Restriction map for
pF1KB8499
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BcgI BclI
BcnI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI FauI FbaI
FseI FspI HaeII HapII Hin1I
HindIII HpaII KasI KpnI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflII 555 CTTAAG C^TTAAG
AspHI 1733 GTGCTC G[AT]GC[AT]^C
AvaII 1012 GGACC G^G[AT]CC
BalI 378 TGGCCA TGG^CCA
BanI 106 GGTGCC G^G[CT][GA]CC
BbsI 1827 GAAGAC GAAGAC(2/6)
BbvCI 310 GCTGAGG CCTCAGC(-5/-2)
BfrI 555 CTTAAG C^TTAAG
BmyI 1733 GTGCTC G[AGT]GC[ACT]^C
BpmI 130 CTGGAG CTGGAG(16/14)
BpuAI 1827 GAAGAC GAAGAC(2/6)
BseRI 523 CTCCTC GAGGAG(10/8)
BsiHKAI 1733 GTGCTC G[AT]GC[AT]^C
BsmAI 2522 GTCTC GTCTC(1/5)
BsmI 1299 GAATGC GAATGC(1/-1)
Bsp1286I 1733 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 570 TGTACA T^GTACA
BspDI 895 ATCGAT AT^CGAT
BspMI 1453 GCAGGT ACCTGC(4/8)
BsrDI 208 GCAATG GCAATG(2/0)
BsrGI 570 TGTACA T^GTACA
BssKI 2470 CCAGG ^CC[ACGT]GG
BstNI 2470 CCAGG CC^[AT]GG
Cac8I 12 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 1973 GCGC GCG^C
ClaI 895 ATCGAT AT^CGAT
EaeI 378 TGGCCA [CT]^GGCC[GA]
EcoRII 2470 CCAGG ^CC[AT]GG
EcoRV 811 GATATC GAT^ATC
HgaI 2646 GCGTC GACGC(5/10)
HhaI 1973 GCGC GCG^C
HinP1I 1973 GCGC G^CGC
HpaI 2331 GTTAAC GTT^AAC
MaeII 2369 ACGT A^CGT
MfeI 7 CAATTG C^AATTG
MluNI 378 TGGCCA TGG^CCA
MscI 378 TGGCCA TGG^CCA
MunI 7 CAATTG C^AATTG
MvaI 2470 CCAGG CC^[AT]GG
MwoI 187 GCTGTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 106 GGTGCC GG[ACGT]^[ACGT]CC
SalI 791 GTCGAC G^TCGAC
ScaI 215 AGTACT AGT^ACT
ScrFI 2470 CCAGG CC^[ACGT]GG
SspBI 570 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 791 GTCGAC GT^[AC][GT]AC
2603 GTAGAC
AciI 1061 CCGC CCGC(-3/-1)
16 GCGG
BbvI 1063 GCAGC GCAGC(8/12)
337 GCTGC
BciVI 539 GTATCC GTATCC(6/5)
873 GTATCC
BglII 246 AGATCT A^GATCT
519 AGATCT
BsaJI 95 CCTTGG C^C[ACGT][ACGT]GG
287 CCTTGG
BsiYI 1970 CCAGCGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2527 CCATTCTATGG
BslI 1970 CCAGCGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2527 CCATTCTATGG
BsmFI 643 GGGAC GGGAC(10/14)
2049 GTCCC
BspHI 240 TCATGA T^CATGA
527 TCATGA
Cfr13I 1012 GGACC G^G[ACGT]CC
1986 GGCCC
EcoT14I 95 CCTTGG C^C[AT][AT]GG
287 CCTTGG
HaeIII 379 GGCC GG^CC
1986 GGCC
HphI 1240 GGTGA GGTGA(8/7)
1224 TCACC
MspA1I 908 CAGCTG C[AC]G^C[GT]G
1064 CAGCTG
PflMI 1970 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2527 CCATTCTATGG
PstI 1481 CTGCAG CTGCA^G
2303 CTGCAG
PvuII 908 CAGCTG CAG^CTG
1064 CAGCTG
RcaI 240 TCATGA T^CATGA
527 TCATGA
Sau96I 1012 GGACC G^G[ACGT]CC
1986 GGCCC
SmlI 555 CTTAAG C^T[CT][GA]AG
2284 CTTGAG
SpeI 748 ACTAGT A^CTAGT
1786 ACTAGT
SspI 1531 AATATT AAT^ATT
2077 AATATT
StyI 95 CCTTGG C^C[AT][AT]GG
287 CCTTGG
TseI 337 GCTGC G^C[AT]GC
1063 GCAGC
Van91I 1970 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2527 CCATTCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BfaI BsrI BstF5I BstYI
DdeI DpnII DraI EarI EcoT22I
Fnu4HI FokI HincII HindII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
NdeII NlaIII NsiI PleI RsaI
Sau3AI SfaNI SfcI TaqI TfiI
Tru9I Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI