Restriction map for
pF1KE3359
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
AspEI AvrII BclI BfaI BfrI
BglII BlnI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BstBI
ClaI DraI DrdI Eam1105I EcoT22I
FbaI FseI HpaI MaeI MluI
MroI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RcaI SalI
SfiI SfuI SmaI SnaBI SpeI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 1337 GGTACC G^GTACC
AccI 2164 GTATAC GT^[AC][GT]AC
AflIII 1701 ACGTGT A^C[GA][CT]GT
AocI 2199 CCTGAGG CC^T[ACGT]AGG
AscI 201 GGCGCGCC GG^CGCGCC
Asp700I 2123 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1337 GGTACC G^GTACC
AspI 1924 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 128 TGGCCA TGG^CCA
BamHI 1239 GGATCC G^GATCC
BbrPI 2218 CACGTG CAC^GTG
BcgI 1245 CGAGAATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2572 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2572 GCTGAGC GC^T[ACGT]AGC
BsaAI 2218 CACGTG [CT]AC^GT[GA]
BsaBI 1234 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3055 GAGACC GGTCTC(1/5)
BsaWI 1904 TCCGGT [AT]^CCGG[AT]
BsmBI 2593 GAGACG CGTCTC(1/5)
BsmI 1454 GCATTC GAATGC(1/-1)
Bst1107I 2164 GTATAC GTA^TAC
BstAPI 831 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2691 GGTGACC G^GT[ACGT]ACC
BstPI 2691 GGTGACC G^GT[ACGT]ACC
BstZ17I 2164 GTATAC GTA^TAC
Bsu36I 2199 CCTGAGG CC^T[ACGT]AGG
CelII 2572 GCTGAGC GC^T[ACGT]AGC
CpoI 230 CGGTCCG CG^G[AT]CCG
DraIII 2563 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 565 CGGCCG C^GGCCG
EarI 577 GAAGAG CTCTTC(1/4)
EclXI 565 CGGCCG C^GGCCG
Eco52I 565 CGGCCG C^GGCCG
Eco81I 2199 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2691 GGTGACC G^GT[ACGT]ACC
EcoRI 970 GAATTC G^AATTC
EcoRV 820 GATATC GAT^ATC
HgaI 269 GCGTC GACGC(5/10)
HincII 2995 GTCAAC GT[CT]^[GA]AC
HindII 2995 GTCAAC GT[CT]^[GA]AC
HindIII 1161 AAGCTT A^AGCTT
KpnI 1337 GGTACC GGTAC^C
Ksp632I 577 GAAGAG CTCTTC(1/4)
KspI 299 CCGCGG CCGC^GG
MamI 1234 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2765 CAATTG C^AATTG
MluNI 128 TGGCCA TGG^CCA
MscI 128 TGGCCA TGG^CCA
MunI 2765 CAATTG C^AATTG
NdeI 1756 CATATG CA^TATG
PflFI 1924 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1290 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2218 CACGTG CAC^GTG
PmlI 2218 CACGTG CAC^GTG
PvuI 1206 CGATCG CGAT^CG
RsrII 230 CGGTCCG CG^G[AT]CCG
SacI 2113 GAGCTC GAGCT^C
SacII 299 CCGCGG CCGC^GG
SapI 577 GAAGAGC GCTCTTC(1/4)
SbfI 1857 CCTGCAGG CCTGCA^GG
SexAI 1786 ACCAGGT A^CC[AT]GGT
SgrAI 414 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1857 CCTGCAGG CCTGCA^GG
SspI 634 AATATT AAT^ATT
StuI 911 AGGCCT AGG^CCT
TfiI 2879 GAATC G^A[AT]TC
Tth111I 1924 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1290 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3059 CCAAGGCTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2123 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 970 GAATTC [GA]^AATT[CT]
2407 AAATTT
Aor51HI 543 AGCGCT AGC^GCT
2658 AGCGCT
ApoI 970 GAATTC [GA]^AATT[CT]
2407 AAATTT
AviII 2402 TGCGCA TGC^GCA
2912 TGCGCA
BbeI 14 GGCGCC GGCGC^C
418 GGCGCC
BciVI 1180 GGATAC GTATCC(6/5)
2988 GGATAC
Bsp1407I 2711 TGTACA T^GTACA
2750 TGTACA
BsrGI 2711 TGTACA T^GTACA
2750 TGTACA
BssHII 157 GCGCGC G^CGCGC
202 GCGCGC
BssSI 595 CTCGTG CACGAG(-5/-1)
1687 CTCGTG
Eco47III 543 AGCGCT AGC^GCT
2658 AGCGCT
EcoNI 186 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
594 CCTCGTGGAGG
FspI 2402 TGCGCA TGC^GCA
2912 TGCGCA
KasI 14 GGCGCC G^GCGCC
418 GGCGCC
MseI 985 TTAA T^TAA
2411 TTAA
NaeI 415 GCCGGC GCC^GGC
1586 GCCGGC
NarI 14 GGCGCC GG^CGCC
418 GGCGCC
NcoI 1019 CCATGG C^CATGG
1971 CCATGG
NgoMIV 415 GCCGGC G^CCGGC
1586 GCCGGC
NspI 1387 GCATGC [GA]CATG^[CT]
1937 GCATGC
ScaI 962 AGTACT AGT^ACT
1838 AGTACT
SfoI 14 GGCGCC GGC^GCC
418 GGCGCC
SphI 1387 GCATGC GCATG^C
1937 GCATGC
SspBI 2711 TGTACA T^GTACA
2750 TGTACA
Tru9I 985 TTAA T^TAA
2411 TTAA
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII