Restriction map for
pF1KE3426
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BsaAI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MaeI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 88 GACGTC GACGT^C
Acc65I 305 GGTACC G^GTACC
AccIII 393 TCCGGA T^CCGGA
AcsI 514 AAATTT [GA]^AATT[CT]
ApoI 514 AAATTT [GA]^AATT[CT]
Asp718I 305 GGTACC G^GTACC
AvaI 1642 CTCGAG C^[CT]CG[GA]G
BanII 1062 GAGCTC G[GA]GC[CT]^C
BglI 1731 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 110 AGATCT A^GATCT
BpmI 1633 CTGGAG CTGGAG(16/14)
BsaBI 203 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 393 TCCGGA T^CCGGA
BsiEI 1182 CGGCCG CG[GA][CT]^CG
BsiWI 65 CGTACG C^GTACG
BsoBI 1642 CTCGAG C^[CT]CG[GA]G
BspEI 393 TCCGGA T^CCGGA
BspHI 92 TCATGA T^CATGA
BsrBI 1735 GAGCGG CCGCTC(-3/-3)
BsrDI 449 GCAATG GCAATG(2/0)
BssSI 533 CTCGTG CACGAG(-5/-1)
BstXI 179 CCAGCATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 110 AGATCT [GA]^GATC[CT]
DrdI 207 GACATCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1182 CGGCCG C^GGCCG
EclXI 1182 CGGCCG C^GGCCG
Eco52I 1182 CGGCCG C^GGCCG
KpnI 305 GGTACC GGTAC^C
MamI 203 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 110 AGATCT [GA]^GATC[CT]
MroI 393 TCCGGA T^CCGGA
NspI 668 ACATGC [GA]CATG^[CT]
PaeR7I 1642 CTCGAG C^TCGAG
PshAI 1515 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 92 TCATGA T^CATGA
SacI 1062 GAGCTC GAGCT^C
SapI 1335 GCTCTTC GCTCTTC(1/4)
SexAI 366 ACCAGGT A^CC[AT]GGT
SfiI 1730 GGCCTGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 1643 TCGA T^CGA
Tsp509I 515 AATT ^AATT
TthHB8I 1643 TCGA T^CGA
XhoI 1642 CTCGAG C^TCGAG
XhoII 110 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 14 GGATC GGATC(4/5)
1082 GGATC
BbvCI 1551 CCTCAGC CCTCAGC(-5/-2)
1594 CCTCAGC
BlpI 54 GCTCAGC GC^T[ACGT]AGC
757 GCTGAGC
Bpu1102I 54 GCTCAGC GC^T[ACGT]AGC
757 GCTGAGC
BsaWI 393 TCCGGA [AT]^CCGG[AT]
1208 ACCGGA
BssHII 696 GCGCGC G^CGCGC
783 GCGCGC
CelII 54 GCTCAGC GC^T[ACGT]AGC
757 GCTGAGC
DraII 161 AGGTCCT [GA]G^G[ACGT]CC[CT]
1621 GGGACCC
DraIII 365 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1044 CACCGGGTG
EcoO109I 161 AGGTCCT [GA]G^G[ACGT]CC[CT]
1621 GGGACCC
MaeII 89 ACGT A^CGT
487 ACGT
NaeI 826 GCCGGC GCC^GGC
1180 GCCGGC
NcoI 1493 CCATGG C^CATGG
1661 CCATGG
NgoMIV 826 GCCGGC G^CCGGC
1180 GCCGGC
PpuMI 161 AGGTCCT [GA]G^G[AT]CC[CT]
1621 GGGACCC
PstI 166 CTGCAG CTGCA^G
840 CTGCAG
PvuII 456 CAGCTG CAG^CTG
1597 CAGCTG
StuI 1707 AGGCCT AGG^CCT
1721 AGGCCT
TfiI 190 GAATC G^A[AT]TC
894 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BalI BanI BbeI
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TseI Tsp45I
TspRI