Restriction map for
pF1KB9941
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MamI
MluI MseI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 364 TCCGGA T^CCGGA
AflIII 1319 ACGTGT A^C[GA][CT]GT
AocI 1146 CCTGAGG CC^T[ACGT]AGG
Asp700I 900 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1348 GAGCTC G[AT]GC[AT]^C
AvaI 1573 CCCGGG C^[CT]CG[GA]G
BamHI 1334 GGATCC G^GATCC
BbrPI 840 CACGTG CAC^GTG
BbsI 870 GAAGAC GAAGAC(2/6)
BbvCI 1099 GCTGAGG CCTCAGC(-5/-2)
BpuAI 870 GAAGAC GAAGAC(2/6)
BsaWI 364 TCCGGA [AT]^CCGG[AT]
BseAI 364 TCCGGA T^CCGGA
BsiHKAI 1348 GAGCTC G[AT]GC[AT]^C
BsmBI 1213 CGTCTC CGTCTC(1/5)
BsmI 380 GCATTC GAATGC(1/-1)
BsoBI 1573 CCCGGG C^[CT]CG[GA]G
BspEI 364 TCCGGA T^CCGGA
BsrBI 1174 GAGCGG CCGCTC(-3/-3)
BsrDI 246 CATTGC GCAATG(2/0)
BstAPI 809 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 234 GGTGACC G^GT[ACGT]ACC
BstPI 234 GGTGACC G^GT[ACGT]ACC
Bsu36I 1146 CCTGAGG CC^T[ACGT]AGG
DraII 496 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 424 GAAGAG CTCTTC(1/4)
Eco81I 1146 CCTGAGG CC^T[ACGT]AGG
EcoO109I 496 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 234 GGTGACC G^GT[ACGT]ACC
HaeII 797 AGCGCC [GA]GCGC^[CT]
HincII 406 GTTGAC GT[CT]^[GA]AC
HindII 406 GTTGAC GT[CT]^[GA]AC
Ksp632I 424 GAAGAG CTCTTC(1/4)
MfeI 1072 CAATTG C^AATTG
MroI 364 TCCGGA T^CCGGA
MunI 1072 CAATTG C^AATTG
NaeI 800 GCCGGC GCC^GGC
NcoI 1469 CCATGG C^CATGG
NgoMIV 800 GCCGGC G^CCGGC
NspI 1427 ACATGC [GA]CATG^[CT]
PleI 409 GACTC GAGTC(4/5)
PmaCI 840 CACGTG CAC^GTG
PmlI 840 CACGTG CAC^GTG
PstI 1522 CTGCAG CTGCA^G
SacI 1348 GAGCTC GAGCT^C
SmaI 1573 CCCGGG CCC^GGG
SmlI 1036 CTCAAG C^T[CT][GA]AG
TspRI 1344 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 386 CCAGCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1573 CCCGGG C^CCGGG
XmaI 1573 CCCGGG C^CCGGG
XmnI 900 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 760 GACGCC G[GA]^CG[CT]C
1484 GGCGTC
AhdI 1318 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCTGGCGTC
AspEI 1318 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCTGGCGTC
AvaII 1325 GGTCC G^G[AT]CC
1491 GGACC
BalI 35 TGGCCA TGG^CCA
752 TGGCCA
BanII 982 GAGCCC G[GA]GC[CT]^C
1348 GAGCTC
BglI 800 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1436 GCCCTCCAGGC
BsaAI 394 TACGTG [CT]AC^GT[GA]
840 CACGTG
BsaHI 760 GACGCC G[GA]^CG[CT]C
1484 GGCGTC
BsaI 520 GAGACC GGTCTC(1/5)
353 GGTCTC
BspHI 728 TCATGA T^CATGA
926 TCATGA
BstXI 785 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1157 CCAACATCCTGG
BstYI 596 AGATCC [GA]^GATC[CT]
1334 GGATCC
Eam1105I 1318 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCTGGCGTC
Hin1I 760 GACGCC G[GA]^CG[CT]C
1484 GGCGTC
MboII 424 GAAGA GAAGA(8/7)
870 GAAGA
MflI 596 AGATCC [GA]^GATC[CT]
1334 GGATCC
MluNI 35 TGGCCA TGG^CCA
752 TGGCCA
MscI 35 TGGCCA TGG^CCA
752 TGGCCA
PflMI 1071 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1262 CCATCAAGTGG
PvuII 469 CAGCTG CAG^CTG
958 CAGCTG
RcaI 728 TCATGA T^CATGA
926 TCATGA
SfcI 156 CTACAG C^T[GA][CT]AG
1522 CTGCAG
StuI 902 AGGCCT AGG^CCT
1504 AGGCCT
TaqI 270 TCGA T^CGA
1514 TCGA
TfiI 1301 GATTC G^A[AT]TC
1367 GAATC
Tsp509I 23 AATT ^AATT
1073 AATT
TthHB8I 270 TCGA T^CGA
1514 TCGA
Van91I 1071 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1262 CCATCAAGTGG
XhoII 596 AGATCC [GA]^GATC[CT]
1334 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I