Restriction map for
pF1KE2566
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 112 GTATAC GT^[AC][GT]AC
AhdI 316 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1609 GTGCAC G^TGCAC
ApaLI 1609 GTGCAC G^TGCAC
AspEI 316 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 450 GGATCC G^GATCC
BanI 1600 GGCACC G^G[CT][GA]CC
BbsI 260 GAAGAC GAAGAC(2/6)
BbvCI 358 GCTGAGG CCTCAGC(-5/-2)
BclI 302 TGATCA T^GATCA
BcnI 757 CCGGG CC^[GC]GG
BpuAI 260 GAAGAC GAAGAC(2/6)
BseRI 1523 GAGGAG GAGGAG(10/8)
BsgI 1654 CTGCAC GTGCAG(16/14)
BsmBI 88 GAGACG CGTCTC(1/5)
BspHI 185 TCATGA T^CATGA
BssSI 1264 CTCGTG CACGAG(-5/-1)
Bst1107I 112 GTATAC GTA^TAC
BstYI 450 GGATCC [GA]^GATC[CT]
BstZ17I 112 GTATAC GTA^TAC
DraII 1470 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 701 CCATGG C^C[GA][CT]GG
Eam1105I 316 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1462 CCTTATTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1470 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1501 GATATC GAT^ATC
EcoT14I 701 CCATGG C^C[AT][AT]GG
FbaI 302 TGATCA T^GATCA
HapII 757 CCGG C^CGG
HpaII 757 CCGG C^CGG
MflI 450 GGATCC [GA]^GATC[CT]
MspA1I 51 CAGCGG C[AC]G^C[GT]G
MspI 757 CCGG C^CGG
NciI 757 CCGGG CC^[GC]GG
NcoI 701 CCATGG C^CATGG
NspI 990 GCATGT [GA]CATG^[CT]
PleI 1298 GAGTC GAGTC(4/5)
RcaI 185 TCATGA T^CATGA
ScaI 69 AGTACT AGT^ACT
SspI 418 AATATT AAT^ATT
StyI 701 CCATGG C^C[AT][AT]GG
XbaI 466 TCTAGA T^CTAGA
XcmI 1325 CCAAAGATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 450 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 774 CTTAAG C^TTAAG
1444 CTTAAG
AocI 1227 CCTCAGG CC^T[ACGT]AGG
1495 CCTCAGG
Asp700I 874 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1620 GAAAAGTTTC
BanII 253 GAGCTC G[GA]GC[CT]^C
1674 GAGCTC
BfrI 774 CTTAAG C^TTAAG
1444 CTTAAG
BsiYI 1462 CCTTATTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1662 CCGAAGCAAGG
BslI 1462 CCTTATTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1662 CCGAAGCAAGG
BsmFI 976 GGGAC GGGAC(10/14)
515 GTCCC
Bsu36I 1227 CCTCAGG CC^T[ACGT]AGG
1495 CCTCAGG
EarI 1315 CTCTTC CTCTTC(1/4)
1537 GAAGAG
Eco81I 1227 CCTCAGG CC^T[ACGT]AGG
1495 CCTCAGG
EcoRI 607 GAATTC G^AATTC
808 GAATTC
FauI 1154 CCCGC CCCGC(4/6)
53 GCGGG
Ksp632I 1315 CTCTTC CTCTTC(1/4)
1537 GAAGAG
MaeII 5 ACGT A^CGT
317 ACGT
MwoI 1196 GCAGCAATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1667 GCAAGGAGAGC
PstI 1120 CTGCAG CTGCA^G
1271 CTGCAG
SacI 253 GAGCTC GAGCT^C
1674 GAGCTC
XmnI 874 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1620 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsmAI
Bsp1286I BsrDI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I