Restriction map for
pF1KE1824
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BsrDI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MluI MluNI
MroI MscI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 537 GGTACC G^GTACC
ApaI 832 GGGCCC GGGCC^C
Asp718I 537 GGTACC G^GTACC
AvaI 121 CTCGAG C^[CT]CG[GA]G
BanII 832 GGGCCC G[GA]GC[CT]^C
BbsI 556 GAAGAC GAAGAC(2/6)
BciVI 906 GTATCC GTATCC(6/5)
BclI 761 TGATCA T^GATCA
BmyI 832 GGGCCC G[AGT]GC[ACT]^C
BpmI 827 CTCCAG CTGGAG(16/14)
BpuAI 556 GAAGAC GAAGAC(2/6)
BsaWI 349 ACCGGA [AT]^CCGG[AT]
BsgI 206 GTGCAG GTGCAG(16/14)
BsiYI 835 CCCGAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 835 CCCGAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 228 GGGAC GGGAC(10/14)
BsoBI 121 CTCGAG C^[CT]CG[GA]G
Bsp1286I 832 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 167 TGTACA T^GTACA
BspHI 80 TCATGA T^CATGA
BspMI 540 ACCTGC ACCTGC(4/8)
BsrBI 124 GAGCGG CCGCTC(-3/-3)
BsrGI 167 TGTACA T^GTACA
BsrI 688 ACTGG ACTGG(1/-1)
BstAPI 960 GCACGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 953 TTCGAA TT^CGAA
BstYI 107 GGATCT [GA]^GATC[CT]
CfoI 697 GCGC GCG^C
DdeI 479 CTCAG C^T[ACGT]AG
EarI 504 GAAGAG CTCTTC(1/4)
FauI 104 GCGGG CCCGC(4/6)
FbaI 761 TGATCA T^GATCA
HhaI 697 GCGC GCG^C
HinP1I 697 GCGC G^CGC
KpnI 537 GGTACC GGTAC^C
Ksp632I 504 GAAGAG CTCTTC(1/4)
MfeI 426 CAATTG C^AATTG
MflI 107 GGATCT [GA]^GATC[CT]
MslI 436 CATCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 426 CAATTG C^AATTG
NspV 953 TTCGAA TT^CGAA
PaeR7I 121 CTCGAG C^TCGAG
PpuMI 228 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 777 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 542 CTGCAG CTGCA^G
RcaI 80 TCATGA T^CATGA
SfuI 953 TTCGAA TT^CGAA
SspBI 167 TGTACA T^GTACA
Tsp45I 994 GTGAC ^GT[GC]AC
TspRI 465 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 121 CTCGAG C^TCGAG
XhoII 107 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 164 ACATGT A^C[GA][CT]GT
453 ACATGT
AlwI 107 GGATC GGATC(4/5)
680 GGATC
BfaI 314 CTAG C^TAG
920 CTAG
BseRI 800 CTCCTC GAGGAG(10/8)
403 GAGGAG
BspLU11I 164 ACATGT A^CATGT
453 ACATGT
BsrFI 699 GCCGGT [GA]^CCGG[CT]
1015 ACCGGC
BssKI 666 CCAGG ^CC[ACGT]GG
829 CCAGG
BstF5I 976 CATCC GGATG(2/0)
352 GGATG
BstNI 666 CCAGG CC^[AT]GG
829 CCAGG
Cfr10I 699 GCCGGT [GA]^CCGG[CT]
1015 ACCGGC
DraII 228 GGGACCT [GA]G^G[ACGT]CC[CT]
831 AGGGCCC
EcoO109I 228 GGGACCT [GA]G^G[ACGT]CC[CT]
831 AGGGCCC
EcoRII 666 CCAGG ^CC[AT]GG
829 CCAGG
EcoT14I 306 CCTTGG C^C[AT][AT]GG
676 CCAAGG
FokI 976 CATCC GGATG(9/13)
352 GGATG
HinfI 302 GAATC G^A[ACGT]TC
594 GATTC
HphI 631 TCACC GGTGA(8/7)
804 TCACC
MaeI 314 CTAG C^TAG
920 CTAG
MaeIII 243 GTTAC ^GT[ACGT]AC
994 GTGAC
MvaI 666 CCAGG CC^[AT]GG
829 CCAGG
NspI 164 ACATGT [GA]CATG^[CT]
453 ACATGT
ScrFI 666 CCAGG CC^[ACGT]GG
829 CCAGG
SfcI 542 CTGCAG C^T[GA][CT]AG
609 CTACAG
SmlI 121 CTCGAG C^T[CT][GA]AG
901 CTTGAG
StyI 306 CCTTGG C^C[AT][AT]GG
676 CCAAGG
TaqI 122 TCGA T^CGA
954 TCGA
TfiI 302 GAATC G^A[AT]TC
594 GATTC
TthHB8I 122 TCGA T^CGA
954 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BsaJI Cac8I Cfr13I DpnII
Fnu4HI HaeIII HapII HpaII ItaI
MboI MboII MnlI MseI MspA1I
MspI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I SfaNI
Tru9I TseI Tsp509I