Restriction map for
pF1KE2049
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AccII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbsI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsiWI BspDI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraIII DrdI Eco47III
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 598 GACGTC GACGT^C
AccIII 813 TCCGGA T^CCGGA
AflIII 11 ACATGT A^C[GA][CT]GT
AhdI 1235 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1345 GTGCAC G^TGCAC
AlwNI 1511 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1355 CCTCAGG CC^T[ACGT]AGG
ApaI 148 GGGCCC GGGCC^C
ApaLI 1345 GTGCAC G^TGCAC
AspEI 1235 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 583 CCCGAG C^[CT]CG[GA]G
BamHI 1437 GGATCC G^GATCC
BbeI 579 GGCGCC GGCGC^C
BbrPI 304 CACGTG CAC^GTG
BbvCI 525 GCTGAGG CCTCAGC(-5/-2)
BclI 125 TGATCA T^GATCA
BcnI 219 CCGGG CC^[GC]GG
BglI 1353 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 304 CACGTG [CT]AC^GT[GA]
BsaWI 813 TCCGGA [AT]^CCGG[AT]
BseAI 813 TCCGGA T^CCGGA
BsiEI 823 CGGCCG CG[GA][CT]^CG
BsmBI 1233 GAGACG CGTCTC(1/5)
BsoBI 583 CCCGAG C^[CT]CG[GA]G
BspEI 813 TCCGGA T^CCGGA
BspLU11I 11 ACATGT A^CATGT
BspMI 1450 GCAGGT ACCTGC(4/8)
BsrFI 992 GCCGGT [GA]^CCGG[CT]
BstYI 1437 GGATCC [GA]^GATC[CT]
Bsu36I 1355 CCTCAGG CC^T[ACGT]AGG
Cfr10I 992 GCCGGT [GA]^CCGG[CT]
DraI 909 TTTAAA TTT^AAA
DsaI 1931 CCACGG C^C[GA][CT]GG
EaeI 823 CGGCCG [CT]^GGCC[GA]
EagI 823 CGGCCG C^GGCCG
Eam1105I 1235 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 823 CGGCCG C^GGCCG
Eco52I 823 CGGCCG C^GGCCG
Eco81I 1355 CCTCAGG CC^T[ACGT]AGG
EcoNI 177 CCTCTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 125 TGATCA T^GATCA
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
KasI 579 GGCGCC G^GCGCC
MaeIII 44 GTGAC ^GT[ACGT]AC
MflI 1437 GGATCC [GA]^GATC[CT]
MroI 813 TCCGGA T^CCGGA
NarI 579 GGCGCC GG^CGCC
NciI 219 CCGGG CC^[GC]GG
NspI 11 ACATGT [GA]CATG^[CT]
PflMI 1457 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 304 CACGTG CAC^GTG
PmlI 304 CACGTG CAC^GTG
PstI 1120 CTGCAG CTGCA^G
PvuII 1703 CAGCTG CAG^CTG
SfoI 579 GGCGCC GGC^GCC
SmlI 1376 CTCAAG C^T[CT][GA]AG
SpeI 1738 ACTAGT A^CTAGT
StuI 856 AGGCCT AGG^CCT
TaqI 1976 TCGA T^CGA
Tsp45I 44 GTGAC ^GT[GC]AC
TthHB8I 1976 TCGA T^CGA
Van91I 1457 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1437 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BpmI 52 CTGGAG CTGGAG(16/14)
1087 CTGGAG
BsaI 1519 GAGACC GGTCTC(1/5)
1242 GGTCTC
BsmI 950 GAATGC GAATGC(1/-1)
1613 GCATTC
Bsp1407I 140 TGTACA T^GTACA
632 TGTACA
BsrGI 140 TGTACA T^GTACA
632 TGTACA
BstXI 1038 CCAGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCATCTTTATGG
CfoI 580 GCGC GCG^C
1954 GCGC
HaeII 579 GGCGCC [GA]GCGC^[CT]
1953 GGCGCT
HgaI 1235 GACGC GACGC(5/10)
239 GCGTC
HhaI 580 GCGC GCG^C
1954 GCGC
HinP1I 580 GCGC G^CGC
1954 GCGC
MspA1I 72 CAGCGG C[AC]G^C[GT]G
1703 CAGCTG
NdeI 680 CATATG CA^TATG
1486 CATATG
PleI 119 GACTC GAGTC(4/5)
1066 GAGTC
SapI 511 GAAGAGC GCTCTTC(1/4)
1696 GAAGAGC
SfaNI 300 GATGC GCATC(5/9)
1890 GATGC
SfcI 644 CTGTAG C^T[GA][CT]AG
1120 CTGCAG
SspBI 140 TGTACA T^GTACA
632 TGTACA
TfiI 810 GAATC G^A[AT]TC
1579 GAATC
XcmI 41 CCAGTGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1196 CCAGACAGAATGTGG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI Tru9I TseI Tsp509I
TspRI