Restriction map for
pF1KE1617
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# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRII EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeIII HgaI Hin1I HincII HindII
HindIII HpaI HphI ItaI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MscI
MslI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 153 TCCGGA T^CCGGA
AcsI 192 GAATTC [GA]^AATT[CT]
AfaI 366 GTAC GT^AC
Aor51HI 260 AGCGCT AGC^GCT
ApoI 192 GAATTC [GA]^AATT[CT]
Asp700I 192 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 346 GGCACC G^G[CT][GA]CC
BcnI 313 CCGGG CC^[GC]GG
BmyI 345 GGGCAC G[AGT]GC[ACT]^C
BsaJI 350 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 153 TCCGGA [AT]^CCGG[AT]
BseAI 153 TCCGGA T^CCGGA
Bsp1286I 345 GGGCAC G[AGT]GC[ACT]^C
BspEI 153 TCCGGA T^CCGGA
BspMI 144 GCAGGT ACCTGC(4/8)
BsrI 160 ACTGG ACTGG(1/-1)
BssKI 313 CCGGG ^CC[ACGT]GG
BstBI 171 TTCGAA TT^CGAA
BstF5I 151 CATCC GGATG(2/0)
DraII 97 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 168 CTCTTC CTCTTC(1/4)
Eco47III 260 AGCGCT AGC^GCT
EcoO109I 97 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 192 GAATTC G^AATTC
EcoRV 208 GATATC GAT^ATC
EcoT14I 350 CCAAGG C^C[AT][AT]GG
FokI 151 CATCC GGATG(9/13)
HaeII 260 AGCGCT [GA]GCGC^[CT]
HinfI 285 GATTC G^A[ACGT]TC
Ksp632I 168 CTCTTC CTCTTC(1/4)
MaeII 240 ACGT A^CGT
MroI 153 TCCGGA T^CCGGA
NciI 313 CCGGG CC^[GC]GG
NlaIII 331 CATG CATG^
NspI 330 ACATGC [GA]CATG^[CT]
NspV 171 TTCGAA TT^CGAA
PpuMI 97 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 366 GTAC GT^AC
ScrFI 313 CCGGG CC^[ACGT]GG
SfcI 252 CTACAG C^T[GA][CT]AG
SfuI 171 TTCGAA TT^CGAA
StyI 350 CCAAGG C^C[AT][AT]GG
TaqI 172 TCGA T^CGA
TfiI 285 GATTC G^A[AT]TC
Tsp509I 193 AATT ^AATT
TthHB8I 172 TCGA T^CGA
XcmI 150 CCATCCGGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 192 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 98 GGACC G^G[AT]CC
147 GGTCC
BsiYI 69 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
154 CCGGACACTGG
BslI 69 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
154 CCGGACACTGG
Cfr13I 98 GGACC G^G[ACGT]CC
147 GGTCC
DdeI 341 CTGAG C^T[ACGT]AG
362 CTAAG
MaeIII 202 GTCAC ^GT[ACGT]AC
358 GTCAC
MboII 169 TCTTC GAAGA(8/7)
274 TCTTC
MseI 47 TTAA T^TAA
62 TTAA
NlaIV 98 GGACCC GG[ACGT]^[ACGT]CC
346 GGCACC
Sau96I 98 GGACC G^G[ACGT]CC
147 GGTCC
SfaNI 164 GCATC GCATC(5/9)
235 GCATC
Tru9I 47 TTAA T^TAA
62 TTAA
Tsp45I 202 GTCAC ^GT[GC]AC
358 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI Cac8I CfoI HapII HhaI
HinP1I HpaII MnlI MspI MwoI
TspRI