Restriction map for
pF1KE2416
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbrPI
BbvCI BfrI BlnI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1463 GGTACC G^GTACC
Alw44I 1086 GTGCAC G^TGCAC
Aor51HI 1405 AGCGCT AGC^GCT
ApaLI 1086 GTGCAC G^TGCAC
Asp718I 1463 GGTACC G^GTACC
BbsI 1840 GTCTTC GAAGAC(2/6)
BciVI 1451 GTATCC GTATCC(6/5)
BclI 1301 TGATCA T^GATCA
BfaI 1105 CTAG C^TAG
BglII 908 AGATCT A^GATCT
BpuAI 1840 GTCTTC GAAGAC(2/6)
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsaI 519 GGTCTC GGTCTC(1/5)
BsaWI 1035 ACCGGA [AT]^CCGG[AT]
BsrDI 864 CATTGC GCAATG(2/0)
BstEII 465 GGTGACC G^GT[ACGT]ACC
BstPI 465 GGTGACC G^GT[ACGT]ACC
EarI 337 CTCTTC CTCTTC(1/4)
Eco47III 1405 AGCGCT AGC^GCT
EcoO65I 465 GGTGACC G^GT[ACGT]ACC
FbaI 1301 TGATCA T^GATCA
KpnI 1463 GGTACC GGTAC^C
Ksp632I 337 CTCTTC CTCTTC(1/4)
MaeI 1105 CTAG C^TAG
MaeII 92 ACGT A^CGT
MseI 677 TTAA T^TAA
PflMI 49 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1681 CTGCAG CTGCA^G
SfcI 1681 CTGCAG C^T[GA][CT]AG
SfiI 1647 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1804 AGGCCT AGG^CCT
TaqI 744 TCGA T^CGA
Tru9I 677 TTAA T^TAA
TthHB8I 744 TCGA T^CGA
Van91I 49 CCAGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 651 CGCG CG^CG
1783 CGCG
AcyI 292 GGCGCC G[GA]^CG[CT]C
1225 GGCGCC
AfaI 1464 GTAC GT^AC
1530 GTAC
AocI 103 CCTCAGG CC^T[ACGT]AGG
885 CCTGAGG
ApaI 156 GGGCCC GGGCC^C
1646 GGGCCC
BbeI 292 GGCGCC GGCGC^C
1225 GGCGCC
BcgI 1232 GCATGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1634 GCACGCCCTTCG
BlpI 172 GCTGAGC GC^T[ACGT]AGC
360 GCTCAGC
Bpu1102I 172 GCTGAGC GC^T[ACGT]AGC
360 GCTCAGC
BsaHI 292 GGCGCC G[GA]^CG[CT]C
1225 GGCGCC
BsrBI 1324 CCGCTC CCGCTC(-3/-3)
1815 CCGCTC
BstAPI 63 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1885 GCACTGCCTGC
BstUI 651 CGCG CG^CG
1783 CGCG
Bsu36I 103 CCTCAGG CC^T[ACGT]AGG
885 CCTGAGG
CelII 172 GCTGAGC GC^T[ACGT]AGC
360 GCTCAGC
Eco81I 103 CCTCAGG CC^T[ACGT]AGG
885 CCTGAGG
Hin1I 292 GGCGCC G[GA]^CG[CT]C
1225 GGCGCC
KasI 292 GGCGCC G^GCGCC
1225 GGCGCC
MvnI 651 CGCG CG^CG
1783 CGCG
NarI 292 GGCGCC GG^CGCC
1225 GGCGCC
NspI 63 GCATGT [GA]CATG^[CT]
356 ACATGC
PvuII 376 CAGCTG CAG^CTG
838 CAGCTG
RsaI 1464 GTAC GT^AC
1530 GTAC
SacI 483 GAGCTC GAGCT^C
543 GAGCTC
SfoI 292 GGCGCC GGC^GCC
1225 GGCGCC
SmaI 123 CCCGGG CCC^GGG
1696 CCCGGG
XmaCI 123 CCCGGG C^CCGGG
1696 CCCGGG
XmaI 123 CCCGGG C^CCGGG
1696 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TseI Tsp45I TspRI XcmI
XhoII