Restriction map for
pF1KE2532
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# NO CUT ENZYMES ( 65 enzymes) #
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Acc65I AclI AgeI Alw44I ApaI
ApaLI AscI Asp718I AviII AvrII
BbeI BglI BglII BlnI BspHI
BsrBI BssHII Bst1107I BstZ17I CpoI
DraI DraIII DrdI EcoRI EcoT22I
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfoI
SgrAI SmaI SnaBI SpeI SspI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 2314 GTAGAC GT^[AC][GT]AC
AccIII 422 TCCGGA T^CCGGA
AflII 2307 CTTAAG C^TTAAG
AhdI 61 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 946 ATTAAT AT^TAAT
AsnI 946 ATTAAT AT^TAAT
AspEI 61 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 721 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1990 CCCGAG C^[CT]CG[GA]G
BfrI 2307 CTTAAG C^TTAAG
BlpI 3363 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3363 GCTGAGC GC^T[ACGT]AGC
BsaWI 422 TCCGGA [AT]^CCGG[AT]
BseAI 422 TCCGGA T^CCGGA
BsiWI 3320 CGTACG C^GTACG
BsmBI 4265 GAGACG CGTCTC(1/5)
BsoBI 1990 CCCGAG C^[CT]CG[GA]G
BspDI 4429 ATCGAT AT^CGAT
BspEI 422 TCCGGA T^CCGGA
BspLU11I 179 ACATGT A^CATGT
CelII 3363 GCTGAGC GC^T[ACGT]AGC
ClaI 4429 ATCGAT AT^CGAT
EagI 3599 CGGCCG C^GGCCG
Eam1105I 61 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3599 CGGCCG C^GGCCG
Eco52I 3599 CGGCCG C^GGCCG
HincII 2458 GTCAAC GT[CT]^[GA]AC
HindII 2458 GTCAAC GT[CT]^[GA]AC
HindIII 3561 AAGCTT A^AGCTT
MroI 422 TCCGGA T^CCGGA
MslI 3726 CACGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 721 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 946 ATTAAT AT^TAAT
PvuII 2872 CAGCTG CAG^CTG
SacI 682 GAGCTC GAGCT^C
ScaI 5711 AGTACT AGT^ACT
SexAI 4384 ACCAGGT A^CC[AT]GGT
Tth111I 721 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4259 CCATTGGAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AatII 1315 GACGTC GACGT^C
3604 GACGTC
Aor51HI 1862 AGCGCT AGC^GCT
5720 AGCGCT
BbrPI 1786 CACGTG CAC^GTG
2134 CACGTG
BclI 533 TGATCA T^GATCA
3008 TGATCA
BsaBI 2878 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4779 GATTTCCATC
BsiEI 725 CGGTCG CG[GA][CT]^CG
3599 CGGCCG
BsmI 757 GAATGC GAATGC(1/-1)
3434 GAATGC
Bsp1407I 1349 TGTACA T^GTACA
4742 TGTACA
BsrFI 338 ACCGGC [GA]^CCGG[CT]
1657 ACCGGC
BsrGI 1349 TGTACA T^GTACA
4742 TGTACA
BssSI 1042 CACGAG CACGAG(-5/-1)
5136 CTCGTG
BstAPI 1872 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4153 GCAGCTTCTGC
BstBI 2413 TTCGAA TT^CGAA
2677 TTCGAA
Cfr10I 338 ACCGGC [GA]^CCGG[CT]
1657 ACCGGC
Eco47III 1862 AGCGCT AGC^GCT
5720 AGCGCT
EcoRV 673 GATATC GAT^ATC
3085 GATATC
FbaI 533 TGATCA T^GATCA
3008 TGATCA
HaeII 1862 AGCGCT [GA]GCGC^[CT]
5720 AGCGCT
MamI 2878 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4779 GATTTCCATC
NspV 2413 TTCGAA TT^CGAA
2677 TTCGAA
PmaCI 1786 CACGTG CAC^GTG
2134 CACGTG
PmlI 1786 CACGTG CAC^GTG
2134 CACGTG
SapI 888 GCTCTTC GCTCTTC(1/4)
4563 GCTCTTC
SbfI 1131 CCTGCAGG CCTGCA^GG
1509 CCTGCAGG
SfuI 2413 TTCGAA TT^CGAA
2677 TTCGAA
SphI 2267 GCATGC GCATG^C
4088 GCATGC
Sse8387I 1131 CCTGCAGG CCTGCA^GG
1509 CCTGCAGG
SspBI 1349 TGTACA T^GTACA
4742 TGTACA
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# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI