Restriction map for
pF1KB6596
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SmlI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 856 GTCTAC GT^[AC][GT]AC
AccII 228 CGCG CG^CG
Alw44I 762 GTGCAC G^TGCAC
ApaI 1088 GGGCCC GGGCC^C
ApaLI 762 GTGCAC G^TGCAC
AvaI 685 CCCGGG C^[CT]CG[GA]G
AvaII 465 GGTCC G^G[AT]CC
BbsI 346 GTCTTC GAAGAC(2/6)
BfaI 1138 CTAG C^TAG
BglI 235 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 825 AGATCT A^GATCT
BpuAI 346 GTCTTC GAAGAC(2/6)
BsaBI 159 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 959 TCCGGT [AT]^CCGG[AT]
BsmI 247 GCATTC GAATGC(1/-1)
BsoBI 685 CCCGGG C^[CT]CG[GA]G
BspMI 852 GCAGGT ACCTGC(4/8)
BsrFI 982 GCCGGC [GA]^CCGG[CT]
BstEII 129 GGTGACC G^GT[ACGT]ACC
BstPI 129 GGTGACC G^GT[ACGT]ACC
BstUI 228 CGCG CG^CG
Cfr10I 982 GCCGGC [GA]^CCGG[CT]
DraIII 1163 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 239 CCATGG C^C[GA][CT]GG
EarI 1158 CTCTTC CTCTTC(1/4)
EcoNI 380 CCTTTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 129 GGTGACC G^GT[ACGT]ACC
EcoT22I 641 ATGCAT ATGCA^T
HaeII 979 AGCGCC [GA]GCGC^[CT]
Ksp632I 1158 CTCTTC CTCTTC(1/4)
MaeI 1138 CTAG C^TAG
MamI 159 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 228 CGCG CG^CG
NaeI 982 GCCGGC GCC^GGC
NcoI 239 CCATGG C^CATGG
NgoMIV 982 GCCGGC G^CCGGC
NsiI 641 ATGCAT ATGCA^T
NspI 951 GCATGC [GA]CATG^[CT]
PpuMI 464 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 107 CAGCTG CAG^CTG
SacI 885 GAGCTC GAGCT^C
SapI 1157 GCTCTTC GCTCTTC(1/4)
SfaNI 558 GCATC GCATC(5/9)
SgrAI 981 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 685 CCCGGG CCC^GGG
SphI 951 GCATGC GCATG^C
StuI 233 AGGCCT AGG^CCT
TaqI 545 TCGA T^CGA
TfiI 659 GAATC G^A[AT]TC
Tsp509I 93 AATT ^AATT
TthHB8I 545 TCGA T^CGA
XmaCI 685 CCCGGG C^CCGGG
XmaI 685 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 681 GATCC GGATC(4/5)
164 GGATC
BanII 885 GAGCTC G[GA]GC[CT]^C
1088 GGGCCC
BsaI 514 GAGACC GGTCTC(1/5)
1063 GAGACC
BsmFI 689 GGGAC GGGAC(10/14)
1134 GTCCC
Bsp1407I 896 TGTACA T^GTACA
1043 TGTACA
BsrGI 896 TGTACA T^GTACA
1043 TGTACA
BsrI 353 CCAGT ACTGG(1/-1)
674 CCAGT
BssSI 709 CACGAG CACGAG(-5/-1)
1093 CACGAG
BstF5I 406 CATCC GGATG(2/0)
559 CATCC
BstXI 437 CCACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1076 CCACAGTGGTGG
BstYI 680 AGATCC [GA]^GATC[CT]
825 AGATCT
CfoI 729 GCGC GCG^C
980 GCGC
DdeI 24 CTCAG C^T[ACGT]AG
171 CTTAG
DraII 464 GGGTCCT [GA]G^G[ACGT]CC[CT]
1087 GGGGCCC
EcoO109I 464 GGGTCCT [GA]G^G[ACGT]CC[CT]
1087 GGGGCCC
EcoT14I 239 CCATGG C^C[AT][AT]GG
1118 CCAAGG
FokI 406 CATCC GGATG(9/13)
559 CATCC
HhaI 729 GCGC GCG^C
980 GCGC
HinP1I 729 GCGC G^CGC
980 GCGC
MaeII 8 ACGT A^CGT
149 ACGT
MflI 680 AGATCC [GA]^GATC[CT]
825 AGATCT
MspA1I 107 CAGCTG C[AC]G^C[GT]G
341 CCGCTG
MwoI 235 GCCTCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
343 GCTGTCTTCGC
PflMI 773 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCATCATGTGG
SspBI 896 TGTACA T^GTACA
1043 TGTACA
StyI 239 CCATGG C^C[AT][AT]GG
1118 CCAAGG
Van91I 773 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCATCATGTGG
XhoII 680 AGATCC [GA]^GATC[CT]
825 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AspHI BalI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DpnII EaeI EcoRII Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspI MvaI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI XcmI