################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DraIII Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MfeI MflI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 1937 ACATGT A^C[GA][CT]GT Alw44I 197 GTGCAC G^TGCAC ApaLI 197 GTGCAC G^TGCAC BanI 3 GGTGCC G^G[CT][GA]CC BbvCI 72 CCTCAGC CCTCAGC(-5/-2) BciVI 627 GTATCC GTATCC(6/5) BfaI 979 CTAG C^TAG BlpI 1380 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1380 GCTGAGC GC^T[ACGT]AGC BsaWI 479 TCCGGT [AT]^CCGG[AT] BsgI 1234 GTGCAG GTGCAG(16/14) BsiEI 1488 CGGCCG CG[GA][CT]^CG BspHI 1848 TCATGA T^CATGA BspLU11I 1937 ACATGT A^CATGT BspMI 101 ACCTGC ACCTGC(4/8) BsrFI 79 GCCGGC [GA]^CCGG[CT] BssHII 21 GCGCGC G^CGCGC BssSI 1764 CACGAG CACGAG(-5/-1) CelII 1380 GCTGAGC GC^T[ACGT]AGC Cfr10I 79 GCCGGC [GA]^CCGG[CT] DraI 1162 TTTAAA TTT^AAA DrdI 1219 GACTCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1488 CGGCCG C^GGCCG EclXI 1488 CGGCCG C^GGCCG Eco52I 1488 CGGCCG C^GGCCG EcoNI 1544 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1360 GAATTC G^AATTC HaeII 76 AGCGCC [GA]GCGC^[CT] KspI 41 CCGCGG CCGC^GG MaeI 979 CTAG C^TAG MslI 1030 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 79 GCCGGC GCC^GGC NcoI 1614 CCATGG C^CATGG NgoMIV 79 GCCGGC G^CCGGC NspI 1937 ACATGT [GA]CATG^[CT] PaeR7I 368 CTCGAG C^TCGAG PflMI 1911 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 103 CTGCAG CTGCA^G RcaI 1848 TCATGA T^CATGA SacII 41 CCGCGG CCGC^GG SexAI 614 ACCTGGT A^CC[AT]GGT SmlI 368 CTCGAG C^T[CT][GA]AG Van91I 1911 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 719 CCAGACACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 368 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 22 CGCG CG^CG 42 CGCG AcsI 747 AAATTT [GA]^AATT[CT] 1360 GAATTC AlwI 771 GGATC GGATC(4/5) 1700 GGATC AlwNI 97 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 910 CAGAAGCTG AocI 735 CCTAAGG CC^T[ACGT]AGG 942 CCTGAGG ApaI 36 GGGCCC GGGCC^C 88 GGGCCC ApoI 747 AAATTT [GA]^AATT[CT] 1360 GAATTC AspI 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1330 GACTGCGTC BglI 1399 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1557 GCCCTCCAGGC BpmI 1560 CTCCAG CTGGAG(16/14) 511 CTGGAG BsaI 1498 GAGACC GGTCTC(1/5) 1815 GGTCTC BseRI 934 GAGGAG GAGGAG(10/8) 1349 GAGGAG BsmI 1408 GCATTC GAATGC(1/-1) 1477 GCATTC BstUI 22 CGCG CG^CG 42 CGCG Bsu36I 735 CCTAAGG CC^T[ACGT]AGG 942 CCTGAGG DpnII 772 GATC ^GATC 1701 GATC DsaI 41 CCGCGG C^C[GA][CT]GG 1614 CCATGG EarI 700 CTCTTC CTCTTC(1/4) 1875 CTCTTC Eco81I 735 CCTAAGG CC^T[ACGT]AGG 942 CCTGAGG HgaI 1390 GACGC GACGC(5/10) 1334 GCGTC Ksp632I 700 CTCTTC CTCTTC(1/4) 1875 CTCTTC MboI 772 GATC ^GATC 1701 GATC MvnI 22 CGCG CG^CG 42 CGCG NdeII 772 GATC ^GATC 1701 GATC PflFI 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1330 GACTGCGTC PpuMI 339 GGGACCC [GA]G^G[AT]CC[CT] 998 AGGACCT SacI 901 GAGCTC GAGCT^C 1714 GAGCTC Sau3AI 772 GATC ^GATC 1701 GATC SfcI 103 CTGCAG C^T[GA][CT]AG 1464 CTGTAG StuI 1541 AGGCCT AGG^CCT 1925 AGGCCT TaqI 369 TCGA T^CGA 1250 TCGA Tth111I 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1330 GACTGCGTC TthHB8I 369 TCGA T^CGA 1250 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PvuII RsaI Sau96I ScrFI SfaNI SmaI StyI Tru9I TseI Tsp45I Tsp509I TspRI XmaCI XmaI