Restriction map for
pF1KE2755
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HphI ItaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI MvnI MwoI NaeI NarI
NciI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
TseI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 387 AAATTC [GA]^AATT[CT]
Alw44I 23 GTGCAC G^TGCAC
AlwI 360 GGATC GGATC(4/5)
AlwNI 161 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 23 GTGCAC G^TGCAC
ApoI 387 AAATTC [GA]^AATT[CT]
BanII 60 GAGCTC G[GA]GC[CT]^C
BciVI 350 GTATCC GTATCC(6/5)
BfaI 211 CTAG C^TAG
BsaI 263 GGTCTC GGTCTC(1/5)
BsaJI 356 CCATGG C^C[ACGT][ACGT]GG
BsgI 127 GTGCAG GTGCAG(16/14)
BsmFI 207 GTCCC GGGAC(10/14)
BsrFI 377 ACCGGC [GA]^CCGG[CT]
BsrI 8 ACTGG ACTGG(1/-1)
BssKI 36 CCTGG ^CC[ACGT]GG
BstNI 36 CCTGG CC^[AT]GG
Cac8I 286 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 377 ACCGGC [GA]^CCGG[CT]
DsaI 356 CCATGG C^C[GA][CT]GG
EarI 151 GAAGAG CTCTTC(1/4)
EcoRII 36 CCTGG ^CC[AT]GG
EcoT14I 356 CCATGG C^C[AT][AT]GG
HaeIII 102 GGCC GG^CC
HapII 378 CCGG C^CGG
HincII 132 GTCAAC GT[CT]^[GA]AC
HindII 132 GTCAAC GT[CT]^[GA]AC
HpaII 378 CCGG C^CGG
Ksp632I 151 GAAGAG CTCTTC(1/4)
MaeI 211 CTAG C^TAG
MslI 120 CATAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 284 CAGCTG C[AC]G^C[GT]G
MspI 378 CCGG C^CGG
MvaI 36 CCTGG CC^[AT]GG
NcoI 356 CCATGG C^CATGG
NlaIII 357 CATG CATG^
PvuII 284 CAGCTG CAG^CTG
SacI 60 GAGCTC GAGCT^C
ScrFI 36 CCTGG CC^[ACGT]GG
SfcI 293 CTGTAG C^T[GA][CT]AG
StuI 101 AGGCCT AGG^CCT
StyI 356 CCATGG C^C[AT][AT]GG
TfiI 109 GATTC G^A[AT]TC
Tsp45I 43 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 61 AGCT AG^CT
285 AGCT
AspHI 23 GTGCAC G[AT]GC[AT]^C
60 GAGCTC
BmyI 23 GTGCAC G[AGT]GC[ACT]^C
60 GAGCTC
BpmI 9 CTGGAG CTGGAG(16/14)
113 CTGGAG
BsiHKAI 23 GTGCAC G[AT]GC[AT]^C
60 GAGCTC
BsiYI 140 CCACAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCATGGATCGG
BslI 140 CCACAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
356 CCATGGATCGG
BsmAI 229 GAGAC GTCTC(1/5)
264 GTCTC
Bsp1286I 23 GTGCAC G[AGT]GC[ACT]^C
60 GAGCTC
BstF5I 33 CATCC GGATG(2/0)
331 CATCC
FokI 33 CATCC GGATG(9/13)
331 CATCC
MaeIII 43 GTGAC ^GT[ACGT]AC
88 GTAAC
MboII 151 GAAGA GAAGA(8/7)
237 GAAGA
PleI 278 GACTC GAGTC(4/5)
116 GAGTC
Tsp509I 200 AATT ^AATT
388 AATT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
DdeI DpnII HinfI MboI MnlI
NdeII Sau3AI TspRI