Restriction map for
pF1KB6281
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AccII AccIII AclI
AlwNI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FbaI FseI HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MslI MspA1I MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 611 GACGTC GACGT^C
AcyI 611 GACGTC G[GA]^CG[CT]C
AflIII 11 ACATGT A^C[GA][CT]GT
AgeI 1441 ACCGGT A^CCGGT
AhdI 25 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2115 GTGCAC G^TGCAC
ApaLI 2115 GTGCAC G^TGCAC
AspEI 25 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1207 GAGCTC G[GA]GC[CT]^C
BglII 93 AGATCT A^GATCT
BpmI 1769 CTCCAG CTGGAG(16/14)
BsaHI 611 GACGTC G[GA]^CG[CT]C
BsaWI 1441 ACCGGT [AT]^CCGG[AT]
BsgI 171 GTGCAG GTGCAG(16/14)
BsmI 478 GAATGC GAATGC(1/-1)
BspLU11I 11 ACATGT A^CATGT
BsrFI 1441 ACCGGT [GA]^CCGG[CT]
BstEII 1144 GGTGACC G^GT[ACGT]ACC
BstPI 1144 GGTGACC G^GT[ACGT]ACC
Cfr10I 1441 ACCGGT [GA]^CCGG[CT]
DraII 1708 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 786 GACCCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1517 CCATGG C^C[GA][CT]GG
Eam1105I 25 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1708 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1144 GGTGACC G^GT[ACGT]ACC
EcoRI 558 GAATTC G^AATTC
FauI 2130 GCGGG CCCGC(4/6)
HaeII 2024 AGCGCC [GA]GCGC^[CT]
HgaI 2255 GACGC GACGC(5/10)
Hin1I 611 GACGTC G[GA]^CG[CT]C
HincII 590 GTCAAC GT[CT]^[GA]AC
HindII 590 GTCAAC GT[CT]^[GA]AC
HindIII 2382 AAGCTT A^AGCTT
NcoI 1517 CCATGG C^CATGG
NheI 264 GCTAGC G^CTAGC
NspI 11 ACATGT [GA]CATG^[CT]
PinAI 1441 ACCGGT A^CCGGT
PpuMI 1708 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1721 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 872 CTGCAG CTGCA^G
SacI 1207 GAGCTC GAGCT^C
StuI 876 AGGCCT AGG^CCT
XbaI 1219 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflII 430 CTTAAG C^TTAAG
849 CTTAAG
AseI 1168 ATTAAT AT^TAAT
1192 ATTAAT
AsnI 1168 ATTAAT AT^TAAT
1192 ATTAAT
AspHI 1207 GAGCTC G[AT]GC[AT]^C
2115 GTGCAC
AvaII 1709 GGTCC G^G[AT]CC
2082 GGACC
AviII 2213 TGCGCA TGC^GCA
2387 TGCGCA
BcgI 51 CGAGTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1056 CGAGTTGTATGC
BciVI 138 GGATAC GTATCC(6/5)
2203 GGATAC
BfrI 430 CTTAAG C^TTAAG
849 CTTAAG
BmyI 1207 GAGCTC G[AGT]GC[ACT]^C
2115 GTGCAC
BsiHKAI 1207 GAGCTC G[AT]GC[AT]^C
2115 GTGCAC
BsmBI 422 CGTCTC CGTCTC(1/5)
344 GAGACG
Bsp1286I 1207 GAGCTC G[AGT]GC[ACT]^C
2115 GTGCAC
BspMI 1326 GCAGGT ACCTGC(4/8)
2216 GCAGGT
BssSI 1102 CACGAG CACGAG(-5/-1)
1924 CACGAG
FspI 2213 TGCGCA TGC^GCA
2387 TGCGCA
HapII 1045 CCGG C^CGG
1442 CCGG
HpaII 1045 CCGG C^CGG
1442 CCGG
MfeI 542 CAATTG C^AATTG
2015 CAATTG
MspI 1045 CCGG C^CGG
1442 CCGG
MunI 542 CAATTG C^AATTG
2015 CAATTG
PshBI 1168 ATTAAT AT^TAAT
1192 ATTAAT
SapI 415 GAAGAGC GCTCTTC(1/4)
2020 GAAGAGC
TaqI 50 TCGA T^CGA
1719 TCGA
TthHB8I 50 TCGA T^CGA
1719 TCGA
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BanI BbsI BbvI BfaI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII