Restriction map for
pF1KE2640
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI AlwI AocI Aor51HI
ApaI ApoI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BcgI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI Ksp632I MaeI MaeIII
MamI MfeI MflI MluI MroI
MseI MunI NdeI NheI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 410 GGTACC G^GTACC
Alw44I 39 GTGCAC G^TGCAC
ApaLI 39 GTGCAC G^TGCAC
AscI 449 GGCGCGCC GG^CGCGCC
Asp718I 410 GGTACC G^GTACC
AvaI 97 CCCGAG C^[CT]CG[GA]G
AviII 17 TGCGCA TGC^GCA
BalI 467 TGGCCA TGG^CCA
BbrPI 1011 CACGTG CAC^GTG
BbsI 73 GTCTTC GAAGAC(2/6)
BbvCI 159 CCTCAGC CCTCAGC(-5/-2)
BclI 980 TGATCA T^GATCA
BcnI 1039 CCGGG CC^[GC]GG
BglI 164 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 73 GTCTTC GAAGAC(2/6)
BseRI 178 GAGGAG GAGGAG(10/8)
BsiEI 1050 CGGCCG CG[GA][CT]^CG
BsmFI 992 GGGAC GGGAC(10/14)
BsoBI 97 CCCGAG C^[CT]CG[GA]G
DdeI 160 CTCAG C^T[ACGT]AG
DraIII 507 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1050 CGGCCG C^GGCCG
EclXI 1050 CGGCCG C^GGCCG
Eco52I 1050 CGGCCG C^GGCCG
FbaI 980 TGATCA T^GATCA
FspI 17 TGCGCA TGC^GCA
HinfI 88 GAGTC G^A[ACGT]TC
KpnI 410 GGTACC GGTAC^C
MluNI 467 TGGCCA TGG^CCA
MscI 467 TGGCCA TGG^CCA
NciI 1039 CCGGG CC^[GC]GG
NcoI 464 CCATGG C^CATGG
NotI 1049 GCGGCCGC GC^GGCCGC
NruI 1072 TCGCGA TCG^CGA
PflMI 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 88 GAGTC GAGTC(4/5)
PmaCI 1011 CACGTG CAC^GTG
PmlI 1011 CACGTG CAC^GTG
PpuMI 992 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1173 GAGCTC GAGCT^C
ScaI 965 AGTACT AGT^ACT
SmlI 205 CTCAAG C^T[CT][GA]AG
SphI 443 GCATGC GCATG^C
StuI 563 AGGCCT AGG^CCT
Van91I 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 676 GTCTAC GT^[AC][GT]AC
895 GTCTAC
AflIII 458 ACGTGT A^C[GA][CT]GT
1010 ACACGT
BanII 94 GAGCCC G[GA]GC[CT]^C
1173 GAGCTC
BspHI 737 TCATGA T^CATGA
1160 TCATGA
BspMI 53 ACCTGC ACCTGC(4/8)
413 ACCTGC
BsrBI 355 CCGCTC CCGCTC(-3/-3)
109 GAGCGG
BsrFI 220 GCCGGC [GA]^CCGG[CT]
241 GCCGGC
BsrI 548 ACTGG ACTGG(1/-1)
918 CCAGT
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
241 GCCGGC
DpnII 105 GATC ^GATC
981 GATC
DraII 635 AGGCCCT [GA]G^G[ACGT]CC[CT]
992 GGGACCT
EaeI 467 TGGCCA [CT]^GGCC[GA]
1050 CGGCCG
EcoNI 159 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
401 CCTTGGTGAGG
EcoO109I 635 AGGCCCT [GA]G^G[ACGT]CC[CT]
992 GGGACCT
EcoT14I 401 CCTTGG C^C[AT][AT]GG
464 CCATGG
MboI 105 GATC ^GATC
981 GATC
NaeI 220 GCCGGC GCC^GGC
241 GCCGGC
NdeII 105 GATC ^GATC
981 GATC
NgoMIV 220 GCCGGC G^CCGGC
241 GCCGGC
NspI 329 GCATGT [GA]CATG^[CT]
443 GCATGC
RcaI 737 TCATGA T^CATGA
1160 TCATGA
Sau3AI 105 GATC ^GATC
981 GATC
StyI 401 CCTTGG C^C[AT][AT]GG
464 CCATGG
TaqI 77 TCGA T^CGA
107 TCGA
TspRI 229 CAGTG CA[GC]TG[ACGT][ACGT]^
547 CACTG
TthHB8I 77 TCGA T^CGA
107 TCGA
XcmI 428 CCAAGAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCATGGCCAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BbeI
BbvI BmyI BpmI BsaAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
Bsp1286I BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DsaI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI KspI MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NlaIII NlaIV
PstI RsaI SacII Sau96I ScrFI
SfaNI SfcI SfoI TseI