Restriction map for
pF1KB8472
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspHI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BciVI BcnI BlnI BmyI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmFI Bsp1286I
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI NarI
NciI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 601 GTAGAC GT^[AC][GT]AC
AccII 55 CGCG CG^CG
AflII 397 CTTAAG C^TTAAG
AflIII 1587 ACATGT A^C[GA][CT]GT
AhdI 763 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1345 CCTCAGG CC^T[ACGT]AGG
AseI 507 ATTAAT AT^TAAT
AsnI 507 ATTAAT AT^TAAT
Asp700I 388 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 763 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1118 CTCGAG C^[CT]CG[GA]G
BalI 668 TGGCCA TGG^CCA
BcgI 1064 GCAAATTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 397 CTTAAG C^TTAAG
BglI 1699 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 846 AGATCT A^GATCT
BlpI 2452 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2452 GCTGAGC GC^T[ACGT]AGC
BsaI 1648 GAGACC GGTCTC(1/5)
BsiEI 93 CGACCG CG[GA][CT]^CG
BsiYI 2413 CCAGTGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 2413 CCAGTGGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 1118 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1787 TGTACA T^GTACA
BspLU11I 1587 ACATGT A^CATGT
BspMI 1733 ACCTGC ACCTGC(4/8)
BsrBI 1837 GAGCGG CCGCTC(-3/-3)
BsrGI 1787 TGTACA T^GTACA
BstUI 55 CGCG CG^CG
BstXI 1721 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1345 CCTCAGG CC^T[ACGT]AGG
CelII 2452 GCTGAGC GC^T[ACGT]AGC
CfoI 56 GCGC GCG^C
DraI 199 TTTAAA TTT^AAA
DraII 2439 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1246 GACTGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 763 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1345 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2439 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1218 GAATTC G^AATTC
FauI 53 CCCGC CCCGC(4/6)
HhaI 56 GCGC GCG^C
HinP1I 56 GCGC G^CGC
MfeI 140 CAATTG C^AATTG
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MunI 140 CAATTG C^AATTG
MvnI 55 CGCG CG^CG
NspI 1587 ACATGT [GA]CATG^[CT]
PaeR7I 1118 CTCGAG C^TCGAG
PflMI 2413 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 507 ATTAAT AT^TAAT
SgrAI 57 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1787 TGTACA T^GTACA
StuI 1328 AGGCCT AGG^CCT
Van91I 2413 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2357 CCAAACCCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1118 CTCGAG C^TCGAG
XmnI 388 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 1364 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1631 CAGAGTCTG
BamHI 1663 GGATCC G^GATCC
2277 GGATCC
BbsI 1716 GAAGAC GAAGAC(2/6)
1990 GAAGAC
BclI 1811 TGATCA T^GATCA
2169 TGATCA
BpuAI 1716 GAAGAC GAAGAC(2/6)
1990 GAAGAC
BsgI 99 CTGCAC GTGCAG(16/14)
274 GTGCAG
BsmI 1542 GAATGC GAATGC(1/-1)
220 GCATTC
BsrFI 58 GCCGGC [GA]^CCGG[CT]
105 GCCGGC
Cfr10I 58 GCCGGC [GA]^CCGG[CT]
105 GCCGGC
EaeI 213 TGGCCG [CT]^GGCC[GA]
668 TGGCCA
FbaI 1811 TGATCA T^GATCA
2169 TGATCA
HapII 59 CCGG C^CGG
106 CCGG
HincII 412 GTTGAC GT[CT]^[GA]AC
2045 GTCAAC
HindII 412 GTTGAC GT[CT]^[GA]AC
2045 GTCAAC
HindIII 1891 AAGCTT A^AGCTT
2497 AAGCTT
HpaII 59 CCGG C^CGG
106 CCGG
HphI 306 GGTGA GGTGA(8/7)
1900 GGTGA
MaeII 41 ACGT A^CGT
2061 ACGT
MspI 59 CCGG C^CGG
106 CCGG
NaeI 58 GCCGGC GCC^GGC
105 GCCGGC
NdeI 263 CATATG CA^TATG
539 CATATG
NgoMIV 58 GCCGGC G^CCGGC
105 GCCGGC
PleI 794 GACTC GAGTC(4/5)
1633 GAGTC
PstI 524 CTGCAG CTGCA^G
2251 CTGCAG
PvuII 1226 CAGCTG CAG^CTG
2471 CAGCTG
SapI 322 GAAGAGC GCTCTTC(1/4)
1594 GAAGAGC
ScaI 1723 AGTACT AGT^ACT
2329 AGTACT
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BpmI
BsaJI BseRI BsmAI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII