Restriction map for
pF1KE2369
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AccIII AclI AgeI
AlwI AocI Aor51HI ApaI AseI
AsnI Asp700I AspI AviII AvrII
BalI BamHI BglI BglII BlnI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI Bst1107I BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DsaI
Eco47III Eco81I EcoO109I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1363 AAATTC [GA]^AATT[CT]
AflII 792 CTTAAG C^TTAAG
AhdI 484 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1219 GTGCAC G^TGCAC
ApaLI 1219 GTGCAC G^TGCAC
ApoI 1363 AAATTC [GA]^AATT[CT]
AscI 374 GGCGCGCC GG^CGCGCC
AspEI 484 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1579 GGGCTC G[GA]GC[CT]^C
BbeI 475 GGCGCC GGCGC^C
BbrPI 1216 CACGTG CAC^GTG
BbvCI 185 CCTCAGC CCTCAGC(-5/-2)
BcgI 536 CGATCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1236 GGATAC GTATCC(6/5)
BfrI 792 CTTAAG C^TTAAG
BsaAI 1216 CACGTG [CT]AC^GT[GA]
BsaI 457 GAGACC GGTCTC(1/5)
BsiEI 34 CGGCCG CG[GA][CT]^CG
BspHI 1229 TCATGA T^CATGA
BspMI 11 GCAGGT ACCTGC(4/8)
BssHII 375 GCGCGC G^CGCGC
BssSI 73 CACGAG CACGAG(-5/-1)
BstBI 385 TTCGAA TT^CGAA
BstXI 230 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 34 CGGCCG [CT]^GGCC[GA]
EagI 34 CGGCCG C^GGCCG
Eam1105I 484 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 34 CGGCCG C^GGCCG
Eco52I 34 CGGCCG C^GGCCG
EcoNI 1682 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1056 GATATC GAT^ATC
EcoT14I 1038 CCTTGG C^C[AT][AT]GG
HphI 1087 GGTGA GGTGA(8/7)
KasI 475 GGCGCC G^GCGCC
NarI 475 GGCGCC GG^CGCC
NotI 33 GCGGCCGC GC^GGCCGC
NspI 428 GCATGT [GA]CATG^[CT]
NspV 385 TTCGAA TT^CGAA
PflMI 72 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1216 CACGTG CAC^GTG
PmlI 1216 CACGTG CAC^GTG
RcaI 1229 TCATGA T^CATGA
SfoI 475 GGCGCC GGC^GCC
SfuI 385 TTCGAA TT^CGAA
SgrAI 370 CACCGGCG C[GA]^CCGG[CT]G
StyI 1038 CCTTGG C^C[AT][AT]GG
Van91I 72 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 856 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 1004 GGTACC G^GTACC
1095 GGTACC
AflIII 895 ACACGT A^C[GA][CT]GT
1215 ACACGT
Asp718I 1004 GGTACC G^GTACC
1095 GGTACC
AvaI 193 CCCGAG C^[CT]CG[GA]G
214 CCCGAG
BbsI 909 GAAGAC GAAGAC(2/6)
1504 GAAGAC
BclI 554 TGATCA T^GATCA
1011 TGATCA
BcnI 719 CCCGG CC^[GC]GG
1676 CCCGG
BpmI 272 CTGGAG CTGGAG(16/14)
615 CTGGAG
BpuAI 909 GAAGAC GAAGAC(2/6)
1504 GAAGAC
BsmFI 57 GTCCC GGGAC(10/14)
1674 GTCCC
BsoBI 193 CCCGAG C^[CT]CG[GA]G
214 CCCGAG
BsrFI 253 ACCGGC [GA]^CCGG[CT]
371 ACCGGC
BstAPI 93 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
151 GCAGCCGCTGC
Cfr10I 253 ACCGGC [GA]^CCGG[CT]
371 ACCGGC
DrdI 459 GACCGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1645 GACAAGCACGTC
EarI 1704 CTCTTC CTCTTC(1/4)
1515 GAAGAG
FbaI 554 TGATCA T^GATCA
1011 TGATCA
HaeII 240 GGCGCT [GA]GCGC^[CT]
475 GGCGCC
KpnI 1004 GGTACC GGTAC^C
1095 GGTACC
Ksp632I 1704 CTCTTC CTCTTC(1/4)
1515 GAAGAG
MslI 704 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1434 CACTTTGGTG
NciI 719 CCCGG CC^[GC]GG
1676 CCCGG
SmlI 48 CTTGAG C^T[CT][GA]AG
792 CTTAAG
Tsp45I 1088 GTGAC ^GT[GC]AC
1654 GTCAC
XcmI 752 CCACCGCTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1566 CCACGCCCTCCCTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BbvI
BfaI BlpI BmyI Bpu1102I BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I