Restriction map for
pF1KB7170
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FspI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
ScaI SexAI SfuI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AspI 190 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 653 TGGCCA TGG^CCA
BsmAI 472 GAGAC GTCTC(1/5)
BsmBI 472 GAGACG CGTCTC(1/5)
BsrI 690 CCAGT ACTGG(1/-1)
BssSI 1234 CACGAG CACGAG(-5/-1)
BstAPI 1065 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 609 CATCC GGATG(2/0)
BstXI 59 CCACCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 44 AGATCC [GA]^GATC[CT]
FokI 609 CATCC GGATG(9/13)
FseI 1219 GGCCGGCC GGCCGG^CC
MflI 44 AGATCC [GA]^GATC[CT]
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
MslI 60 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 190 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 568 GGGACCC [GA]G^G[AT]CC[CT]
SbfI 1062 CCTGCAGG CCTGCA^GG
SfiI 898 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 492 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1062 CCTGCAGG CCTGCA^GG
Tth111I 190 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 437 CCATGCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 44 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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Alw44I 295 GTGCAC G^TGCAC
531 GTGCAC
AlwI 45 GATCC GGATC(4/5)
1313 GGATC
ApaI 576 GGGCCC GGGCC^C
1147 GGGCCC
ApaLI 295 GTGCAC G^TGCAC
531 GTGCAC
AspHI 295 GTGCAC G[AT]GC[AT]^C
531 GTGCAC
AvaII 569 GGACC G^G[AT]CC
1095 GGACC
BbrPI 226 CACGTG CAC^GTG
754 CACGTG
BpmI 15 CTGGAG CTGGAG(16/14)
37 CTGGAG
BsiHKAI 295 GTGCAC G[AT]GC[AT]^C
531 GTGCAC
BspMI 380 ACCTGC ACCTGC(4/8)
695 ACCTGC
BsrBI 325 GAGCGG CCGCTC(-3/-3)
1237 GAGCGG
DpnII 45 GATC ^GATC
1314 GATC
DraIII 754 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1305 CACCGTGTG
DsaI 903 CCGCGG C^C[GA][CT]GG
937 CCGCGG
EcoNI 420 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
447 CCTGGGCGAGG
KspI 903 CCGCGG CCGC^GG
937 CCGCGG
MboI 45 GATC ^GATC
1314 GATC
NdeII 45 GATC ^GATC
1314 GATC
NotI 896 GCGGCCGC GC^GGCCGC
967 GCGGCCGC
PmaCI 226 CACGTG CAC^GTG
754 CACGTG
PmlI 226 CACGTG CAC^GTG
754 CACGTG
PstI 732 CTGCAG CTGCA^G
1063 CTGCAG
PvuII 646 CAGCTG CAG^CTG
822 CAGCTG
SacII 903 CCGCGG CCGC^GG
937 CCGCGG
Sau3AI 45 GATC ^GATC
1314 GATC
TaqI 668 TCGA T^CGA
710 TCGA
TthHB8I 668 TCGA T^CGA
710 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpuAI BsaAI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV PflMI
RsaI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TseI TspRI Van91I
XmaCI XmaI