Restriction map for
pF1KE2461
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BfrI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstF5I
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FokI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1582 GTCTAC GT^[AC][GT]AC
AccII 899 CGCG CG^CG
Alw44I 1395 GTGCAC G^TGCAC
AlwNI 732 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 874 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1367 AGCGCT AGC^GCT
ApaLI 1395 GTGCAC G^TGCAC
AvaI 169 CCCGAG C^[CT]CG[GA]G
BbsI 81 GTCTTC GAAGAC(2/6)
BcgI 1640 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1289 TGATCA T^GATCA
BcnI 103 CCCGG CC^[GC]GG
BpmI 653 CTCCAG CTGGAG(16/14)
BpuAI 81 GTCTTC GAAGAC(2/6)
BsaAI 1675 TACGTG [CT]AC^GT[GA]
BsgI 9 CTGCAC GTGCAG(16/14)
BsoBI 169 CCCGAG C^[CT]CG[GA]G
BssSI 1740 CACGAG CACGAG(-5/-1)
BstEII 174 GGTGACC G^GT[ACGT]ACC
BstPI 174 GGTGACC G^GT[ACGT]ACC
BstUI 899 CGCG CG^CG
Bsu36I 874 CCTGAGG CC^T[ACGT]AGG
DraIII 514 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1367 AGCGCT AGC^GCT
Eco81I 874 CCTGAGG CC^T[ACGT]AGG
EcoO65I 174 GGTGACC G^GT[ACGT]ACC
FauI 897 CCCGC CCCGC(4/6)
FbaI 1289 TGATCA T^GATCA
HapII 104 CCGG C^CGG
HgaI 1580 GCGTC GACGC(5/10)
HpaII 104 CCGG C^CGG
MaeII 1676 ACGT A^CGT
MseI 554 TTAA T^TAA
MspI 104 CCGG C^CGG
MvnI 899 CGCG CG^CG
NciI 103 CCCGG CC^[GC]GG
PflMI 753 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 811 GGGACCT [GA]G^G[AT]CC[CT]
SacI 382 GAGCTC GAGCT^C
SapI 1536 GCTCTTC GCTCTTC(1/4)
SexAI 707 ACCAGGT A^CC[AT]GGT
SmlI 1390 CTTGAG C^T[CT][GA]AG
Tru9I 554 TTAA T^TAA
Tsp509I 591 AATT ^AATT
Van91I 753 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 281 CCAACATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Acc65I 296 GGTACC G^GTACC
1233 GGTACC
Asp718I 296 GGTACC G^GTACC
1233 GGTACC
BfaI 1012 CTAG C^TAG
1355 CTAG
BglI 20 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1091 GCCTCTATGGC
BseRI 1447 CTCCTC GAGGAG(10/8)
250 GAGGAG
BstYI 509 AGATCC [GA]^GATC[CT]
567 AGATCC
DraII 811 GGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
EaeI 1574 CGGCCA [CT]^GGCC[GA]
1607 CGGCCA
EcoNI 222 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1479 CCTGGGTAAGG
EcoO109I 811 GGGACCT [GA]G^G[ACGT]CC[CT]
1142 AGGCCCT
HaeII 1367 AGCGCT [GA]GCGC^[CT]
1745 GGCGCT
HinfI 79 GAGTC G^A[ACGT]TC
322 GACTC
KpnI 296 GGTACC GGTAC^C
1233 GGTACC
MaeI 1012 CTAG C^TAG
1355 CTAG
MflI 509 AGATCC [GA]^GATC[CT]
567 AGATCC
MslI 903 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1017 CACCCCTGTG
PleI 322 GACTC GAGTC(4/5)
79 GAGTC
PvuII 301 CAGCTG CAG^CTG
1306 CAGCTG
SfaNI 842 GATGC GCATC(5/9)
1386 GATGC
XhoII 509 AGATCC [GA]^GATC[CT]
567 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvI BlpI
BmyI Bpu1102I BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI BstXI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI HaeIII HhaI HinP1I HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI StuI StyI
TseI Tsp45I TspRI