Restriction map for
pF1KE5338
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# NO CUT ENZYMES ( 150 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KasI Ksp632I KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TfiI
Tsp45I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 439 GACGTC GACGT^C
Acc65I 355 GGTACC G^GTACC
AccII 510 CGCG CG^CG
AcyI 439 GACGTC G[GA]^CG[CT]C
AlwNI 314 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 355 GGTACC G^GTACC
AvaI 623 CCCGGG C^[CT]CG[GA]G
BalI 545 TGGCCA TGG^CCA
BciVI 627 GGATAC GTATCC(6/5)
BfaI 689 CTAG C^TAG
BsaHI 439 GACGTC G[GA]^CG[CT]C
BseRI 40 CTCCTC GAGGAG(10/8)
BsiEI 699 CGATCG CG[GA][CT]^CG
BsmI 180 GAATGC GAATGC(1/-1)
BsoBI 623 CCCGGG C^[CT]CG[GA]G
BspMI 727 GCAGGT ACCTGC(4/8)
BsrDI 561 CATTGC GCAATG(2/0)
BstF5I 637 CATCC GGATG(2/0)
BstUI 510 CGCG CG^CG
BstXI 663 CCACACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 288 GGGCC G^G[ACGT]CC
DraIII 370 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 669 CCATGG C^C[GA][CT]GG
EaeI 545 TGGCCA [CT]^GGCC[GA]
FauI 76 CCCGC CCCGC(4/6)
FokI 637 CATCC GGATG(9/13)
HaeII 674 GGCGCT [GA]GCGC^[CT]
Hin1I 439 GACGTC G[GA]^CG[CT]C
HinfI 336 GACTC G^A[ACGT]TC
KpnI 355 GGTACC GGTAC^C
MaeI 689 CTAG C^TAG
MaeIII 88 GTTAC ^GT[ACGT]AC
MfeI 331 CAATTG C^AATTG
MluNI 545 TGGCCA TGG^CCA
MscI 545 TGGCCA TGG^CCA
MslI 664 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 604 CAGCTG C[AC]G^C[GT]G
MunI 331 CAATTG C^AATTG
MvnI 510 CGCG CG^CG
NcoI 669 CCATGG C^CATGG
PflMI 369 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 336 GACTC GAGTC(4/5)
PvuI 699 CGATCG CGAT^CG
PvuII 604 CAGCTG CAG^CTG
Sau96I 288 GGGCC G^G[ACGT]CC
SfaNI 506 GCATC GCATC(5/9)
SmaI 623 CCCGGG CCC^GGG
SspI 298 AATATT AAT^ATT
TaqI 417 TCGA T^CGA
TthHB8I 417 TCGA T^CGA
Van91I 369 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 477 CCAGTCTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 623 CCCGGG C^CCGGG
XmaI 623 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 77 CCGC CCGC(-3/-1)
585 CCGC
AlwI 205 GATCC GGATC(4/5)
316 GGATC
BsrI 15 CCAGT ACTGG(1/-1)
477 CCAGT
BstYI 204 AGATCC [GA]^GATC[CT]
316 GGATCT
Cac8I 106 GCAAGC GC[ACGT]^[ACGT]GC
543 GCTGGC
DdeI 173 CTGAG C^T[ACGT]AG
435 CTCAG
EcoT14I 566 CCTTGG C^C[AT][AT]GG
669 CCATGG
HapII 7 CCGG C^CGG
624 CCGG
HpaII 7 CCGG C^CGG
624 CCGG
MaeII 374 ACGT A^CGT
440 ACGT
MflI 204 AGATCC [GA]^GATC[CT]
316 GGATCT
MseI 188 TTAA T^TAA
302 TTAA
MspI 7 CCGG C^CGG
624 CCGG
NlaIII 103 CATG CATG^
670 CATG
PstI 232 CTGCAG CTGCA^G
725 CTGCAG
SfcI 232 CTGCAG C^T[GA][CT]AG
725 CTGCAG
StyI 566 CCTTGG C^C[AT][AT]GG
669 CCATGG
Tru9I 188 TTAA T^TAA
302 TTAA
Tsp509I 190 AATT ^AATT
332 AATT
XhoII 204 AGATCC [GA]^GATC[CT]
316 GGATCT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI CfoI DpnII
EcoRII Fnu4HI HaeIII HhaI HinP1I
ItaI MboI MboII MnlI MvaI
MwoI NciI NdeII NlaIV RsaI
Sau3AI ScrFI TseI TspRI