Restriction map for
pF1KE2347
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiWI BsmBI
BspDI BspHI BspLU11I BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eco47III EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI MamI MfeI MluI
MunI NcoI NdeI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 697 GTCTAC GT^[AC][GT]AC
AhdI 1713 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 135 CCTGAGG CC^T[ACGT]AGG
Asp700I 1455 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1713 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 645 CCTAGG C^CTAGG
BcgI 1122 CGAGAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 645 CCTAGG C^CTAGG
BsiEI 452 CGGCCG CG[GA][CT]^CG
BsmAI 928 GTCTC GTCTC(1/5)
Bsp1407I 1241 TGTACA T^GTACA
BspMI 299 GCAGGT ACCTGC(4/8)
BsrBI 207 GAGCGG CCGCTC(-3/-3)
BsrGI 1241 TGTACA T^GTACA
Bsu36I 135 CCTGAGG CC^T[ACGT]AGG
EagI 452 CGGCCG C^GGCCG
Eam1105I 1713 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 452 CGGCCG C^GGCCG
Eco52I 452 CGGCCG C^GGCCG
Eco81I 135 CCTGAGG CC^T[ACGT]AGG
EcoRV 1001 GATATC GAT^ATC
HgaI 245 GCGTC GACGC(5/10)
HindIII 1458 AAGCTT A^AGCTT
KspI 92 CCGCGG CCGC^GG
NheI 1056 GCTAGC G^CTAGC
PaeR7I 34 CTCGAG C^TCGAG
PflMI 1809 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 112 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 297 CTGCAG CTGCA^G
SacII 92 CCGCGG CCGC^GG
SapI 2146 GAAGAGC GCTCTTC(1/4)
SmlI 34 CTCGAG C^T[CT][GA]AG
SspBI 1241 TGTACA T^GTACA
StuI 392 AGGCCT AGG^CCT
Van91I 1809 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2025 TCTAGA T^CTAGA
XcmI 602 CCAACCCCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 34 CTCGAG C^TCGAG
XmnI 1455 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 1213 TCCGGA T^CCGGA
1847 TCCGGA
AcsI 1851 GAATTT [GA]^AATT[CT]
1958 AAATTC
AlwI 1700 GATCC GGATC(4/5)
1558 GGATC
ApaI 163 GGGCCC GGGCC^C
890 GGGCCC
ApoI 1851 GAATTT [GA]^AATT[CT]
1958 AAATTC
BsaWI 1213 TCCGGA [AT]^CCGG[AT]
1847 TCCGGA
BseAI 1213 TCCGGA T^CCGGA
1847 TCCGGA
BsgI 836 CTGCAC GTGCAG(16/14)
1379 GTGCAG
BsmI 2088 GAATGC GAATGC(1/-1)
1174 GCATTC
BspEI 1213 TCCGGA T^CCGGA
1847 TCCGGA
BsrDI 981 CATTGC GCAATG(2/0)
1761 CATTGC
BssHII 193 GCGCGC G^CGCGC
342 GCGCGC
BstAPI 367 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1978 GCAACCAGTGC
BstYI 1558 GGATCT [GA]^GATC[CT]
1699 AGATCC
DsaI 92 CCGCGG C^C[GA][CT]GG
1280 CCGTGG
EarI 1293 CTCTTC CTCTTC(1/4)
2146 GAAGAG
HphI 1285 GGTGA GGTGA(8/7)
931 TCACC
Ksp632I 1293 CTCTTC CTCTTC(1/4)
2146 GAAGAG
MaeII 1068 ACGT A^CGT
1487 ACGT
MflI 1558 GGATCT [GA]^GATC[CT]
1699 AGATCC
MroI 1213 TCCGGA T^CCGGA
1847 TCCGGA
MslI 909 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CATGACCGTG
NaeI 141 GCCGGC GCC^GGC
408 GCCGGC
NgoMIV 141 GCCGGC G^CCGGC
408 GCCGGC
PshAI 1017 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1669 GACATCCGTC
PvuII 294 CAGCTG CAG^CTG
515 CAGCTG
SacI 1344 GAGCTC GAGCT^C
1399 GAGCTC
SfaNI 1993 GATGC GCATC(5/9)
908 GCATC
SfcI 297 CTGCAG C^T[GA][CT]AG
618 CTACAG
TfiI 1033 GATTC G^A[AT]TC
1950 GAATC
XhoII 1558 GGATCT [GA]^GATC[CT]
1699 AGATCC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfoI SmaI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI