################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 190 GTCTAC GT^[AC][GT]AC AciI 777 GCGG CCGC(-3/-1) Alw44I 788 GTGCAC G^TGCAC AocI 304 CCTGAGG CC^T[ACGT]AGG ApaLI 788 GTGCAC G^TGCAC Asp700I 159 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 477 CCCGGG C^[CT]CG[GA]G AvaII 77 GGTCC G^G[AT]CC BamHI 53 GGATCC G^GATCC BanI 718 GGCACC G^G[CT][GA]CC BbsI 424 GTCTTC GAAGAC(2/6) BbvI 780 GCTGC GCAGC(8/12) BlpI 255 GCTGAGC GC^T[ACGT]AGC Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC BpuAI 424 GTCTTC GAAGAC(2/6) BsaAI 112 TACGTG [CT]AC^GT[GA] BsaI 633 GGTCTC GGTCTC(1/5) BsgI 640 CTGCAC GTGCAG(16/14) BsmAI 634 GTCTC GTCTC(1/5) BsmFI 78 GTCCC GGGAC(10/14) BsoBI 477 CCCGGG C^[CT]CG[GA]G BspHI 182 TCATGA T^CATGA BspLU11I 420 ACATGT A^CATGT BspMI 405 GCAGGT ACCTGC(4/8) BstEII 408 GGTGACC G^GT[ACGT]ACC BstPI 408 GGTGACC G^GT[ACGT]ACC BstXI 168 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 304 CCTGAGG CC^T[ACGT]AGG CelII 255 GCTGAGC GC^T[ACGT]AGC CfoI 273 GCGC GCG^C DraII 76 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 433 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 174 CCGTGG C^C[GA][CT]GG Eco81I 304 CCTGAGG CC^T[ACGT]AGG EcoO109I 76 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 408 GGTGACC G^GT[ACGT]ACC HaeII 272 GGCGCT [GA]GCGC^[CT] HhaI 273 GCGC GCG^C HinP1I 273 GCGC G^CGC MaeII 113 ACGT A^CGT MslI 705 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 743 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 182 TCATGA T^CATGA SacI 806 GAGCTC GAGCT^C SapI 336 GCTCTTC GCTCTTC(1/4) ScaI 653 AGTACT AGT^ACT SfaNI 143 GCATC GCATC(5/9) SmaI 477 CCCGGG CCC^GGG TseI 780 GCTGC G^C[AT]GC Van91I 743 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 477 CCCGGG C^CCGGG XmaI 477 CCCGGG C^CCGGG XmnI 159 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 113 ACGTGT A^C[GA][CT]GT 420 ACATGT BanII 489 GGGCTC G[GA]GC[CT]^C 806 GAGCTC BpmI 809 CTCCAG CTGGAG(16/14) 447 CTGGAG BstF5I 317 GGATG GGATG(2/0) 710 GGATG BstYI 45 AGATCC [GA]^GATC[CT] 53 GGATCC EcoT14I 767 CCAAGG C^C[AT][AT]GG 821 CCAAGG Fnu4HI 777 GCGGC GC^[ACGT]GC 780 GCTGC FokI 317 GGATG GGATG(9/13) 710 GGATG HapII 51 CCGG C^CGG 478 CCGG HpaII 51 CCGG C^CGG 478 CCGG ItaI 777 GCGGC GC^[ACGT]GC 780 GCTGC MflI 45 AGATCC [GA]^GATC[CT] 53 GGATCC MspA1I 613 CAGCTG C[AC]G^C[GT]G 669 CAGCTG MspI 51 CCGG C^CGG 478 CCGG MwoI 162 GCCCTTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 251 GCTGGCTGAGC NspI 420 ACATGT [GA]CATG^[CT] 522 GCATGT PvuII 613 CAGCTG CAG^CTG 669 CAGCTG StyI 767 CCAAGG C^C[AT][AT]GG 821 CCAAGG Tsp45I 278 GTGAC ^GT[GC]AC 409 GTGAC XhoII 45 AGATCC [GA]^GATC[CT] 53 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI AlwI AspHI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII HaeIII HphI Ksp632I MaeIII MboI MboII MnlI MvaI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TspRI