Restriction map for
pF1KE1246
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbvCI BcgI BciVI
BfrI BglI BlnI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1418 GTCGAC GT^[AC][GT]AC
AcsI 365 AAATTC [GA]^AATT[CT]
AcyI 392 GGCGCC G[GA]^CG[CT]C
AhdI 1300 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 924 GATCC GGATC(4/5)
AlwNI 757 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 365 AAATTC [GA]^AATT[CT]
AspEI 1300 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 457 GTGCTC G[AT]GC[AT]^C
BbeI 392 GGCGCC GGCGC^C
BbrPI 483 CACGTG CAC^GTG
BfaI 1402 CTAG C^TAG
BlpI 38 GCTCAGC GC^T[ACGT]AGC
BpmI 494 CTCCAG CTGGAG(16/14)
Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC
BsaAI 483 CACGTG [CT]AC^GT[GA]
BsaHI 392 GGCGCC G[GA]^CG[CT]C
BsiHKAI 457 GTGCTC G[AT]GC[AT]^C
BsmFI 675 GTCCC GGGAC(10/14)
BstAPI 756 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 150 GGTCACC G^GT[ACGT]ACC
BstPI 150 GGTCACC G^GT[ACGT]ACC
CelII 38 GCTCAGC GC^T[ACGT]AGC
DraII 1200 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 691 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1300 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 805 CTCTTC CTCTTC(1/4)
EcoO109I 1200 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 150 GGTCACC G^GT[ACGT]ACC
EcoRV 1174 GATATC GAT^ATC
FauI 653 GCGGG CCCGC(4/6)
HgaI 222 GCGTC GACGC(5/10)
Hin1I 392 GGCGCC G[GA]^CG[CT]C
KasI 392 GGCGCC G^GCGCC
Ksp632I 805 CTCTTC CTCTTC(1/4)
MaeI 1402 CTAG C^TAG
NarI 392 GGCGCC GG^CGCC
NspI 965 ACATGC [GA]CATG^[CT]
PflMI 138 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 483 CACGTG CAC^GTG
PmlI 483 CACGTG CAC^GTG
SalI 1418 GTCGAC G^TCGAC
SapI 804 GCTCTTC GCTCTTC(1/4)
SfoI 392 GGCGCC GGC^GCC
SmaI 677 CCCGGG CCC^GGG
TfiI 336 GAATC G^A[AT]TC
Van91I 138 CCAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 677 CCCGGG C^CCGGG
XmaI 677 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AvaI 677 CCCGGG C^[CT]CG[GA]G
1243 CCCGAG
AvaII 581 GGACC G^G[AT]CC
1353 GGACC
BalI 474 TGGCCA TGG^CCA
602 TGGCCA
BanI 392 GGCGCC G^G[CT][GA]CC
574 GGCACC
BanII 4 GAGCCC G[GA]GC[CT]^C
57 GGGCTC
BclI 665 TGATCA T^GATCA
1232 TGATCA
BglII 246 AGATCT A^GATCT
386 AGATCT
BsaI 70 GAGACC GGTCTC(1/5)
1285 GAGACC
BseRI 16 CTCCTC GAGGAG(10/8)
1363 CTCCTC
BsmAI 70 GAGAC GTCTC(1/5)
1285 GAGAC
BsoBI 677 CCCGGG C^[CT]CG[GA]G
1243 CCCGAG
BspMI 836 ACCTGC ACCTGC(4/8)
1414 GCAGGT
BsrBI 1035 CCGCTC CCGCTC(-3/-3)
651 GAGCGG
BstYI 246 AGATCT [GA]^GATC[CT]
386 AGATCT
CfoI 393 GCGC GCG^C
759 GCGC
DraI 94 TTTAAA TTT^AAA
505 TTTAAA
DrdI 589 GACGGCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1287 GACCATGAAGTC
EcoT14I 977 CCATGG C^C[AT][AT]GG
1211 CCATGG
FbaI 665 TGATCA T^GATCA
1232 TGATCA
HaeII 392 GGCGCC [GA]GCGC^[CT]
758 AGCGCC
HapII 613 CCGG C^CGG
678 CCGG
HhaI 393 GCGC GCG^C
759 GCGC
HinP1I 393 GCGC G^CGC
759 GCGC
HincII 781 GTCAAC GT[CT]^[GA]AC
1418 GTCGAC
HindII 781 GTCAAC GT[CT]^[GA]AC
1418 GTCGAC
HpaII 613 CCGG C^CGG
678 CCGG
MflI 246 AGATCT [GA]^GATC[CT]
386 AGATCT
MluNI 474 TGGCCA TGG^CCA
602 TGGCCA
MscI 474 TGGCCA TGG^CCA
602 TGGCCA
MspI 613 CCGG C^CGG
678 CCGG
NcoI 977 CCATGG C^CATGG
1211 CCATGG
StyI 977 CCATGG C^C[AT][AT]GG
1211 CCATGG
Tsp509I 366 AATT ^AATT
1430 AATT
XhoII 246 AGATCT [GA]^GATC[CT]
386 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I