Restriction map for
pF1KE2359
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# NO CUT ENZYMES ( 146 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI Asp718I AspEI AspHI AspI
AvaII AvrII BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HgaI HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspBI StyI
SwaI Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 298 GACGTC GACGT^C
AcyI 298 GACGTC G[GA]^CG[CT]C
AflIII 1065 ACATGT A^C[GA][CT]GT
Aor51HI 995 AGCGCT AGC^GCT
Asp700I 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1111 CTCGAG C^[CT]CG[GA]G
AviII 407 TGCGCA TGC^GCA
BalI 95 TGGCCA TGG^CCA
BcnI 914 CCGGG CC^[GC]GG
BmyI 815 GGGCAC G[AGT]GC[ACT]^C
BsaAI 543 TACGTA [CT]AC^GT[GA]
BsaBI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GACGTC G[GA]^CG[CT]C
BsaI 760 GAGACC GGTCTC(1/5)
BsgI 1087 CTGCAC GTGCAG(16/14)
BsmAI 760 GAGAC GTCTC(1/5)
BsoBI 1111 CTCGAG C^[CT]CG[GA]G
Bsp1286I 815 GGGCAC G[AGT]GC[ACT]^C
BspDI 318 ATCGAT AT^CGAT
BspLU11I 1065 ACATGT A^CATGT
Cfr13I 1020 GGGCC G^G[ACGT]CC
ClaI 318 ATCGAT AT^CGAT
DrdI 856 GACTACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 95 TGGCCA [CT]^GGCC[GA]
Eco47III 995 AGCGCT AGC^GCT
FspI 407 TGCGCA TGC^GCA
HaeII 995 AGCGCT [GA]GCGC^[CT]
HapII 914 CCGG C^CGG
Hin1I 298 GACGTC G[GA]^CG[CT]C
HincII 562 GTCAAC GT[CT]^[GA]AC
HindII 562 GTCAAC GT[CT]^[GA]AC
HpaII 914 CCGG C^CGG
MamI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 95 TGGCCA TGG^CCA
MscI 95 TGGCCA TGG^CCA
MslI 786 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 914 CCGG C^CGG
NciI 914 CCGGG CC^[GC]GG
PaeR7I 1111 CTCGAG C^TCGAG
PflMI 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 1020 GGGCC G^G[ACGT]CC
SfcI 384 CTATAG C^T[GA][CT]AG
SmlI 1111 CTCGAG C^T[CT][GA]AG
SnaBI 543 TACGTA TAC^GTA
SphI 663 GCATGC GCATG^C
SspI 835 AATATT AAT^ATT
StuI 715 AGGCCT AGG^CCT
Van91I 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1111 CTCGAG C^TCGAG
XmnI 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 639 CCGC CCGC(-3/-1)
193 GCGG
AseI 417 ATTAAT AT^TAAT
1051 ATTAAT
AsnI 417 ATTAAT AT^TAAT
1051 ATTAAT
BamHI 843 GGATCC G^GATCC
1057 GGATCC
BanI 229 GGTGCC G^G[CT][GA]CC
816 GGCACC
BbsI 1004 GAAGAC GAAGAC(2/6)
553 GTCTTC
BpuAI 1004 GAAGAC GAAGAC(2/6)
553 GTCTTC
BstNI 868 CCTGG CC^[AT]GG
1146 CCTGG
BstXI 233 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAATATTGTGG
CfoI 408 GCGC GCG^C
996 GCGC
EcoRII 868 CCTGG ^CC[AT]GG
1146 CCTGG
HhaI 408 GCGC GCG^C
996 GCGC
HinP1I 408 GCGC G^CGC
996 GCGC
HinfI 1141 GAATC G^A[ACGT]TC
1150 GAATC
MboII 1004 GAAGA GAAGA(8/7)
554 TCTTC
MseI 418 TTAA T^TAA
1052 TTAA
MvaI 868 CCTGG CC^[AT]GG
1146 CCTGG
MwoI 213 GCTGGTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
740 GCACTCATCGC
NspI 663 GCATGC [GA]CATG^[CT]
1065 ACATGT
PshBI 417 ATTAAT AT^TAAT
1051 ATTAAT
TfiI 1141 GAATC G^A[AT]TC
1150 GAATC
Tru9I 418 TTAA T^TAA
1052 TTAA
Tsp45I 649 GTCAC ^GT[GC]AC
829 GTCAC
TspRI 47 CAGTG CA[GC]TG[ACGT][ACGT]^
861 CACTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmFI BspMI
BssKI BstF5I BstYI Cac8I DdeI
DpnII Fnu4HI FokI HaeIII HphI
ItaI MaeII MaeIII MboI MflI
MnlI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI TaqI TseI
Tsp509I TthHB8I XhoII