Restriction map for
pF1KE2311
##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI Aor51HI ApaI AscI
AspEI AviII AvrII BamHI BbeI
BbrPI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI FseI
FspI KasI KspI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
Sse8387I SspBI SspI SwaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 109 CGCG CG^CG
AcyI 4788 GGCGTC G[GA]^CG[CT]C
AflII 660 CTTAAG C^TTAAG
Alw44I 770 GTGCAC G^TGCAC
AocI 1874 CCTCAGG CC^T[ACGT]AGG
ApaLI 770 GTGCAC G^TGCAC
AseI 2581 ATTAAT AT^TAAT
AsnI 2581 ATTAAT AT^TAAT
AspI 77 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2176 CCCGAG C^[CT]CG[GA]G
BalI 4875 TGGCCA TGG^CCA
BbvCI 3542 CCTCAGC CCTCAGC(-5/-2)
BcgI 2466 CGAAGTAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 216 TGATCA T^GATCA
BfrI 660 CTTAAG C^TTAAG
BsaHI 4788 GGCGTC G[GA]^CG[CT]C
BsiEI 34 CGATCG CG[GA][CT]^CG
BsmBI 1968 GAGACG CGTCTC(1/5)
BsmI 4429 GCATTC GAATGC(1/-1)
BsoBI 2176 CCCGAG C^[CT]CG[GA]G
BsrBI 2137 GAGCGG CCGCTC(-3/-3)
BsrDI 435 CATTGC GCAATG(2/0)
BsrFI 4029 GCCGGT [GA]^CCGG[CT]
BstUI 109 CGCG CG^CG
Bsu36I 1874 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4029 GCCGGT [GA]^CCGG[CT]
DraI 1180 TTTAAA TTT^AAA
DrdI 2247 GACTTACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1874 CCTCAGG CC^T[ACGT]AGG
FbaI 216 TGATCA T^GATCA
HaeII 947 AGCGCC [GA]GCGC^[CT]
Hin1I 4788 GGCGTC G[GA]^CG[CT]C
HindIII 4658 AAGCTT A^AGCTT
HpaI 412 GTTAAC GTT^AAC
MluNI 4875 TGGCCA TGG^CCA
MscI 4875 TGGCCA TGG^CCA
MvnI 109 CGCG CG^CG
PflFI 77 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 180 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 542 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2581 ATTAAT AT^TAAT
PvuI 34 CGATCG CGAT^CG
SacI 40 GAGCTC GAGCT^C
ScaI 5055 AGTACT AGT^ACT
SnaBI 3926 TACGTA TAC^GTA
SpeI 1126 ACTAGT A^CTAGT
SphI 1588 GCATGC GCATG^C
StuI 18 AGGCCT AGG^CCT
Tth111I 77 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 180 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3075 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3400 GGTACC G^GTACC
3469 GGTACC
AlwNI 97 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
181 CAGAATCTG
Asp700I 3265 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5053 GAAGTACTTC
Asp718I 3400 GGTACC G^GTACC
3469 GGTACC
AspHI 40 GAGCTC G[AT]GC[AT]^C
770 GTGCAC
BanI 3400 GGTACC G^G[CT][GA]CC
3469 GGTACC
BanII 40 GAGCTC G[GA]GC[CT]^C
3935 GAGCCC
BciVI 441 GGATAC GTATCC(6/5)
1109 GTATCC
BsaAI 1166 CACGTA [CT]AC^GT[GA]
3926 TACGTA
BsaBI 851 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5176 GATGCAGATC
BsgI 834 GTGCAG GTGCAG(16/14)
3429 GTGCAG
BsiHKAI 40 GAGCTC G[AT]GC[AT]^C
770 GTGCAC
BspMI 930 ACCTGC ACCTGC(4/8)
1984 GCAGGT
BstEII 1783 GGTAACC G^GT[ACGT]ACC
3205 GGTGACC
BstPI 1783 GGTAACC G^GT[ACGT]ACC
3205 GGTGACC
BstXI 1533 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2824 CCATCAACATGG
EcoO65I 1783 GGTAACC G^GT[ACGT]ACC
3205 GGTGACC
EcoRV 851 GATATC GAT^ATC
1802 GATATC
EcoT22I 523 ATGCAT ATGCA^T
4604 ATGCAT
FauI 2139 GCGGG CCCGC(4/6)
3657 GCGGG
HgaI 3178 GACGC GACGC(5/10)
4789 GCGTC
KpnI 3400 GGTACC GGTAC^C
3469 GGTACC
MamI 851 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5176 GATGCAGATC
NcoI 2974 CCATGG C^CATGG
4878 CCATGG
NsiI 523 ATGCAT ATGCA^T
4604 ATGCAT
SapI 1197 GAAGAGC GCTCTTC(1/4)
4282 GCTCTTC
XmnI 3265 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5053 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstBI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI NspV PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfuI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII