Restriction map for
pF1KE2365
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AvrII BbeI BbrPI BbvCI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI MaeI MaeII MamI
MfeI MluI MroI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 535 GGCGTC G[GA]^CG[CT]C
AlwNI 697 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 492 GGGCCC GGGCC^C
AvaI 364 CCCGAG C^[CT]CG[GA]G
AviII 149 TGCGCA TGC^GCA
BalI 566 TGGCCA TGG^CCA
BamHI 716 GGATCC G^GATCC
BanI 177 GGCACC G^G[CT][GA]CC
BbsI 325 GAAGAC GAAGAC(2/6)
BcgI 347 GCATGATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 670 GGATAC GTATCC(6/5)
BcnI 599 CCCGG CC^[GC]GG
BpmI 64 CTCCAG CTGGAG(16/14)
BpuAI 325 GAAGAC GAAGAC(2/6)
BsaHI 535 GGCGTC G[GA]^CG[CT]C
BsaI 481 GAGACC GGTCTC(1/5)
BsiEI 4 CGGCCG CG[GA][CT]^CG
BsoBI 364 CCCGAG C^[CT]CG[GA]G
BspHI 611 TCATGA T^CATGA
BsrI 158 ACTGG ACTGG(1/-1)
BssSI 199 CACGAG CACGAG(-5/-1)
BstEII 467 GGTTACC G^GT[ACGT]ACC
BstPI 467 GGTTACC G^GT[ACGT]ACC
BstXI 98 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 716 GGATCC [GA]^GATC[CT]
DraII 491 AGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 4 CGGCCG C^GGCCG
EclXI 4 CGGCCG C^GGCCG
Eco52I 4 CGGCCG C^GGCCG
EcoO109I 491 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 467 GGTTACC G^GT[ACGT]ACC
FspI 149 TGCGCA TGC^GCA
HaeII 170 AGCGCC [GA]GCGC^[CT]
HgaI 536 GCGTC GACGC(5/10)
Hin1I 535 GGCGTC G[GA]^CG[CT]C
HinfI 220 GATTC G^A[ACGT]TC
KspI 7 CCGCGG CCGC^GG
MflI 716 GGATCC [GA]^GATC[CT]
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MseI 707 TTAA T^TAA
NaeI 584 GCCGGC GCC^GGC
NciI 599 CCCGG CC^[GC]GG
NgoMIV 584 GCCGGC G^CCGGC
NotI 3 GCGGCCGC GC^GGCCGC
NspI 146 ACATGC [GA]CATG^[CT]
RcaI 611 TCATGA T^CATGA
SacII 7 CCGCGG CCGC^GG
SfcI 675 CTACAG C^T[GA][CT]AG
TfiI 220 GATTC G^A[AT]TC
Tru9I 707 TTAA T^TAA
XcmI 657 CCAATGCCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 716 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 8 CGCG CG^CG
30 CGCG
AlwI 717 GATCC GGATC(4/5)
716 GGATC
BseRI 19 CTCCTC GAGGAG(10/8)
591 GAGGAG
BsmAI 165 GAGAC GTCTC(1/5)
481 GAGAC
BsmFI 232 GGGAC GGGAC(10/14)
652 GTCCC
BstF5I 570 CATCC GGATG(2/0)
282 GGATG
BstUI 8 CGCG CG^CG
30 CGCG
DpnII 351 GATC ^GATC
717 GATC
EarI 247 GAAGAG CTCTTC(1/4)
453 GAAGAG
FokI 570 CATCC GGATG(9/13)
282 GGATG
Ksp632I 247 GAAGAG CTCTTC(1/4)
453 GAAGAG
MboI 351 GATC ^GATC
717 GATC
MslI 84 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
309 CACAAGCGTG
MvnI 8 CGCG CG^CG
30 CGCG
NcoI 48 CCATGG C^CATGG
712 CCATGG
NdeII 351 GATC ^GATC
717 GATC
PvuII 34 CAGCTG CAG^CTG
391 CAGCTG
Sau3AI 351 GATC ^GATC
717 GATC
SmlI 448 CTCAAG C^T[CT][GA]AG
623 CTCAAG
Tsp45I 188 GTGAC ^GT[GC]AC
194 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrFI
BssKI BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DsaI EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI TseI TspRI