Restriction map for
pF1KE2457
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AlwI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BciVI
BclI BglII BlnI BseAI BsiWI
BsmI BspEI BspHI BspLU11I BssHII
Bst1107I BstEII BstPI BstYI BstZ17I
CpoI DraI DraIII Eam1105I EcoO65I
EcoRV FbaI FseI HindIII HpaI
KpnI MfeI MflI MroI MunI
NdeI NheI NotI NruI PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 3223 GTCTAC GT^[AC][GT]AC
AcsI 897 GAATTC [GA]^AATT[CT]
AcyI 262 GGCGCC G[GA]^CG[CT]C
AflII 2808 CTTAAG C^TTAAG
AflIII 3392 ACGCGT A^C[GA][CT]GT
Alw44I 2724 GTGCAC G^TGCAC
AocI 1873 CCTGAGG CC^T[ACGT]AGG
Aor51HI 785 AGCGCT AGC^GCT
ApaLI 2724 GTGCAC G^TGCAC
ApoI 897 GAATTC [GA]^AATT[CT]
AviII 2069 TGCGCA TGC^GCA
BalI 381 TGGCCA TGG^CCA
BbeI 262 GGCGCC GGCGC^C
BfrI 2808 CTTAAG C^TTAAG
BlpI 1475 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1475 GCTCAGC GC^T[ACGT]AGC
BsaBI 2982 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 262 GGCGCC G[GA]^CG[CT]C
BsaWI 1657 ACCGGA [AT]^CCGG[AT]
BsiEI 2493 CGGCCG CG[GA][CT]^CG
Bsp1407I 2420 TGTACA T^GTACA
BspDI 2938 ATCGAT AT^CGAT
BsrGI 2420 TGTACA T^GTACA
BssSI 2094 CACGAG CACGAG(-5/-1)
BstAPI 2338 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 835 TTCGAA TT^CGAA
Bsu36I 1873 CCTGAGG CC^T[ACGT]AGG
CelII 1475 GCTCAGC GC^T[ACGT]AGC
ClaI 2938 ATCGAT AT^CGAT
EagI 2493 CGGCCG C^GGCCG
EclXI 2493 CGGCCG C^GGCCG
Eco47III 785 AGCGCT AGC^GCT
Eco52I 2493 CGGCCG C^GGCCG
Eco81I 1873 CCTGAGG CC^T[ACGT]AGG
EcoRI 897 GAATTC G^AATTC
EcoT22I 2046 ATGCAT ATGCA^T
FspI 2069 TGCGCA TGC^GCA
Hin1I 262 GGCGCC G[GA]^CG[CT]C
KasI 262 GGCGCC G^GCGCC
KspI 3053 CCGCGG CCGC^GG
MamI 2982 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3392 ACGCGT A^CGCGT
MluNI 381 TGGCCA TGG^CCA
MscI 381 TGGCCA TGG^CCA
MseI 2809 TTAA T^TAA
NaeI 321 GCCGGC GCC^GGC
NarI 262 GGCGCC GG^CGCC
NgoMIV 321 GCCGGC G^CCGGC
NsiI 2046 ATGCAT ATGCA^T
NspV 835 TTCGAA TT^CGAA
PaeR7I 967 CTCGAG C^TCGAG
PshAI 2382 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3053 CCGCGG CCGC^GG
SapI 987 GAAGAGC GCTCTTC(1/4)
SfiI 624 GGCCAGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 262 GGCGCC GGC^GCC
SfuI 835 TTCGAA TT^CGAA
SspBI 2420 TGTACA T^GTACA
Tru9I 2809 TTAA T^TAA
XhoI 967 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 2772 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2936 GAATCGATTC
AspI 3112 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3217 GACCTGGTC
BanI 262 GGCGCC G^G[CT][GA]CC
2485 GGCACC
BbvCI 2224 GCTGAGG CCTCAGC(-5/-2)
3514 GCTGAGG
BcgI 2950 GCACCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3380 GCATGAAACTCG
BsaI 1026 GGTCTC GGTCTC(1/5)
1689 GGTCTC
DrdI 87 GACAACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2298 GACACCCAAGTC
EcoNI 226 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1446 CCTGCTTCAGG
HaeII 262 GGCGCC [GA]GCGC^[CT]
785 AGCGCT
HincII 2791 GTCAAC GT[CT]^[GA]AC
2863 GTCAAC
HindII 2791 GTCAAC GT[CT]^[GA]AC
2863 GTCAAC
NcoI 2852 CCATGG C^CATGG
3282 CCATGG
NspI 69 GCATGT [GA]CATG^[CT]
2900 ACATGC
PflFI 3112 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3217 GACCTGGTC
SacI 2269 GAGCTC GAGCT^C
2628 GAGCTC
SexAI 1022 ACCAGGT A^CC[AT]GGT
3218 ACCTGGT
Tsp509I 898 AATT ^AATT
2665 AATT
Tth111I 3112 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3217 GACCTGGTC
XmnI 2772 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2936 GAATCGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbrPI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI