Restriction map for
pF1KB5966
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BfrI BglII BlnI BsaBI
BsaWI BseAI BsgI BsiWI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1453 GTCTAC GT^[AC][GT]AC
AccII 99 CGCG CG^CG
AlwI 1325 GGATC GGATC(4/5)
BanI 222 GGTGCC G^G[CT][GA]CC
BbsI 121 GTCTTC GAAGAC(2/6)
BcgI 1070 CGAGCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1178 TGATCA T^GATCA
BlpI 1363 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1363 GCTGAGC GC^T[ACGT]AGC
BpuAI 121 GTCTTC GAAGAC(2/6)
BsaI 1570 GAGACC GGTCTC(1/5)
BseRI 1022 CTCCTC GAGGAG(10/8)
BsiEI 1194 CGACCG CG[GA][CT]^CG
BsmBI 385 GAGACG CGTCTC(1/5)
BsmFI 368 GGGAC GGGAC(10/14)
BsmI 885 GAATGC GAATGC(1/-1)
Bsp1407I 1055 TGTACA T^GTACA
BspHI 1175 TCATGA T^CATGA
BsrFI 1457 ACCGGC [GA]^CCGG[CT]
BsrGI 1055 TGTACA T^GTACA
BssSI 1068 CACGAG CACGAG(-5/-1)
BstUI 99 CGCG CG^CG
CelII 1363 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1457 ACCGGC [GA]^CCGG[CT]
DsaI 11 CCGTGG C^C[GA][CT]GG
EarI 970 GAAGAG CTCTTC(1/4)
EcoRI 1159 GAATTC G^AATTC
EcoRV 695 GATATC GAT^ATC
FbaI 1178 TGATCA T^GATCA
HindIII 1559 AAGCTT A^AGCTT
Ksp632I 970 GAAGAG CTCTTC(1/4)
MvnI 99 CGCG CG^CG
NheI 753 GCTAGC G^CTAGC
PpuMI 234 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1175 TCATGA T^CATGA
SmlI 1477 CTTGAG C^T[CT][GA]AG
SspBI 1055 TGTACA T^GTACA
XcmI 592 CCAACCTATACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 679 GAATTT [GA]^AATT[CT]
1159 GAATTC
AlwNI 233 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
955 CAGCGCCTG
ApaI 228 GGGCCC GGGCC^C
357 GGGCCC
ApoI 679 GAATTT [GA]^AATT[CT]
1159 GAATTC
AspI 16 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1009 GACTTGGTC
BalI 320 TGGCCA TGG^CCA
581 TGGCCA
BfaI 754 CTAG C^TAG
1648 CTAG
BglI 365 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1249 GCCTGTCTGGC
BsaAI 109 TACGTG [CT]AC^GT[GA]
600 TACGTG
BspMI 237 ACCTGC ACCTGC(4/8)
1114 GCAGGT
BstXI 5 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
104 CCATCTACGTGG
EcoT14I 627 CCAAGG C^C[AT][AT]GG
1216 CCTTGG
HaeII 269 GGCGCT [GA]GCGC^[CT]
956 AGCGCC
MaeI 754 CTAG C^TAG
1648 CTAG
MaeII 110 ACGT A^CGT
601 ACGT
MluNI 320 TGGCCA TGG^CCA
581 TGGCCA
MscI 320 TGGCCA TGG^CCA
581 TGGCCA
PflFI 16 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1009 GACTTGGTC
PflMI 431 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAAGGACTGG
PstI 200 CTGCAG CTGCA^G
563 CTGCAG
PvuII 566 CAGCTG CAG^CTG
1166 CAGCTG
SacI 1071 GAGCTC GAGCT^C
1480 GAGCTC
SexAI 419 ACCTGGT A^CC[AT]GGT
1272 ACCTGGT
StyI 627 CCAAGG C^C[AT][AT]GG
1216 CCTTGG
Tth111I 16 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1009 GACTTGGTC
Van91I 431 CCAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAAGGACTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I