5218 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 5227 GGCCCCGTGGGCC SphI 779 GCATGC GCATG^C 12755 GCATGC Sse8387I 3431 CCTGCAGG CCTGCA^GG 4572 CCTGCAGG SspBI 8438 TGTACA T^GTACA 11609 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 179 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI